{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T04:48:23Z","timestamp":1777870103689,"version":"3.51.4"},"reference-count":56,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T00:00:00Z","timestamp":1776988800000},"content-version":"vor","delay-in-days":54,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100020907","name":"Instituto Nacional de Salud","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100020907","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Growing evidence suggests an association between SARS\u2010CoV\u20102 infection and oxidative stress\u2013related genomic damage, primarily from cross\u2010sectional studies. However, longitudinal evaluations remain limited. Here, we assessed DNA damage in peripheral blood mononuclear cells (PBMCs) from hospitalized COVID\u201019 patients during the first days of hospitalization, using the alkaline comet assay. A longitudinal descriptive study was conducted at a modular COVID\u201019 hospital in Lima, Peru, throughout October 2020. Blood samples were collected at admission (day 0), day 3, and day 6. DNA damage was assessed by measuring tail length, tail moment, and tail intensity, which were modeled using mixed\u2010effects linear models. Concurrent hematological parameters and acute\u2010phase proteins were also analyzed to characterize their time\u2010course patterns in conjunction with DNA damage. Results from the adjusted mixed\u2010effects model showed a significant temporal increase in DNA damage parameters, particularly tail intensity (\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2009=\u20090.25 per day,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.003), relative to baseline measurements, with a peak observed on day 3 of hospitalization. These changes aligned with variations in platelet counts and transferrin levels, while lymphocyte and monocyte counts, along with C\u2010reactive protein, showed an inverse trend, reflecting the dynamic interplay of the inflammatory response and DNA damage. No significant differences in comet assay parameters were observed between survivors and non\u2010survivors, likely due to the limited and unbalanced sample. The findings indicate the presence of a measurable DNA damage burden in PBMCs of hospitalized COVID\u201019 patients, highlighting the need to further studies to clarify the mechanisms underlying DNA damage and its potential long\u2010term biological implications.\n                  <\/jats:p>","DOI":"10.1002\/em.70055","type":"journal-article","created":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T06:31:52Z","timestamp":1777098712000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Longitudinal Assessment of\n                    <scp>DNA<\/scp>\n                    Damage in\n                    <scp>PBMCs<\/scp>\n                    From Hospitalized\n                    <scp>COVID<\/scp>\n                    \u201019 Patients via Alkaline Comet Assay"],"prefix":"10.1002","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5685-6658","authenticated-orcid":false,"given":"Lenin","family":"Rueda\u2010Torres","sequence":"first","affiliation":[{"name":"Instituto Nacional de Salud, Centro Nacional de Salud Ocupacional y Protecci\u00f3n del Ambiente Para la Salud  Lima Per\u00fa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0300-4683","authenticated-orcid":false,"given":"Dulce E.","family":"Alarc\u00f3n\u2010Yaquetto","sequence":"additional","affiliation":[{"name":"University College London, Institute of Health Informatics  London UK"},{"name":"Universidad Peruana Cayetano Heredia  Lima Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2630-4569","authenticated-orcid":false,"given":"Beatriz","family":"Ayala\u2010Quintanilla","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Salud, Direcci\u00f3n de Investigaci\u00f3n e Innovaci\u00f3n en Salud  Lima Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7828-300X","authenticated-orcid":false,"given":"Germ\u00e1n","family":"M\u00e1laga","sequence":"additional","affiliation":[{"name":"Universidad Peruana Cayetano Heredia  Lima Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1665-2332","authenticated-orcid":false,"given":"Jaime","family":"Rosales\u2010Rimache","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Salud, Centro Nacional de Salud Ocupacional y Protecci\u00f3n del Ambiente Para la Salud  Lima Per\u00fa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,24]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00335\u2010024\u201010082\u2010z"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0296255"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxics11040386"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2025.105728"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.14744\/nci.2022.00947"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.DNAREP.2021.103180"},{"key":"e_1_2_11_8_1","unstructured":"Centro Nacional de Epidemiologia Prevencion y Control de Enfermedades.2020.\u201cAn\u00e1lisis Epidemiol\u00f3gico de la Situaci\u00f3n Actual De COVID\u201019 en el Per\u00fa [WWW Document]. 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