{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T07:05:29Z","timestamp":1778655929234,"version":"3.51.4"},"reference-count":23,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2013,8,24]],"date-time":"2013-08-24T00:00:00Z","timestamp":1377302400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2013,10,22]],"date-time":"2013-10-22T00:00:00Z","timestamp":1382400000000},"content-version":"vor","delay-in-days":59,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Fundo Europeu de Desenvolvimento Regional (FEDER"},{"name":"Programa Operacional Factores de Competitividade"},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia"},{"name":"FUBIA","award":["FCOMP-01-0124-FEDER-008651"],"award-info":[{"award-number":["FCOMP-01-0124-FEDER-008651"]}]},{"name":"FUBIA","award":["PTDC\/AAC-CLI\/103719\/2008"],"award-info":[{"award-number":["PTDC\/AAC-CLI\/103719\/2008"]}]},{"name":"Sara Novais","award":["SFRH\/BPD\/80337\/2011"],"award-info":[{"award-number":["SFRH\/BPD\/80337\/2011"]}]}],"content-domain":{"domain":["setac.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2013,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>\u2003<\/jats:title>\n                  <jats:p>Cellular energy allocation (CEA) is a methodology developed to evaluate the effects of toxic stress on the metabolic balance of organisms. It consists of the integration of the energy reserves available (Ea; total carbohydrate, protein and lipid content) and energy consumption (Ec) estimated by measuring electron transport system (ETS) activity. The main goal of the present study was to evaluate the effects on the energy budget of the soil invertebrate Enchytraeus albidus (Oligochaeta) after exposure to dimethoate, atrazine, and carbendazim (by testing the reproduction 10% effective concentration, 20% effective concentration, 50% effective concentration and 90% effective concentration) over periods of time from 0 d to 2 d, 4 d, and 8 d. Significant changes in energy reserves were observed with all pesticides, together with effects on energy consumption. Carbohydrates were the first energy source to be used, and clear depletions occurred with all pesticides. Energy consumption increased generally over longer exposures and with higher concentrations of the pesticides. Although clear changes were seen in the individual energy reserve budgets and on Ec, CEA was only significantly reduced with atrazine exposures longer than 4 d. The nearly absent effects on CEA at concentrations known to affect reproduction indicate that the reduction in reproduction is not likely to be caused by a reduction in the total energy budget during the first 8 d of exposure. The present study showed the importance of complementing CEA interpretation with the individual Ea and Ec parameters, in particular if these show opposite balances. The Ea and Ec results were in good agreement with gene transcription results from a parallel study, hence suggesting translation and showing the advantage of combining various effect levels to advance the understanding of mechanisms. Environ Toxicol Chem 2013;32:2800\u20132807. \u00a9 2013 SETAC<\/jats:p>\n               <\/jats:sec>","DOI":"10.1002\/etc.2368","type":"journal-article","created":{"date-parts":[[2013,8,24]],"date-time":"2013-08-24T11:18:36Z","timestamp":1377343116000},"page":"2800-2807","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Changes in cellular energy allocation in <i>Enchytraeus albidus<\/i> when exposed to dimethoate, atrazine, and carbendazim"],"prefix":"10.1093","volume":"32","author":[{"given":"Sara C.","family":"Novais","sequence":"first","affiliation":[{"name":"Department of Biology and Center for Environmental and Marine Studies (CESAM)University of Aveiro, , Aveiro , Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00f3nica J.B.","family":"Amorim","sequence":"additional","affiliation":[{"name":"Department of Biology and Center for Environmental and Marine Studies (CESAM)University of Aveiro, , Aveiro , Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2013,8,24]]},"reference":[{"key":"2025010314183051700_etc2368-bib-0001","first-page":"1632","article-title":"The missing biomarker link: Relationships between effects on the cellular energy allocation biomarker of toxicant-stressed Daphnia magna and corresponding population characteristics","volume":"22","author":"De Coen","year":"2003","journal-title":"Environ Toxicol Chem"},{"key":"2025010314183051700_etc2368-bib-0002","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1023\/A:1008228517955","article-title":"The use of biomarkers in Daphnia magna toxicity testing. IV. Cellular Energy Allocation: A new methodology to assess the energy budget of toxicant-stressed Daphnia populations","volume":"6","author":"De Coen","year":"1997","journal-title":"J Aquat Ecosyst Stress Recovery"},{"key":"2025010314183051700_etc2368-bib-0003","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/j.ecoenv.2006.12.017","article-title":"Comparative studies on the uptake and effects of cadmium and zinc on the cellular energy allocation of two freshwater gastropods","volume":"68","author":"Moolman","year":"2007","journal-title":"Ecotoxicol Environ Saf"},{"key":"2025010314183051700_etc2368-bib-0004","doi-asserted-by":"crossref","first-page":"283","DOI":"10.2307\/2389587","article-title":"A physiological-basis of population processes\u2014Ecotoxicological implications","volume":"4","author":"Calow","year":"1990","journal-title":"Funct Ecol"},{"key":"2025010314183051700_etc2368-bib-0005","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jembe.2003.12.022","article-title":"Cellular energy allocation and scope for growth in the estuarine mysid Neomysis integer (Crustacea:Mysidacea) following chlorpyrifos exposure: A method comparison","volume":"306","author":"Verslycke","year":"2004","journal-title":"J Exp Mar Biol Ecol"},{"key":"2025010314183051700_etc2368-bib-0006","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S0022-0981(03)00006-6","article-title":"Cellular energy allocation in the estuarine mysid shrimp Neomysis integer (Crustacea:Mysidacea) following tributyltin exposure","volume":"288","author":"Verslycke","year":"2003","journal-title":"J Exp Mar Biol Ecol"},{"key":"2025010314183051700_etc2368-bib-0007","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.jembe.2008.01.015","article-title":"Metallothionein and cellular energy allocation in the estuarine mysid shrimp Neomysis integer exposed to cadmium at different salinities","volume":"357","author":"Erk","year":"2008","journal-title":"J Exp Mar Biol Ecol"},{"key":"2025010314183051700_etc2368-bib-0008","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.envpol.2003.09.027","article-title":"Cellular energy allocation in zebra mussels exposed along a pollution gradient: Linking cellular effects to higher levels of biological organization","volume":"129","author":"Smolders","year":"2004","journal-title":"Environ Pollut"},{"key":"2025010314183051700_etc2368-bib-0009","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1080\/15287390802539178","article-title":"Cellular energy allocation in Hediste diversicolor exposed to sediment contaminants","volume":"72","author":"Stomperudhaugen","year":"2009","journal-title":"J Toxicol Environ Health A"},{"key":"2025010314183051700_etc2368-bib-0010","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1016\/j.chemosphere.2012.09.030","article-title":"Exposure of Enchytraeus albidus to Cd and Zn\u2014Changes in cellular energy allocation (CEA) and linkage to transcriptional, enzymatic and reproductive effects","volume":"90","author":"Novais","year":"2013","journal-title":"Chemosphere"},{"key":"2025010314183051700_etc2368-bib-0011","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1007\/s11270-011-0867-9","article-title":"Energy basal levels and allocation among lipids, proteins, and carbohydrate in Enchytraeus albidus: Changes related to exposure to Cu-salt and Cu-nanoparticles","volume":"223","author":"Amorim","year":"2012","journal-title":"Water Air Soil Pollut"},{"key":"2025010314183051700_etc2368-bib-0012","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.chemosphere.2010.01.011","article-title":"Can avoidance in Enchytraeus albidus be used as a screening parameter for pesticides testing","volume":"79","author":"Novais","year":"2010","journal-title":"Chemosphere"},{"key":"2025010314183051700_etc2368-bib-0013","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/j.envint.2007.08.010","article-title":"Enchytraeus albidus (Enchytraeidae): A test organism in a standardised avoidance test? Effects of different chemical substances","volume":"34","author":"Amorim","year":"2008","journal-title":"Environ Int"},{"key":"2025010314183051700_etc2368-bib-0014","doi-asserted-by":"crossref","first-page":"e36068","DOI":"10.1371\/journal.pone.0036068","article-title":"Gene expression responses linked to reproduction effect concentrations (EC10,20,50,90) of dimethoate, atrazine and carbendazim, in Enchytraeus albidus","volume":"7","author":"Novais","year":"2012","journal-title":"PLoS One"},{"key":"2025010314183051700_etc2368-bib-0015","first-page":"74","article-title":"Ueber Enchytraeus, eine neue Anneliden-Gattung","author":"Henle","year":"1837","journal-title":"Arch Anat Physiol Med"},{"key":"2025010314183051700_etc2368-bib-0016","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"Rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal Biochem"},{"key":"2025010314183051700_etc2368-bib-0017","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1139\/y59-099","article-title":"A rapid method of total lipid extraction and purification","volume":"37","author":"Bligh","year":"1959","journal-title":"Can J Biochem Physiol"},{"key":"2025010314183051700_etc2368-bib-0018","doi-asserted-by":"crossref","first-page":"849","DOI":"10.4319\/lo.1975.20.5.0849","article-title":"Respiration and the activity of the respiratory electron transport system in marine zooplankton","volume":"20","author":"King","year":"1975","journal-title":"Limnol Oceanogr"},{"key":"2025010314183051700_etc2368-bib-0019","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1007\/978-3-642-81863-9_30","volume-title":"Polarographic Oxygen Sensors: Aquatic and Physiological Applications","author":"Gnaiger","year":"1983"},{"key":"2025010314183051700_etc2368-bib-0020","volume-title":"SigmaPlot for Windows","author":"SigmaPlot","year":"1997"},{"key":"2025010314183051700_etc2368-bib-0021","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1002\/etc.5620220429","article-title":"Changes in cellular energy budget as a measure of whole effluent toxicity in zebrafish (Danio rerio)","volume":"22","author":"Smolders","year":"2003","journal-title":"Environ Toxicol Chem"},{"key":"2025010314183051700_etc2368-bib-0022","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.aquatox.2008.12.012","article-title":"Nickel and binary metal mixture responses in Daphnia magna: Molecular fingerprints and (sub)organismal effects","volume":"92","author":"Vandenbrouck","year":"2009","journal-title":"Aquat Toxicol"},{"key":"2025010314183051700_etc2368-bib-0023","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.aquatox.2007.04.010","article-title":"Molecular responses during cadmium-induced stress in Daphnia magna: Integration of differential gene expression with higher-level effects","volume":"83","author":"Soetaert","year":"2007","journal-title":"Aquat Toxicol"}],"container-title":["Environmental Toxicology and Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fetc.2368","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/etc\/article-pdf\/32\/12\/2800\/60123446\/etc2368.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/etc\/article-pdf\/32\/12\/2800\/60123446\/etc2368.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,3]],"date-time":"2025-01-03T14:58:58Z","timestamp":1735916338000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/etc\/article\/32\/12\/2800\/7736346"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8,24]]},"references-count":23,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2013,8,24]]},"published-print":{"date-parts":[[2013,12,1]]}},"URL":"https:\/\/doi.org\/10.1002\/etc.2368","relation":{},"ISSN":["0730-7268","1552-8618"],"issn-type":[{"value":"0730-7268","type":"print"},{"value":"1552-8618","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2013,12,1]]},"published":{"date-parts":[[2013,8,24]]},"assertion":[{"value":"2013-03-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-08-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-10-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}