{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T09:14:01Z","timestamp":1758705241197,"version":"3.32.0"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2015,7,25]],"date-time":"2015-07-25T00:00:00Z","timestamp":1437782400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2015,12,3]],"date-time":"2015-12-03T00:00:00Z","timestamp":1449100800000},"content-version":"vor","delay-in-days":131,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["setac.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2015,7,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>\u2003<\/jats:title>\n                  <jats:p>In Japan, understanding the environmental persistence of chemicals is very important for risk assessment, and ready biodegradability tests are mainly conducted according to the Organisation for Economic Co-operation and Development test guideline 301C. However, the highest test concentration specified in test guideline 301C, 100\u2009mg\/L, may cause microbial toxicity and incomplete biodegradation. The authors performed test guideline 301C tests at test concentrations of 30\u2009mg\/L for 13 substances that were readily biodegradable in ready biodegradability tests but not in test guideline 301C tests. Of the 5 substances with potential to cause microbial toxicity at 100\u2009mg\/L, the percentage of biodegradation of sodium dimethyldithiocarbamate, 4-chloro-3-cresol (CC), thymol (THY), and p-tert-butyl-\u03b1-methylbenzenepropionaldehyde measured by biochemical oxygen demand (BOD) increased in the test guideline 301C test at 30\u2009mg\/L, suggesting a reduction in toxicity effects. Furthermore, CC and THY met the criteria for ready biodegradability, which are more than 60% of biodegradation by BOD and a 10-d window. Of the 8 substances with a low potential for causing microbial toxicity at 100\u2009mg\/L, the percentage of biodegradation of only 2-(diethylamino)ethanol increased in the test guideline 301C test at 30\u2009mg\/L. Employing a lower test concentration in the standard test guideline 301C test will contribute to improvement of consistency between results of a test guideline 301C test and other ready biodegradability tests. Environ Toxicol Chem 2016;35:84\u201390. \u00a9 2015 SETAC<\/jats:p>\n               <\/jats:sec>","DOI":"10.1002\/etc.3180","type":"journal-article","created":{"date-parts":[[2015,7,25]],"date-time":"2015-07-25T23:07:40Z","timestamp":1437865660000},"page":"84-90","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Effect of test concentration in the ready biodegradability test for chemical substances: Improvement of OECD test guideline 301C"],"prefix":"10.1093","volume":"35","author":[{"given":"Ryosuke","family":"Nabeoka","sequence":"first","affiliation":[{"name":"Chemicals Evaluation and Research Institute Kurume Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masanori","family":"Taruki","sequence":"additional","affiliation":[{"name":"Chemicals Evaluation and Research Institute Kurume Fukuoka 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