{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T22:52:52Z","timestamp":1791413572736,"version":"4.3.3"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2015,7,2]],"date-time":"2015-07-02T00:00:00Z","timestamp":1435795200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2015,12,9]],"date-time":"2015-12-09T00:00:00Z","timestamp":1449619200000},"content-version":"vor","delay-in-days":160,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Environmental Health Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["setac.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2015,7,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>\u2003<\/jats:title>\n                  <jats:p>Estrogenic endocrine-disrupting chemicals are found in environmental and biological samples, commercial and consumer products, food, and numerous other sources. Given their ubiquitous nature and potential for adverse effects, a critical need exists for rapidly detecting these chemicals. The authors developed an estrogen-responsive recombinant human ovarian (BG1Luc4E2) cell line recently accepted by the US Environmental Protection Agency (USEPA) and Organisation for Economic Co-operation and Development (OECD) as a bioanalytical method to detect estrogen receptor (ER) agonists\/antagonists. Unfortunately, these cells appear to contain only 1 of the 2 known ER isoforms, ER\u03b1 but not ER\u03b2, and the differential ligand selectivity of these ERs indicates that the currently accepted screening method only detects a subset of total estrogenic chemicals. To improve the estrogen screening bioassay, BG1Luc4E2 cells were stably transfected with an ER\u03b2 expression plasmid and positive clones identified using ER\u03b2-selective ligands (genistein and Br-ER\u03b2-041). A highly responsive clone (BG1LucER\u03b2c9) was identified that exhibited greater sensitivity and responsiveness to ER\u03b2-selective ligands than BG1Luc4E2 cells, and quantitative reverse-transcription polymerase chain reaction confirmed the presence of ER\u03b2 expression in these cells. Screening of pesticides and industrial chemicals identified chemicals that preferentially stimulated ER\u03b2-dependent reporter gene expression. Together, these results not only demonstrate the utility of this dual-ER recombinant cell line for detecting a broader range of estrogenic chemicals than the current BG1Luc4E2 cell line, but screening with both cell lines allows identification of ER\u03b1- and ER\u03b2-selective chemicals. Environ Toxicol Chem 2016;35:91\u2013100. \u00a9 2015 SETAC<\/jats:p>\n               <\/jats:sec>","DOI":"10.1002\/etc.3146","type":"journal-article","created":{"date-parts":[[2015,7,3]],"date-time":"2015-07-03T15:58:03Z","timestamp":1435939083000},"page":"91-100","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Development of a recombinant human ovarian (BG1) cell line containing estrogen receptor \u03b1 and \u03b2 for improved detection of estrogenic\/antiestrogenic chemicals"],"prefix":"10.1093","volume":"35","author":[{"given":"Jennifer C.","family":"Brennan","sequence":"first","affiliation":[{"name":"Department of Environmental ToxicologyUniversity of California Davis California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arzoo","family":"Bassal","sequence":"additional","affiliation":[{"name":"Department of Environmental ToxicologyUniversity of California Davis California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guochun","family":"He","sequence":"additional","affiliation":[{"name":"Department of Environmental ToxicologyUniversity of California Davis California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael S.","family":"Denison","sequence":"additional","affiliation":[{"name":"Department of Environmental ToxicologyUniversity of California Davis California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2015,7,2]]},"reference":[{"key":"2025010315234635800_etc3146-bib-0001","author":"Bergman","year":"2013"},{"key":"2025010315234635800_etc3146-bib-0002","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1080\/10408449891344236","article-title":"Endocrine disruption in wildlife: A critical review of the evidence","volume":"28","author":"Tyler","year":"1998","journal-title":"Crit Rev Toxicol"},{"key":"2025010315234635800_etc3146-bib-0003","first-page":"67","article-title":"Recombinant cell bioassays for endocrine disruptors: Development of a stably transfected human ovarian cell line for the detection of estrogenic and anti-estrogenic chemicals","volume":"13","author":"Rogers","year":"2000","journal-title":"In Vitro Mol Toxicol"},{"key":"2025010315234635800_etc3146-bib-0004","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.steroids.2014.08.004","article-title":"Is there a role for estrogen activity assays? 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