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The two BTFs, hereby designated as \u201cBTF\u2010A\u201d and \u201cBTF\u2010B,\u201d were run parallel and used ethanol as co\u2010metabolite at different loading rates (LRs), and a lipopeptide\u2010type biosurfactant that was generated by the gram\u2010positive bacteria, Surfactin, respectively. The results using BTF\u2010A showed that adding ethanol at a higher rate of 4.59\u00a0g\/(m<jats:sup>3<\/jats:sup>\u00a0h) resulted in removal efficiencies of 85% and 87% for CF and DCBM, respectively. Conversely, for the same LR, the use of Surfactin without ethanol (BTF\u2010B) showed comparable removal efficiencies of 85% and 80% for CF and DCBM, respectively. The maximum rate constant for CF and DCBM for the BTF\u2010A was 0.00203\u00a0s<jats:sup>\u22121<\/jats:sup> and 0.0022\u00a0s<jats:sup>\u22121<\/jats:sup>, respectively. For the same THMs LR, similar reaction rate constants resulted for the BTF\u2010B. Further studies were conducted to investigate and understand the microbial diversity within both BTFs. The result indicated that for BTF with co\u2010metabolite, <jats:italic>Fusarium sp<\/jats:italic>. was the most dominant fungi over 98% followed by <jats:italic>F. Solani<\/jats:italic> with less than 2%. <jats:italic>F. oxysporum<\/jats:italic> and <jats:italic>Fusarium sp<\/jats:italic>. were instead the dominant fungi for the BTF with Surfactin. Before introducing the Surfactin into the BTF, the batch experiment was conducted to evaluate the effectiveness of synthetic surfactant as compared to a biosurfactant (Surfactin). In this regard, vials with Surfactin showed better performance than vials with Tomadol 25\u20107 (synthetic surfactant).<\/jats:p>","DOI":"10.1002\/eng2.12031","type":"journal-article","created":{"date-parts":[[2019,8,16]],"date-time":"2019-08-16T12:50:37Z","timestamp":1565959837000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Effectiveness of biosurfactant for the removal of trihalomethanes by biotrickling filter"],"prefix":"10.1002","volume":"1","author":[{"given":"Bineyam","family":"Mezgebe","sequence":"first","affiliation":[{"name":"Department of Chemical and Environmental Engineering College of Engineering and Applied Science, University of Cincinnati  Cincinnati Ohio"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1985-0745","authenticated-orcid":false,"given":"George","family":"Sorial","sequence":"additional","affiliation":[{"name":"Department of Chemical and Environmental Engineering College of Engineering and Applied Science, University of Cincinnati  Cincinnati Ohio"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Wendell","sequence":"additional","affiliation":[{"name":"Department of Chemical and Environmental Engineering College of Engineering and Applied Science, University of Cincinnati  Cincinnati Ohio"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Sahle\u2010Demessie","sequence":"additional","affiliation":[{"name":"National Risk Management Research Laboratory, Office of Research and Development US Environmental Protection Agency  Cincinnati Ohio"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,8,16]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsta.2009.0108"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.2166\/wh.2007.013"},{"key":"e_1_2_8_4_1","unstructured":"USEPA.TRI national analysis: Report sections for the TRI national analysis.2019. 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