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Two experiments were conducted exposing the fish to waterborne concentrations of EE2 ranging from 20\u2009ng\/L to 200\u2009ng\/L for 8\u2009wk, 12\u2009wk, and 16\u2009wk. Intersex gonads were observed after 8\u2009wk and 16\u2009wk in fish exposed to 200\u2009ng EE2\/L and 100\u2009ng EE2\/L, respectively. Oocytes\u2019 development from testis germ cells and replacement of the efferent duct periodic acid-Schiff\u2013positive secretion surrounding spermatozeugmata by parenchymal tissue and duct structure alterations were the major observed changes in the gonads. In contrast, no response was observed in the gonopodium morphology. Liver histology was also altered, showing increasing steatosis, single-cell necrosis to generalized necrosis, and disruption of acinar organization from 100\u2009ng EE2\/L to 200\u2009ng EE2\/L. In summary, the present results showed that although EE2 was not able to alter the morphology of a developed gonopodium, it was capable of inducing development of testicular oocytes in adult male C. decemmaculatus at environmentally relevant concentrations. Thus, externally normal but intersex C. decemmaculatus males would be expected in the wastewater-receiving streams that the species inhabits. According to the literature, the present study would be the first indicating estrogen-induced intersex in adult male poeciliid. Environ Toxicol Chem 2017;36:1738\u20131745. \u00a9 2016 SETAC<\/jats:p>\n               <\/jats:sec>","DOI":"10.1002\/etc.3547","type":"journal-article","created":{"date-parts":[[2016,7,6]],"date-time":"2016-07-06T11:49:05Z","timestamp":1467805745000},"page":"1738-1745","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Intersex and liver alterations induced by long-term sublethal exposure to 17\u03b1-ethinylestradiol in adult male <i>Cnesterodon decemmaculatus<\/i> (Pisces: Poeciliidae)"],"prefix":"10.1093","volume":"36","author":[{"given":"Brian Jonathan","family":"Young","sequence":"first","affiliation":[{"name":"Laboratorio de Transformaci\u00f3n de ResiduosInstituto de Microbiolog\u00eda y Zoolog\u00eda Agr\u00edcola, Instituto Nacional de Tecnolog\u00eda Agropecuaria Castelar, Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela Carina","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"Laboratorio de Ictiofisiolog\u00eda y AcuiculturaInstituto de Investigaciones Biotecnol\u00f3gicas\u2013Instituto Tecnol\u00f3gico de Chascom\u00fas (CONICET-UNSAM) Chascom\u00fas, Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego Sebasti\u00e1n","family":"Cristos","sequence":"additional","affiliation":[{"name":"Laboratorio de Contaminantes Qu\u00edmicos, Instituto de Tecnolog\u00eda de AlimentosInstituto Nacional de Tecnolog\u00eda Agropecuaria Castelar, Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana Cristina","family":"Crespo","sequence":"additional","affiliation":[{"name":"Laboratorio de Transformaci\u00f3n de ResiduosInstituto de Microbiolog\u00eda y Zoolog\u00eda Agr\u00edcola, Instituto Nacional de Tecnolog\u00eda Agropecuaria Castelar, Buenos Aires 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