{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T07:38:41Z","timestamp":1792827521565,"version":"4.5.0"},"reference-count":22,"publisher":"Wiley","issue":"8-9","license":[{"start":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:00:00Z","timestamp":1792800000000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["400563\/2021\u20105"],"award-info":[{"award-number":["400563\/2021\u20105"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["304093\/2024\u20106"],"award-info":[{"award-number":["304093\/2024\u20106"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study evaluated the susceptibility to lung and kidney carcinogenesis induced by\n                    <jats:italic>N<\/jats:italic>\n                    \u2010bis(2\u2010hydroxypropyl)nitrosamine (DHPN) in female rats whose dams received a low\u2010protein diet (LPD) during gestation. Pregnant Wistar rats were fed normal protein diet (NPD, 17% protein) or LPD (6% protein). After parturition, all dams were fed NPD until weaning. Then, female offspring were distributed into four groups (NPD, LPD, NPD\u2009+\u2009DHPN, and LPD\u2009+\u2009DHPN) and fed NPD. At 6\u2009weeks old, two groups received 0.2% DHPN in drinking water for 4\u2009weeks, and some females were euthanized 24\u2009h after carcinogen stopping, while others were euthanized at Week 26. First, the lung was removed, fixed, histologically processed, and tissue sections were immunohistochemically stained for \u03b3\u2010H2AX. Second, the lung and kidneys were removed, fixed, histologically processed, and hematoxylin\u2013eosin stained tissue sections were obtained for histopathological analysis. Female rats whose dams received LPD developed a higher mean number of pulmonary epithelial cells positive for \u03b3\u2010H2AX (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.005) and bronchioloalveolar hyperplasia and adenomas (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001 and\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001) in the lung compared with their counterparts. LPD did not alter the development of atypical tubule hyperplasia in the kidney. These findings indicated that LPD increased DHPN\u2010induced lung carcinogenesis in female rats.\n                  <\/jats:p>","DOI":"10.1002\/em.70086","type":"journal-article","created":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T07:23:01Z","timestamp":1792826581000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Gestational Low Protein Diet Increases the Susceptibility to\n                    <i>N<\/i>\n                    \u2010Bis(2\u2010Hydroxypropyl)nitrosamine\u2010Induced Lung Tumors in Female Wistar Rats"],"prefix":"10.1002","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9921-7821","authenticated-orcid":false,"given":"Vanessa de C\u00e1ssia Martins Pires","family":"Ferreira","sequence":"first","affiliation":[{"name":"Department of Cellular and Molecular Biology Institute of Biosciences, UNESP\u2014S\u00e3o Paulo State University  Botucatu SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor Hugo","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology Institute of Biosciences, UNESP\u2014S\u00e3o Paulo State University  Botucatu SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1196-2606","authenticated-orcid":false,"given":"Patr\u00edcia Aline","family":"Boer","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Nucleus of Medicine and Experimental Surgery, FCM UNICAMP\u2014State University of Campinas  Campinas SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2180-1814","authenticated-orcid":false,"given":"Lu\u00eds Fernando","family":"Barbisan","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology Institute of Biosciences, UNESP\u2014S\u00e3o Paulo State University  Botucatu SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0210454"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102796.2007.01809.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2523"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10522\u2010025\u201010316\u2010w"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.892322"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0192623312438736"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2021.119764"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/1535370218758275"},{"key":"e_1_2_9_10_1","first-page":"573","article-title":"Effect of Dose on the Carcinogenic Activity of Orally 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