{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T19:58:19Z","timestamp":1775678299350,"version":"3.50.1"},"reference-count":14,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T00:00:00Z","timestamp":1699401600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R01 NS115831"],"award-info":[{"award-number":["R01 NS115831"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R21 NS127064"],"award-info":[{"award-number":["R21 NS127064"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["CA268634"],"award-info":[{"award-number":["CA268634"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Epilepsia Open"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Low\u2010grade epilepsy\u2010associated tumors (LEATs) are a common cause of drug\u2010resistant epilepsy in children. Herein, we demonstrate the feasibility of using tumor tissue derived from stereoelectroencephalography (sEEG) electrodes upon removal to molecularly characterize tumors and aid in diagnosis. An 18\u2010year\u2010old male with focal epilepsy and MRI suggestive of a dysembryoplastic neuroepithelial tumor (DNET) in the left posterior temporal lobe underwent implantation of seven peri\u2010tumoral sEEG electrodes for peri\u2010operative language mapping and demarcation of the peri\u2010tumoral ictal zone prior to DNET resection. Using electrodes that passed through tumor tissue, we show successful isolation of tumor DNA and subsequent analysis using standard methods for tumor classification by DNA, including Glioseq targeted sequencing and DNA methylation array analysis. This study provides preliminary evidence for the feasibility of molecular diagnosis of LEATs or other lesions using a minimally invasive method with microscopic tissue volumes. The implications of sEEG electrodes in tumor characterization are broad but would aid in diagnosis and subsequent targeted therapeutic strategies.<\/jats:p>","DOI":"10.1002\/epi4.12840","type":"journal-article","created":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T05:33:44Z","timestamp":1696570424000},"page":"409-416","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Characterization of low\u2010grade epilepsy\u2010associated tumor from implanted stereoelectroencephalography electrodes"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7456-7996","authenticated-orcid":false,"given":"Taylor A.","family":"Gatesman","sequence":"first","affiliation":[{"name":"Department of Neurological Surgery University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"},{"name":"Department of Cellular and Molecular Pathology University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"},{"name":"John G. Rangos Sr. Research Center Children's Hospital of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jasmine L.","family":"Hect","sequence":"additional","affiliation":[{"name":"Department of Neurological Surgery University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"},{"name":"John G. Rangos Sr. Research Center Children's Hospital of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0512-4590","authenticated-orcid":false,"given":"H. Westley","family":"Phillips","sequence":"additional","affiliation":[{"name":"Department of Neurological Surgery University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brenden J.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Neurological Surgery University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"},{"name":"John G. Rangos Sr. Research Center Children's Hospital of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abigail I.","family":"Wald","sequence":"additional","affiliation":[{"name":"Molecular and Genomic Pathology University of Pittsburgh Medical Center Health System  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colleen","family":"McClung","sequence":"additional","affiliation":[{"name":"Department of Psychiatry University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina N.","family":"Nikiforova","sequence":"additional","affiliation":[{"name":"Molecular and Genomic Pathology University of Pittsburgh Medical Center Health System  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John M.","family":"Skaugen","sequence":"additional","affiliation":[{"name":"Molecular and Genomic Pathology University of Pittsburgh Medical Center Health System  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian F.","family":"Pollack","sequence":"additional","affiliation":[{"name":"Department of Neurological Surgery University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"},{"name":"John G. Rangos Sr. Research Center Children's Hospital of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5089-460X","authenticated-orcid":false,"given":"Taylor J.","family":"Abel","sequence":"additional","affiliation":[{"name":"Department of Neurological Surgery University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"},{"name":"Department of Psychiatry University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sameer","family":"Agnihotri","sequence":"additional","affiliation":[{"name":"Department of Neurological Surgery University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"},{"name":"Department of Cellular and Molecular Pathology University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"},{"name":"John G. Rangos Sr. Research Center Children's Hospital of Pittsburgh  Pittsburgh Pennsylvania USA"},{"name":"Department of Neurobiology University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,11,8]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2016.173"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10143\u2010014\u20100527\u20109"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/epi.16328"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000201469"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/braincomms\/fcad174"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.3171\/2018.1.Peds17435"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/neuonc\/nov289"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/nlz055"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature26000"},{"key":"e_1_2_13_11_1","unstructured":"conumee: Enhanced copy\u2010number variation analysis using Illumina DNA methylation arrays.2017."},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1754"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/neuonc\/nov289"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/nlz055"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13148\u2010019\u20100766\u20102"}],"container-title":["Epilepsia Open"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/epi4.12840","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T06:47:50Z","timestamp":1707288470000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/epi4.12840"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,8]]},"references-count":14,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["10.1002\/epi4.12840"],"URL":"https:\/\/doi.org\/10.1002\/epi4.12840","archive":["Portico"],"relation":{},"ISSN":["2470-9239","2470-9239"],"issn-type":[{"value":"2470-9239","type":"print"},{"value":"2470-9239","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,8]]},"assertion":[{"value":"2023-04-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}