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We sampled 2 contrasting sites: site 1 (upstream), surrounded by agricultural lands, and site 2 (downstream), surrounded by natural grasslands. Most of the applied pesticides (glyphosate, 2,4-D, atrazine, tebuconazole, and imidacloprid) were detected at high frequencies in surface water samples at both sites. However, only glyphosate and aminomethylphosphonic acid (AMPA) were present at high concentrations and had a significant spatial\u2013temporal trend. The highest concentrations were found during spring 2014 at site 1, in association with the intense rains that occurred in that season. The fact that glyphosate and AMPA concentrations were higher than the rest of the studied compounds is closely related to the land use within the watershed, as glyphosate was the most applied herbicide during the fallow period of glyphosate-resistant crops (soybean, maize). The pesticide mixture had a significant spatial\u2013temporal trend, reaching the highest levels during storm flow events in spring 2014. The intensive rains in spring 2014 could be the main factor influencing stream hydrology and pesticide behavior at El Crespo watershed. The estimated annual pesticide losses were 3.11\u2009g\/ha at site 1 and 0.72\u2009g\/ha at site 2. This result indicates that an attenuation process could be decreasing pesticide loads during downstream transport from site 1 to site 2. Environ Toxicol Chem 2017;36:3206\u20133216. \u00a9 2017 SETAC<\/jats:p>\n               <\/jats:sec>","DOI":"10.1002\/etc.3897","type":"journal-article","created":{"date-parts":[[2017,6,20]],"date-time":"2017-06-20T11:54:34Z","timestamp":1497959674000},"page":"3206-3216","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":104,"title":["Spatial and temporal trends and flow dynamics of glyphosate and other pesticides within an agricultural watershed in Argentina"],"prefix":"10.1093","volume":"36","author":[{"given":"D\u00e9bora J.","family":"P\u00e9rez","sequence":"first","affiliation":[{"name":"National Institute of Agricultural Technology (INTA) Balcarce Buenos Aires Argentina"},{"name":"National Scientific and Technical Research Council (CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Okada","sequence":"additional","affiliation":[{"name":"National Institute of Agricultural Technology (INTA) Balcarce Buenos Aires Argentina"},{"name":"National Scientific and Technical Research Council (CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"De Ger\u00f3nimo","sequence":"additional","affiliation":[{"name":"National Institute of Agricultural Technology (INTA) Balcarce Buenos Aires Argentina"},{"name":"National Scientific and Technical Research Council (CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirta L.","family":"Menone","sequence":"additional","affiliation":[{"name":"Institute of Marine and Coastal Research\u2013CONICET\/National University of Mar del Plata Mar del Plata Buenos Aires Argentina"},{"name":"National Scientific and Technical Research Council (CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia 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