{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T10:17:12Z","timestamp":1787825832318,"version":"build-2784847793"},"reference-count":39,"publisher":"Wiley","issue":"5-7","license":[{"start":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T00:00:00Z","timestamp":1787356800000},"content-version":"vor","delay-in-days":82,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Antibiotics contaminating aquatic environments is a challenge for the ONE Health initiative as it poses a risk to biodiversity. Tetracyclines are toxic to a wide range of organisms. The objective of this study was to assess the growth, individual fitness and DNA damage following a short\u2010term exposure to tetracycline in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Daphnia magna<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Additionally, the long\u2010term effects of DNA damage on population growth rate and individual fitness parameters in the offspring for intergenerations were evaluated. The results indicated that\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>D. magna<\/jats:italic>\n                    <\/jats:styled-content>\n                    has a significant effect on tetracycline\u2010induced fertility impairment, resulting in reduced survival and reproductive capacity of the parental groups and impaired growth and development of the offspring. Furthermore, tetracycline treatment resulted in a significant reduction in individual fitness, as evidenced by the significant reduction in maximum body length of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>D. magna<\/jats:italic>\n                    <\/jats:styled-content>\n                    in both tetracycline\u2010exposed generations.\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>D. magna<\/jats:italic>\n                    <\/jats:styled-content>\n                    was exposed to concentrations of 25, 50, 100, 200, and 400\u2009mg\/L for periods of 24\u201372\u2009h, with the objective of determining the concentrations that would not be lethal and yet would induce DNA damage. Tetracycline concentrations of 50 and 25\u2009mg\/L did not result in excessive levels of cytotoxicity within 72\u2009h. P, F1, and F1 offspring born between 48 and 72\u2009h were tested for DNA damage.\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>D. magna<\/jats:italic>\n                    <\/jats:styled-content>\n                    appears to recover from DNA damage, but concerns have been raised about using tetracycline repeatedly in water, as it can lead to higher concentrations and longer exposure times for lots of organisms.\n                  <\/jats:p>","DOI":"10.1002\/em.70077","type":"journal-article","created":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T09:02:47Z","timestamp":1787389367000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Parental Tetracycline Exposure Induces Genotoxic Damage and Fitness Deficits in\n                    <i>Daphnia magna<\/i>\n                    Offspring"],"prefix":"10.1002","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-8422-0994","authenticated-orcid":false,"given":"Buse Ceyda","family":"\u00d6ncel","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Genetics, Faculty of Science and Letters Ba\u015fkent University  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5736-3062","authenticated-orcid":false,"given":"\u00c7i\u011fdem Ak\u0131n","family":"Pek\u015fen","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics, Faculty of Science and Letters Ba\u015fkent University  Ankara T\u00fcrkiye"},{"name":"Institute of Transplantation and Gene Sciences, Ba\u015fkent University  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1762-6252","authenticated-orcid":false,"given":"Beste","family":"Tunca","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics, Faculty of Science and Letters Ba\u015fkent University  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2488-2699","authenticated-orcid":false,"given":"Zeynep","family":"Kavaso\u011flu","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics, Faculty of Science and Letters Ba\u015fkent University  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3394-2518","authenticated-orcid":false,"given":"\u00d6zlem Darcansoy","family":"\u0130\u015feri","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics, Faculty of Science and Letters Ba\u015fkent University  Ankara T\u00fcrkiye"},{"name":"Institute of Food, Agriculture and Livestock Development, Ba\u015fkent University  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,22]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010025\u201092692\u20104"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1211\/jpp.59.9.0008"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.14744\/eej.2018.47966"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2004.12.013"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.seppur.2011.09.029"},{"key":"e_1_2_11_7_1","unstructured":"BioRender.2024.https:\/\/app.biorender.com\/illustrations\/660e7c0feca68ddf1a3df5e7."},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.1966.01600190083018"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/P29790000369"},{"key":"e_1_2_11_10_1","volume-title":"Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information","author":"Ebert D.","year":"2005"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166\u2010445X(90)90009\u2010E"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/10.11.2019"},{"key":"e_1_2_11_13_1","unstructured":"Ersoy M. 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