{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T21:46:07Z","timestamp":1790286367533,"version":"4.1.0"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2015,11,25]],"date-time":"2015-11-25T00:00:00Z","timestamp":1448409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2016,4,1]],"date-time":"2016-04-01T00:00:00Z","timestamp":1459468800000},"content-version":"vor","delay-in-days":128,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Chemicals Management Plan"},{"name":"Strategic Technology Applications of Genomics for the Environment"},{"name":"Ecotoxicology and Wildlife Health Division"},{"name":"Chemicals Management Plan (CMP)"},{"name":"Strategic Technology Applications of Genomics for the Environment (STAGE)"},{"name":"Ecotoxicology and Wildlife Health Division"}],"content-domain":{"domain":["setac.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2015,11,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>\u2003<\/jats:title>\n                  <jats:p>Amid concerns about the toxicological effects and environmental prevalence of bisphenol A (BPA), efforts to find suitable, safer replacement alternatives are essential. Bisphenol S (BPS) is a potential chemical substitute for BPA; however, few studies are available confirming that it has a more desirable ecotoxicological profile. In the present study, BPS was injected into the air cell of unincubated, fertilized chicken embryos at 6 concentrations ranging from 0\u2009\u03bcg\/g to 207\u2009\u03bcg\/g egg to determine effects on pipping success, development, hepatic messenger ribonucleic acid (mRNA) expression, thyroid hormone levels, and circulating bile acid concentrations. Concentrations of BPS increased in a dose-dependent manner in whole-embryo homogenates, and exposure to the highest dose, 207\u2009\u03bcg\/g, resulted in decreased pipping success (estimated median lethal dose\u2009=\u2009279\u2009\u03bcg\/g; 95% confidence interval\u2009=\u2009161\u2013486\u2009\u03bcg\/g). Exposure to BPS also reduced growth metrics including embryo mass and tarsus length, whereas the most pronounced phenotypic effect was the concentration-dependent, significant increase in gallbladder size at concentrations \u226552.8\u2009\u03bcg\/g. These adverse phenotypic outcomes were associated with the modulation of gene targets from a chicken ToxChip polymerase chain reaction array, which are involved with xenobiotic metabolism, lipid homeostasis, bile acid synthesis, and the thyroid hormone pathway. Expression levels of 2 estrogen-responsive genes, apolipoprotein II and vitellogenin, were too low at the sampling time point assessed (i.e., pipping embryos) to quantify changes, and no effects were observed on circulating free thyroxine or bile acid concentrations. The present study provides novel, whole-animal toxicological data for a BPA replacement alternative that is not well characterized. Environ Toxicol Chem 2016;35:1541\u20131549. \u00a9 2015 SETAC<\/jats:p>\n               <\/jats:sec>","DOI":"10.1002\/etc.3313","type":"journal-article","created":{"date-parts":[[2015,11,25]],"date-time":"2015-11-25T18:44:33Z","timestamp":1448477073000},"page":"1541-1549","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Bisphenol S alters embryonic viability, development, gallbladder size, and messenger RNA expression in chicken embryos exposed via egg injection"],"prefix":"10.1093","volume":"35","author":[{"given":"Doug","family":"Crump","sequence":"first","affiliation":[{"name":"National Wildlife Research CentreEnvironment and Climate Change Canada Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne","family":"Chiu","sequence":"additional","affiliation":[{"name":"National Wildlife Research CentreEnvironment and Climate Change Canada Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim L.","family":"Williams","sequence":"additional","affiliation":[{"name":"National Wildlife Research CentreEnvironment and Climate Change Canada Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2015,11,25]]},"reference":[{"key":"2025010315272936900_etc3313-bib-0001","doi-asserted-by":"crossref","first-page":"9244","DOI":"10.1021\/es1015789","article-title":"Chemical alternatives assessment: Enabling substitution to safer chemicals","volume":"44","author":"Lavoie","year":"2010","journal-title":"Environ Sci Technol"},{"key":"2025010315272936900_etc3313-bib-0002","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1210\/er.2008-0021","article-title":"Bisphenol-A and the great divide: A review of controversies in the field of endocrine disruption","volume":"30","author":"Vandenberg","year":"2009","journal-title":"Endocr 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