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Acute toxicity tests of silver (Ag), zinc oxide (ZnO), and titanium dioxide (TiO2) ENPs with Daphnia magna were carried out following Organisation for Economic Co-operation and Development test guidelines. Daphnia magna was found to be very sensitive to Ag ENPs (48-h 50% effective concentration 33\u2009\u03bcg\u2009L\u22121), and aging of the test suspensions in M7 medium (up to 48\u2009h) did not decrease toxicity significantly. Conversely, the presence of Suwannee River natural organic matter (NOM; 20\u2009mg\u2009L\u22121) completely alleviated Ag ENP toxicity in all testing scenarios and did not aid in stabilizing suspensions. In contrast, addition of Suwannee River NOM stabilized ZnO ENP suspensions and did not decrease toxicity. Aging for 48\u2009h generated monotonous concentration\u2013response curves in the presence and absence of Suwannee River NOM. At concentrations up to 100\u2009mg\u2009L\u22121 TiO2 ENPs did not cause immobilization of D. magna under any of the tested conditions. Presence of Suwannee River NOM caused agglomeration in stock suspensions. The authors' results suggest that aging and presence of Suwannee River NOM are important parameters in standard toxicity testing of ENPs, which in some cases may aid in gaining better control over the exposure conditions but in other cases might contribute to agglomeration or elimination of ENP toxicity. Therefore, modifications to the current guidelines for testing ENPs should be evaluated on a case-by-case basis. Environ Toxicol Chem 2015;34:497\u2013506. \u00a9 2014 SETAC<\/jats:p>\n               <\/jats:sec>","DOI":"10.1002\/etc.2855","type":"journal-article","created":{"date-parts":[[2014,12,26]],"date-time":"2014-12-26T11:39:48Z","timestamp":1419593988000},"page":"497-506","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":110,"title":["The influence of natural organic matter and aging on suspension stability in guideline toxicity testing of silver, zinc oxide, and titanium dioxide nanoparticles with <i>Daphnia magna<\/i>"],"prefix":"10.1093","volume":"34","author":[{"given":"Denisa","family":"Cupi","sequence":"first","affiliation":[{"name":"Department of Environmental EngineeringTechnical University of Denmark Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nanna B.","family":"Hartmann","sequence":"additional","affiliation":[{"name":"Department of Environmental EngineeringTechnical University of Denmark Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Baun","sequence":"additional","affiliation":[{"name":"Department of Environmental EngineeringTechnical University of Denmark Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2014,12,26]]},"reference":[{"key":"2025010315532624300_etc2855-bib-0001","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1002\/etc.726","article-title":"Potential scenarios for nanomaterial release and subsequent alteration in the environment","volume":"31","author":"Nowack","year":"2012","journal-title":"Environ Toxicol Chem"},{"key":"2025010315532624300_etc2855-bib-0002","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.envint.2012.11.009","article-title":"Minimum physicochemical characterisation requirements for nanomaterial regulation","volume":"52","author":"Pettitt","year":"2013","journal-title":"Environ 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