{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T12:38:41Z","timestamp":1792067921773,"version":"4.4.0"},"reference-count":37,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2013,3,25]],"date-time":"2013-03-25T00:00:00Z","timestamp":1364169600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"},{"start":{"date-parts":[[2013,3,25]],"date-time":"2013-03-25T00:00:00Z","timestamp":1364169600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Wellcome Trust Sanger Institute"},{"name":"NIHR Cambridge Biomedical Research Center"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["EMBO Mol Med"],"published-print":{"date-parts":[[2013,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Several methicillin\u2010resistant\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (MRSA) lineages that carry a novel\n                    <jats:italic>mecA<\/jats:italic>\n                    homologue (\n                    <jats:italic>mecC<\/jats:italic>\n                    ) have recently been described in livestock and humans. In Denmark, two independent human cases of\n                    <jats:italic>mecC<\/jats:italic>\n                    \u2010MRSA infection have been linked to a livestock reservoir. We investigated the molecular epidemiology of the associated MRSA isolates using whole genome sequencing (WGS). Single nucleotide polymorphisms (SNP) were defined and compared to a reference genome to place the isolates into a phylogenetic context. Phylogenetic analysis revealed two distinct farm\u2010specific clusters comprising isolates from the human case and their own livestock, whereas human and animal isolates from the same farm only differed by a small number of SNPs, which supports the likelihood of zoonotic transmission. Further analyses identified a number of genes and mutations that may be associated with host interaction and virulence. This study demonstrates that\n                    <jats:italic>mecC<\/jats:italic>\n                    \u2010MRSA ST130 isolates are capable of transmission between animals and humans, and underscores the potential of WGS in epidemiological investigations and source tracking of bacterial infections.\n                  <\/jats:p>\n                  <jats:p>\n                    \u2192See accompanying article\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/dx.doi.org\/10.1002\/emmm.201302622\" ext-link-type=\"doi\">10.1002\/emmm.201302622<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1002\/emmm.201202413","type":"journal-article","created":{"date-parts":[[2013,3,25]],"date-time":"2013-03-25T02:56:44Z","timestamp":1364180204000},"page":"509-515","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":188,"title":["Whole genome sequencing identifies zoonotic transmission of MRSA isolates with the novel mecA homologue mecC"],"prefix":"10.1038","volume":"5","author":[{"given":"Ewan M.","family":"Harrison","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gavin K.","family":"Paterson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew T.G.","family":"Holden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesper","family":"Larsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Stegger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders Rhod","family":"Larsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Petersen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert L.","family":"Skov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judit Marta","family":"Christensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Bak Zeuthen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ole","family":"Heltberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon R.","family":"Harris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth N.","family":"Zadoks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julian","family":"Parkhill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon J.","family":"Peacock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark A.","family":"Holmes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2013,3,25]]},"reference":[{"key":"BFEMMM201202413_CR1","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1111\/j.1365-2958.2011.07942.x","volume":"83","author":"F Alonzo III","year":"2012","unstructured":"Alonzo F III, Benson MA, Chen J, Novick RP, Shopsin B, Torres VJ (2012) Staphylococcus aureus leucocidin ED contributes to systemic infection by targeting neutrophils and promoting bacterial growth in vivo. Mol Microbiol 83: 423\u2010435","journal-title":"Mol Microbiol"},{"key":"BFEMMM201202413_CR2","doi-asserted-by":"crossref","first-page":"1600","DOI":"10.1016\/j.molimm.2007.10.021","volume":"45","author":"KL Atkins","year":"2008","unstructured":"Atkins KL, Burman JD, Chamberlain ES, Cooper JE, Poutrel B, Bagby S, Jenkins ATA, Feil EJ, van den Elsen JMH (2008) S. aureus IgG\u2010binding proteins SpA and Sbi: Host specificity and mechanisms of immune complex formation. Mol Immunol 45: 1600\u20101611","journal-title":"Mol Immunol"},{"key":"BFEMMM201202413_CR3","doi-asserted-by":"crossref","first-page":"3422","DOI":"10.1093\/bioinformatics\/bti553","volume":"21","author":"TJ Carver","year":"2005","unstructured":"Carver TJ, Rutherford KM, Berriman M, Rajandream MA, Barrell BG, Parkhill J (2005) ACT: the Artemis Comparison Tool. Bioinformatics 21: 3422\u20103423","journal-title":"Bioinformatics"},{"key":"BFEMMM201202413_CR4","doi-asserted-by":"crossref","first-page":"e1002129","DOI":"10.1371\/journal.ppat.1002129","volume":"7","author":"S Castillo\u2010Ramirez","year":"2011","unstructured":"Castillo\u2010Ramirez S, Harris SR, Holden MT, He M, Parkhill J, Bentley SD, Feil EJ (2011) The impact of recombination on dN\/dS within recently emerged bacterial clones. PLoS Pathogens 7: e1002129","journal-title":"PLoS Pathogens"},{"key":"BFEMMM201202413_CR5","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1038\/nrmicro2200","volume":"7","author":"HF Chambers","year":"2009","unstructured":"Chambers HF, Deleo FR (2009) Waves of resistance: Staphylococcus aureus in the antibiotic era. Nat Rev Microbiol 7: 629\u2010641","journal-title":"Nat Rev Microbiol"},{"key":"BFEMMM201202413_CR6","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1126\/science.1198545","volume":"331","author":"NJ Croucher","year":"2011","unstructured":"Croucher NJ, Harris SR, Fraser C, Quail MA, Burton J, van der Linden M, McGee L, von Gottberg A, Song JH, Ko KS, et al (2011) Rapid pneumococcal evolution in response to clinical interventions. Science 331: 430\u2010434","journal-title":"Science"},{"key":"BFEMMM201202413_CR7","doi-asserted-by":"crossref","first-page":"e24360","DOI":"10.1371\/journal.pone.0024360","volume":"6","author":"C Cuny","year":"2011","unstructured":"Cuny C, Layer F, Strommenger B, Witte W (2011) Rare occurrence of methicillin\u2010resistant Staphylococcus aureus CC130 with a novel mecA homologue in humans in Germany. PLoS ONE 6: e24360","journal-title":"PLoS ONE"},{"key":"BFEMMM201202413_CR8","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.1101\/gr.2289704","volume":"14","author":"ACE Darling","year":"2004","unstructured":"Darling ACE, Mau B, Blattner FR, Perna NT (2004) Mauve: multiple alignment of conserved genomic sequence with rearrangements. Genome Res 14: 1394\u20101403","journal-title":"Genome Res"},{"key":"BFEMMM201202413_CR9","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/S8756-3282(01)00632-9","volume":"30","author":"MO Elasri","year":"2002","unstructured":"Elasri MO, Thomas JR, Skinner RA, Blevins JS, Beenken KE, Nelson CL, Smeltzer MS (2002) Staphylococcus aureus collagen adhesin contributes to the pathogenesis of osteomyelitis. Bone 30: 275\u2010280","journal-title":"Bone"},{"key":"BFEMMM201202413_CR10","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1111\/1523-1747.ep12481412","volume":"66","author":"PM Elias","year":"1976","unstructured":"Elias PM, Fritsch P, Mittermayer H (1976) Staphylococcal toxic epidermal necrolysis: species and tissue susceptibility and resistance. J Invest Dermatol 66: 80\u201089","journal-title":"J Invest Dermatol"},{"key":"BFEMMM201202413_CR11","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.tim.2012.01.006","volume":"20","author":"JR Fitzgerald","year":"2012","unstructured":"Fitzgerald JR (2012) Livestock\u2010associated Staphylococcus aureus: origin, evolution and public health threat. Trends Microbiol 20: 192\u2010198","journal-title":"Trends Microbiol"},{"key":"BFEMMM201202413_CR12","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1016\/S1473-3099(11)70126-8","volume":"11","author":"L Garcia\u2010Alvarez","year":"2011","unstructured":"Garcia\u2010Alvarez L, Holden MT, Lindsay H, Webb CR, Brown DF, Curran MD, Walpole E, Brooks K, Pickard DJ, Teale C, et al (2011) Meticillin\u2010resistant Staphylococcus aureus with a novel mecA homologue in human and bovine populations in the UK and Denmark: a descriptive study. Lancet Infect Dis 11: 595\u2010603","journal-title":"Lancet Infect Dis"},{"key":"BFEMMM201202413_CR13","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1093\/gbe\/evq031","volume":"2","author":"CM Guinane","year":"2010","unstructured":"Guinane CM, Ben Zakour NL, Tormo\u2010Mas MA, Weinert LA, Lowder BV, Cartwright RA, Smyth DS, Smyth CJ, Lindsay JA, Gould KA, et al (2010) Evolutionary genomics of Staphylococcus aureus reveals insights into the origin and molecular basis of ruminant host adaptation. Genome Biol Evol 2: 454\u2010466","journal-title":"Genome Biol Evol"},{"key":"BFEMMM201202413_CR14","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1126\/science.1182395","volume":"327","author":"SR Harris","year":"2010","unstructured":"Harris SR, Feil EJ, Holden MT, Quail MA, Nickerson EK, Chantratita N, Gardete S, Tavares A, Day N, Lindsay JA, et al (2010) Evolution of MRSA during hospital transmission and intercontinental spread. Science 327: 469\u2010474","journal-title":"Science"},{"key":"BFEMMM201202413_CR15","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/S1473-3099(12)70268-2","volume":"13","author":"SR Harris","year":"2013","unstructured":"Harris SR, Cartwright EJ, Torok ME, Holden MT, Brown NM, Ogilvy\u2010Stuart AL, Ellington MJ, Quail MA, Bentley SD, Parkhill J, et al (2013) Whole\u2010genome sequencing for analysis of an outbreak of meticillin\u2010resistant Staphylococcus aureus: a descriptive study. Lancet Infect Dis 13: 130\u2010136","journal-title":"Lancet Infect Dis"},{"key":"BFEMMM201202413_CR16","doi-asserted-by":"crossref","first-page":"e1000250","DOI":"10.1371\/journal.ppat.1000250","volume":"4","author":"K Haupt","year":"2008","unstructured":"Haupt K, Reuter M, van den Elsen J, Burman J, H\u00e4lbich S, Richter J, Skerka C, Zipfel PF (2008) The Staphylococcus aureus protein Sbi acts as a complement inhibitor and forms a tripartite complex with host complement factor H and C3b. PLoS Pathogens 4: e1000250","journal-title":"PLoS Pathogens"},{"key":"BFEMMM201202413_CR17","doi-asserted-by":"crossref","first-page":"4997","DOI":"10.1128\/AAC.01199-12","volume":"56","author":"T Ito","year":"2012","unstructured":"Ito T, Hiramatsu K, Tomasz A, de Lencastre H, Perreten V, Holden MT, Coleman DC, Goering R, Giffard PM, Skov RL, et al (2012) Guidelines for reporting novel mecA gene homologues. Antimicrob Agents Chemother 56: 4997\u20104999","journal-title":"Antimicrob Agents Chemother"},{"key":"BFEMMM201202413_CR18","doi-asserted-by":"crossref","first-page":"8352","DOI":"10.1128\/AEM.71.12.8352-8361.2005","volume":"71","author":"HJ Jorgensen","year":"2005","unstructured":"Jorgensen HJ, Mork T, Caugant DA, Kearns A, Rorvik LM (2005) Genetic variation among Staphylococcus aureus strains from Norwegian bulk milk. Appl Environ Microbiol 71: 8352\u20108361","journal-title":"Appl Environ Microbiol"},{"key":"BFEMMM201202413_CR19","doi-asserted-by":"crossref","first-page":"36854","DOI":"10.1074\/jbc.M112.395962","volume":"287","author":"C Kim","year":"2012","unstructured":"Kim C, Milheirico C, Gardete S, Holmes MA, Holden MT, de Lencastre H, Tomasz A (2012) Properties of a novel PBP2A protein homolog from Staphylococcus aureus strain LGA251 and its contribution to the beta\u2010lactam\u2010resistant phenotype. J Biol Chem 287: 36854\u201036863","journal-title":"J Biol Chem"},{"key":"BFEMMM201202413_CR20","doi-asserted-by":"crossref","first-page":"2267","DOI":"10.1056\/NEJMoa1109910","volume":"366","author":"CU Koser","year":"2012","unstructured":"Koser CU, Holden MT, Ellington MJ, Cartwright EJ, Brown NM, Ogilvy\u2010Stuart AL, Hsu LY, Chewapreecha C, Croucher NJ, Harris SR, et al (2012) Rapid whole\u2010genome sequencing for investigation of a neonatal MRSA outbreak. N Engl J Med 366: 2267\u20102275","journal-title":"N Engl J Med"},{"key":"BFEMMM201202413_CR21","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.3201\/eid1809.111920","volume":"18","author":"F Laurent","year":"2012","unstructured":"Laurent F, Chardon H, Haenni M, Bes M, Reverdy ME, Madec JY, Lagier E, Vandenesch F, Tristan A (2012) MRSA harboring mecA variant Gene mecC, France. Emerg Infect Dis 18: 1465\u20101467","journal-title":"Emerg Infect Dis"},{"key":"BFEMMM201202413_CR22","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1017\/S095026881000227X","volume":"139","author":"A Loeffler","year":"2011","unstructured":"Loeffler A, Pfeiffer DU, Lindsay JA, Magalh\u00e3es RJS, Lloyd DH (2011) Prevalence of and risk factors for MRSA carriage in companion animals: a survey of dogs, cats and horses. Epidemiol Infect 139: 1019\u20101028","journal-title":"Epidemiol Infect"},{"key":"BFEMMM201202413_CR23","doi-asserted-by":"crossref","first-page":"19545","DOI":"10.1073\/pnas.0909285106","volume":"106","author":"BV Lowder","year":"2009","unstructured":"Lowder BV, Guinane CM, Ben Zakour NL, Weinert LA, Conway\u2010Morris A, Cartwright RA, Simpson AJ, Rambaut A, Nubel U, Fitzgerald JR (2009) Recent human\u2010to\u2010poultry host jump, adaptation, and pandemic spread of Staphylococcus aureus. Proc Natl Acad Sci USA 106: 19545\u201019550","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFEMMM201202413_CR24","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.jdermsci.2007.05.007","volume":"49","author":"K Nishifuji","year":"2008","unstructured":"Nishifuji K, Sugai M, Amagai M (2008) Staphylococcal exfoliative toxins: \u201cmolecular scissors\u201d of bacteria that attack the cutaneous defense barrier in mammals. J Dermatol Sci 49: 21\u201031","journal-title":"J Dermatol Sci"},{"key":"BFEMMM201202413_CR25","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1111\/j.1462-5822.2012.01832.x","volume":"14","author":"M Otto","year":"2012","unstructured":"Otto M (2012) MRSA virulence and spread. Cell Microbiol 14: 1513\u20101521","journal-title":"Cell Microbiol"},{"key":"BFEMMM201202413_CR26","doi-asserted-by":"crossref","first-page":"127","DOI":"10.3389\/fmicb.2012.00127","volume":"3","author":"A Pantosti","year":"2012","unstructured":"Pantosti A (2012) Methicillin\u2010resistant Staphylococcus aureus associated with animals and its relevance to human health. Frontiers Microbiol 3: 127","journal-title":"Frontiers Microbiol"},{"key":"BFEMMM201202413_CR27","doi-asserted-by":"crossref","first-page":"2809","DOI":"10.1093\/jac\/dks329","volume":"67","author":"GK Paterson","year":"2012","unstructured":"Paterson GK, Larsen AR, Robb A, Edwards GE, Pennycott TW, Foster G, Mot D, Hermans K, Baert K, Peacock SJ, et al (2012) The newly described mecA homologue, mecALGA251, is present in methicillin\u2010resistant Staphylococcus aureus isolates from a diverse range of host species. J Antimicrob Chemother 67: 2809\u20102813","journal-title":"J Antimicrob Chemother"},{"key":"BFEMMM201202413_CR28","doi-asserted-by":"crossref","first-page":"E16","DOI":"10.1111\/1469-0691.12036","volume":"19","author":"A Petersen","year":"2013","unstructured":"Petersen A, Stegger M, Heltberg O, Christensen J, Zeuthen A, Knudsen LK, Urth T, Sorum M, Schouls L, Larsen J, et al (2013) Epidemiology of methicillin\u2010resistant Staphylococcus aureus carrying the novel mecC gene in Denmark corroborates a zoonotic reservoir with transmission to humans. Clin Microbiol Infect 19: E16\u2010E22","journal-title":"Clin Microbiol Infect"},{"key":"BFEMMM201202413_CR29","doi-asserted-by":"publisher","unstructured":"Price LB, Stegger M, Hasman H, Aziz M, Larsen J, Andersen PS, Pearson T, Waters AE, Foster JT, Schupp J et al (2012) Staphylococcus aureus CC398: host adaptation and emergence of methicillin resistance in livestock. MBio 3, DOI: https:\/\/doi.org\/10.1128\/mBio.00305\u201011","DOI":"10.1128\/mBio.00305-11"},{"key":"BFEMMM201202413_CR30","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1038\/nmeth.1270","volume":"5","author":"MA Quail","year":"2008","unstructured":"Quail MA, Kozarewa I, Smith F, Scally A, Stephens PJ, Durbin R, Swerdlow H, Turner DJ (2008) A large genome center's improvements to the Illumina sequencing system. Nat Methods 5: 1005\u20101010","journal-title":"Nat Methods"},{"key":"BFEMMM201202413_CR31","doi-asserted-by":"crossref","first-page":"3776","DOI":"10.1128\/IAI.68.6.3776-3779.2000","volume":"68","author":"MN Rhem","year":"2000","unstructured":"Rhem MN, Lech EM, Patti JM, McDevitt D, Hook M, Jones DB, Wilhelmus KR (2000) The collagen\u2010binding adhesin is a virulence factor in Staphylococcus aureus keratitis. Infect Immun 68: 3776\u20103779","journal-title":"Infect Immun"},{"key":"BFEMMM201202413_CR32","doi-asserted-by":"crossref","first-page":"3765","DOI":"10.1128\/AAC.00187-11","volume":"55","author":"AC Shore","year":"2011","unstructured":"Shore AC, Deasy EC, Slickers P, Brennan G, O'Connell B, Monecke S, Ehricht R, Coleman DC (2011) Detection of staphylococcal cassette chromosome mec type XI carrying highly divergent mecA, mecI,mecR1, blaZ, and ccr genes in human clinical isolates of clonal complex 130 methicillin\u2010resistant Staphylococcus aureus. Antimicrob Agents Chemother 55: 3765\u20103773","journal-title":"Antimicrob Agents Chemother"},{"key":"BFEMMM201202413_CR33","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1093\/bioinformatics\/bti191","volume":"21","author":"A Stamatakis","year":"2005","unstructured":"Stamatakis A, Ludwig T, Meier H (2005) RAxML\u2010III: a fast program for maximum likelihood\u2010based inference of large phylogenetic trees. Bioinformatics 21: 456\u2010463","journal-title":"Bioinformatics"},{"key":"BFEMMM201202413_CR34","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1111\/j.1469-0691.2011.03715.x","volume":"18","author":"M Stegger","year":"2012","unstructured":"Stegger M, Andersen PS, Kearns A, Pichon B, Holmes MA, Edwards G, Laurent F, Teale C, Skov R, Larsen AR (2012) Rapid detection, differentiation and typing of methicillin\u2010resistant Staphylococcus aureus harbouring either mecA or the new mecA homologue mecA(LGA251). Clin Microbiol Infect 18: 395\u2010400","journal-title":"Clin Microbiol Infect"},{"key":"BFEMMM201202413_CR35","doi-asserted-by":"crossref","first-page":"6207","DOI":"10.1128\/JB.05847-11","volume":"193","author":"QC Truong\u2010Bolduc","year":"2011","unstructured":"Truong\u2010Bolduc QC, Dunman PM, Eidem T, Hooper DC (2011) Transcriptional profiling analysis of the global regulator NorG, a GntR\u2010like protein of Staphylococcus aureus. J Bacteriol 193: 6207\u20106214","journal-title":"J Bacteriol"},{"key":"BFEMMM201202413_CR36","doi-asserted-by":"crossref","first-page":"4550","DOI":"10.1073\/pnas.1113219109","volume":"109","author":"BC Young","year":"2012","unstructured":"Young BC, Golubchik T, Batty EM, Fung R, Larner\u2010Svensson H, Votintseva AA, Miller RR, Godwin H, Knox K, Everitt RG, et al (2012) Evolutionary dynamics of Staphylococcus aureus during progression from carriage to disease. Proc Natl Acad Sci USA 109: 4550\u20104555","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFEMMM201202413_CR37","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1101\/gr.074492.107","volume":"18","author":"DR Zerbino","year":"2008","unstructured":"Zerbino DR, Birney E (2008) Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res 18: 821\u2010829","journal-title":"Genome Res"}],"container-title":["EMBO Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1002\/emmm.201202413.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/emmm.201202413","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1002\/emmm.201202413\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.embopress.org\/doi\/pdf\/10.1002\/emmm.201202413","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1002\/emmm.201202413.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T06:05:49Z","timestamp":1779084349000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1002\/emmm.201202413"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,3,25]]},"references-count":37,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,4,1]]}},"alternative-id":["BFEMMM201202413"],"URL":"https:\/\/doi.org\/10.1002\/emmm.201202413","relation":{},"ISSN":["1757-4676","1757-4684"],"issn-type":[{"value":"1757-4676","type":"print"},{"value":"1757-4684","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,3,25]]},"assertion":[{"value":"21 December 2012","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 February 2013","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 February 2013","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 March 2013","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}]}}