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The results of the present study account for the observed differences between laboratory and field observations for Fe toxicity to algae (Pseudokirchneriella subcapitata). Results from standard 72-h assays with Fe at pH 6.3 and pH 8 resulted in similar toxicity values measured as algal biomass, with 50% effect concentrations (EC50) of 3.28\u2009mg\/L and 4.95\u2009mg\/L total Fe(III), respectively. At the end of the 72-h exposure, however, dissolved Fe concentrations were lower than 30\u2009\u03bcg\/L for all test concentrations, making a direct toxic effect of dissolved iron on algae unlikely. Analysis of nutrient concentrations in the artificial test media detected phosphorus depletion in a dose-dependent manner that correlated well with algal toxicity. Subsequent experiments adding excess phosphorus after Fe precipitation eliminated the toxicity. These results strongly suggest that observed Fe(III) toxicity on algae in laboratory conditions is a secondary effect of phosphorous depletion. Environ Toxicol Chem 2017;36:952\u2013958. \u00a9 2016 SETAC<\/jats:p>\n               <\/jats:sec>","DOI":"10.1002\/etc.3609","type":"journal-article","created":{"date-parts":[[2016,9,3]],"date-time":"2016-09-03T21:03:10Z","timestamp":1472936590000},"page":"952-958","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Effect of Fe (III) on <i>Pseudokirchneriella subcapitata<\/i> at circumneutral pH in standard laboratory tests is explained by nutrient sequestration"],"prefix":"10.1093","volume":"36","author":[{"given":"Jose J.","family":"Arbildua","sequence":"first","affiliation":[{"name":"CIMM Santiago Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"German","family":"Villavicencio","sequence":"additional","affiliation":[{"name":"CIMM Santiago Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Urrestarazu","sequence":"additional","affiliation":[{"name":"CIMM 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