{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T12:45:19Z","timestamp":1786193119204,"version":"build-2736575974"},"reference-count":38,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T00:00:00Z","timestamp":1786147200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T00:00:00Z","timestamp":1786147200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Epileptic Disorders"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study aims to investigate the expression pattern of long noncoding RNA SNHG16 in pediatric temporal lobe epilepsy (TLE), its clinical diagnostic value, and its molecular mechanisms in epilepsy\u2010related neuronal damage.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study included 78 newly diagnosed pediatric TLE patients and 75 healthy control children. SNHG16 expression was detected via RT\u2010qPCR, and its diagnostic efficacy was evaluated using ROC curves. In\u00a0vitro, a TLE model was established in human hippocampal neuronal cells treated with magnesium\u2010free medium. Cell viability, apoptosis, inflammatory factor levels (IL\u20106, IL\u20101\u03b2, TNF\u2010\u03b1), and oxidative stress markers (SOD, GSH, MDA) were assessed using MTT assay, flow cytometry, ELISA, and biochemical kits, respectively. The targeting relationship between SNHG16 and miR\u2010485\u20105p was validated through dual\u2010luciferase reporter assays and RIP experiments.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Serum SNHG16 expression was significantly upregulated in TLE pediatric patients, with an area under the curve (AUC) of 0.895. In cell models, silencing SNHG16 significantly alleviated magnesium\u2010deprivation\u2010induced decreases in cell viability, increased apoptosis, oxidative stress, and inflammatory responses. Mechanistically, SNHG16 directly binds and negatively regulates miR\u2010485\u20105p expression in the cytoplasm. Inhibiting miR\u2010485\u20105p reversed the neuroprotective effects induced by SNHG16 knockdown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>SNHG16 is highly expressed in pediatric TLE and has certain diagnostic potential. It exacerbates epilepsy\u2010related neuronal damage by binding to and suppressing miR\u2010485\u20105p function. This study provides novel insights into understanding the disease mechanism.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/epd2.70316","type":"journal-article","created":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T11:55:27Z","timestamp":1786190127000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The\n                    <scp>SNHG16<\/scp>\n                    \/\n                    <scp>miR<\/scp>\n                    \u2010485\u20105p axis exacerbates neuronal injury in pediatric temporal lobe epilepsy"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-5237-2620","authenticated-orcid":false,"given":"Xuemin","family":"Ding","sequence":"first","affiliation":[{"name":"Department of Children's Medical Center Changde Hospital, Xiangya School of Medicine, Central South University (The first people's hospital of Changde city)  Changde China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-8234-6092","authenticated-orcid":false,"given":"Haixia","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Changde Hospital, Xiangya School of Medicine, Central South University (The first people\u2019s hospital of Changde city)  Changde China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,8]]},"reference":[{"issue":"10503","key":"e_1_2_9_2_1","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1016\/S0140-6736(25)00773-1","article-title":"Childhood epilepsy","volume":"406","author":"Menon RN","year":"2025","journal-title":"Lancet"},{"issue":"5","key":"e_1_2_9_3_1","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1002\/epd2.70057","article-title":"Electrophysiological and clinical markers of SUDEP risk in pediatric epilepsy","volume":"27","author":"Ozbudak 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