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Due to their high water solubility, neonicotinoids can be transported to surface waters and have the potential to be toxic to aquatic life. The present study assessed and compared the acute (48- or 96-h) toxicity of 6 neonicotinoids (acetamiprid, clothianidin, dinotefuran, imidacloprid, thiacloprid, and thiamethoxam) to 21 laboratory-cultured and field-collected aquatic invertebrates spanning 10 aquatic arthropod orders. Test conditions mimicked species\u2019 habitat, with lentic taxa exposed under static conditions, and lotic taxa exposed under recirculating systems. Median lethal concentrations (LC50s) and median effect concentrations (EC50s; immobility) were calculated and used to construct separate lethal- and immobilization-derived species sensitivity distributions for each neonicotinoid, from which 5th percentile hazard concentrations (HC5s) were calculated. The results showed that the most sensitive invertebrates were insects from the orders Ephemeroptera (Neocloeon triangulifer) and Diptera (Chironomus dilutus), whereas cladocerans (Daphnia magna, Ceriodaphnia dubia) were the least sensitive. The HC5s were compared with neonicotinoid environmental concentrations from Ontario (Canada) monitoring studies. For all neonicotinoids except imidacloprid, the resulting hazard quotients indicated little to no hazard in terms of acute toxicity to aquatic communities in Ontario freshwater streams. For the neonicotinoid imidacloprid, a moderate hazard was found when only invertebrate immobilization, and not lethality, data were considered. Environ Toxicol Chem 2018;37:1430\u20131445. \u00a9 2018 SETAC<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/etc.4088","type":"journal-article","created":{"date-parts":[[2018,1,16]],"date-time":"2018-01-16T07:34:35Z","timestamp":1516088075000},"page":"1430-1445","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":167,"title":["Acute toxicity of 6 neonicotinoid insecticides to freshwater invertebrates"],"prefix":"10.1093","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8380-1548","authenticated-orcid":false,"given":"Melanie","family":"Raby","sequence":"first","affiliation":[{"name":"School of Environmental SciencesUniversity of Guelph Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Nowierski","sequence":"additional","affiliation":[{"name":"Standards Development BranchOntario Ministry of the Environment and Climate Change Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitri","family":"Perlov","sequence":"additional","affiliation":[{"name":"Department of BiologyYork University Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoming","family":"Zhao","sequence":"additional","affiliation":[{"name":"Laboratory Services BranchOntario Ministry of the Environment and Climate Change Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyan","family":"Hao","sequence":"additional","affiliation":[{"name":"Laboratory Services BranchOntario Ministry of the Environment and Climate Change Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David G.","family":"Poirier","sequence":"additional","affiliation":[{"name":"Laboratory Services BranchOntario Ministry of the Environment and Climate Change Toronto Ontario 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