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The flavanolignan silymarin, which is extracted from milk thistle, has well\u2010known antioxidant and cytoprotective effects. This study examined the protective activity of silymarin against daunorubicin\u2010induced primary damage to DNA in lymphocytes of rats. Adult Wistar rats were divided into four groups and administered silymarin by oral gavage and daunorubicin by a single intraperitoneal injection. Isolation of peripheral\u2010blood lymphocytes was followed by alkaline single\u2010cell gel electrophoresis (comet assay). The amount of DNA damage was determined as the percentage of DNA in the comet tail and was analyzed at the animal level using parametric and nonparametric statistics. DNA migration was extensive when treated with daunorubicin. The administration of silymarin in combination significantly reduced this damage, and it changed the profile of cellular damage from extreme to mostly moderate. There was no significant increase in DN damage in animals given silymarin. Silymarin has a clear antigenotoxic effect in\u00a0vivo in the presence of daunorubicin, and it could be a useful chemoprotective adjuvant.<\/jats:p>","DOI":"10.1002\/em.70080","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T06:53:09Z","timestamp":1790319189000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Silymarin Protects Against Daunorubicin\u2010Induced\n                    <scp>DNA<\/scp>\n                    Damage in an In\u00a0Vivo Comet Assay"],"prefix":"10.1002","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-5020-0477","authenticated-orcid":false,"given":"Muhammad","family":"Mustafa","sequence":"first","affiliation":[{"name":"Institute of Zoological Sciences University of Peshawar  Peshawar Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6144-1989","authenticated-orcid":false,"given":"Aiman","family":"Tariq","sequence":"additional","affiliation":[{"name":"Department of Biosciences COMSATS University Islamabad  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0495-4173","authenticated-orcid":false,"given":"Muhammad","family":"Khisroon","sequence":"additional","affiliation":[{"name":"Institute of Zoological Sciences University of Peshawar  Peshawar Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8798-4941","authenticated-orcid":false,"given":"Aiman","family":"Mehroon","sequence":"additional","affiliation":[{"name":"Institute of Zoological Sciences University of Peshawar  Peshawar Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8418-8228","authenticated-orcid":false,"given":"Aaliya","family":"Saleem","sequence":"additional","affiliation":[{"name":"MEDIcen Project Pakistan Council of Scientific &amp; Industrial Research Laboratories Complex  Peshawar Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2561-0089","authenticated-orcid":false,"given":"Bushra","family":"Arshad","sequence":"additional","affiliation":[{"name":"APIs Project Pakistan Council of Scientific &amp; Industrial Research Laboratories  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,24]]},"reference":[{"issue":"1","key":"e_1_2_10_2_1","doi-asserted-by":"crossref","DOI":"10.1186\/s12885-019-5377-y","article-title":"Time\u2010Dependent Response of Daunorubicin on Cytotoxicity, Cell Cycle, and DNA Repair in Acute Lymphoblastic Leukaemia","volume":"19","author":"Al\u2010Aamri H. 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