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This study was designed to define the impact of\n                    <jats:italic>Trp53<\/jats:italic>\n                    status upon the manifestation of mutations in the\n                    <jats:italic>hypoxanthine\u2010guanine phosphoribosyltransferase<\/jats:italic>\n                    (\n                    <jats:italic>Hprt<\/jats:italic>\n                    ) gene in splenic T\u2010cells following exposure of\n                    <jats:italic>Trp53<\/jats:italic>\n                    wild\u2010type, hemizygous, and nullizygous\n                    <jats:italic>lacZ<\/jats:italic>\n                    transgenic mice to 1\u2009Gy\n                    <jats:sup>56<\/jats:sup>\n                    Fe particles. In male and female mice necropsied at 2, 4, and 8\u2009weeks postexposure,\n                    <jats:italic>Hprt<\/jats:italic>\n                    mutant frequencies in splenic T\u2010cells (measured via the\n                    <jats:italic>Hprt<\/jats:italic>\n                    mutation cloning assay) increased significantly over time to peak at 4\u2009weeks postexposure before decreasing to lower but still elevated levels. Mutagenic potency estimates based upon the change in mutant frequency over time in exposed versus respective control groups showed that the total mutagenic response depended upon the\n                    <jats:italic>Trp53<\/jats:italic>\n                    genetic status, with the mutagenic burden of 1\u2009Gy being threefold and 5.3\u2010fold greater in\n                    <jats:italic>Trp53<\/jats:italic>\n                    <jats:sup>+\/\u2212<\/jats:sup>\n                    and\n                    <jats:italic>Trp53<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice, respectively, compared to\n                    <jats:italic>Trp53<\/jats:italic>\n                    <jats:sup>+\/+<\/jats:sup>\n                    mice.\n                  <\/jats:p>","DOI":"10.1002\/em.70084","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T07:01:27Z","timestamp":1791356487000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>Trp53<\/i>\n                    Deficiency Increases the Frequency of\n                    <i>Hprt<\/i>\n                    Mutant T Lymphocytes Following High\u2010\n                    <scp>LET<\/scp>\n                    Iron Particle Irradiation of Mice"],"prefix":"10.1002","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0299-7177","authenticated-orcid":false,"given":"Vernon E.","family":"Walker","sequence":"first","affiliation":[{"name":"New York State Department of Health Wadsworth Center  Albany New York USA"},{"name":"Department of Pathology and Laboratory Medicine, Larner College of Medicine University of Vermont  Burlington Vermont USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dale M.","family":"Walker","sequence":"additional","affiliation":[{"name":"Experimental Pathology Laboratories  Sterling Virginia USA"},{"name":"The Burlington HC Research Group Inc.  Jericho Vermont USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasily N.","family":"Dobrovolsky","sequence":"additional","affiliation":[{"name":"Division of Genetic and Molecular Toxicology U.S. Food and Drug Administration, National Center for Toxicological Research  Jefferson Arkansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise","family":"Lutze\u2010Mann","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Biomolecular Sciences University of New South Wales  Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,7]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/bcr427"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/01926230600867743"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.asr.2007.05.034"},{"issue":"1","key":"e_1_2_10_5_1","first-page":"189","article-title":"HZE Particle Radiation Induces Tissue\u2010Specific and p53\u2010Dependent Mutagenesis in Transgenic Animals","volume":"17","author":"Chang P. 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