{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T17:27:22Z","timestamp":1774978042094,"version":"3.50.1"},"reference-count":21,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2020,2,18]],"date-time":"2020-02-18T00:00:00Z","timestamp":1581984000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2020,3,22]],"date-time":"2020-03-22T00:00:00Z","timestamp":1584835200000},"content-version":"vor","delay-in-days":33,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["360557-2011"],"award-info":[{"award-number":["360557-2011"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001804","name":"Canada Research Chairs","doi-asserted-by":"publisher","award":["950-221924"],"award-info":[{"award-number":["950-221924"]}],"id":[{"id":"10.13039\/501100001804","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["360557\u20102011"],"award-info":[{"award-number":["360557\u20102011"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001804","name":"Canada Research Chairs","doi-asserted-by":"publisher","award":["950\u2010221924"],"award-info":[{"award-number":["950\u2010221924"]}],"id":[{"id":"10.13039\/501100001804","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["setac.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,2,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Estrogen toxicity has been an area of priority in aquatic toxicology over the last 20\u2009yr. Currently, estrogen toxicity is primarily linked to classical estrogen signaling, the interaction of estrogen receptors alpha and beta (ER\u03b1 and ER\u03b2). Recent evidence has indicated that a rapid, nongenomic, nonclassical estrogen signaling pathway exists via the G protein\u2013coupled estrogen receptor (GPER), which is expressed in many biological systems, with roles in the cardiovascular system. The objective of the present study was to investigate the effect of 17\u03b1-ethinylestradiol (EE2) on the heart rate of embryonic Japanese medaka (Oryzias latipes). A significant decrease (bradycardia) in embryonic heart rate was observed at all treatment concentrations (0.1, 1, 10, 100, and 1000\u2009ng\/L EE2) at 144, 168, and 192\u2009h postfertilization (hpf; p\u2009\u2264\u20090.05), whereas 120 and 216\u2009hpf embryos experienced a significant decrease from the control at 10, 100, and 1000\u2009ng\/L EE2 and 0.1, 100, and 1000\u2009ng\/L EE2, respectively (p\u2009\u2264\u20090.05). In addition, using select estrogen receptor modulators, it was demonstrated that estrogen-induced bradycardia appears to be linked to GPER and not ER\u03b1 and ER\u03b2. The present study highlights GPER as a novel and alternative mode of action for EE2 toxicity at environmentally relevant concentrations. Environ Toxicol Chem 2020;39:904\u2013912. \u00a9 2020 SETAC<\/jats:p>","DOI":"10.1002\/etc.4691","type":"journal-article","created":{"date-parts":[[2020,2,19]],"date-time":"2020-02-19T03:20:01Z","timestamp":1582082401000},"page":"904-912","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The Effects of 17\u03b1-Ethinylestradiol on the Heart Rate of Embryonic Japanese Medaka (<i>Oryzias latipes<\/i>)"],"prefix":"10.1093","volume":"39","author":[{"given":"Jordan Christopher","family":"Anderson","sequence":"first","affiliation":[{"name":"Faculty of Science, Ontario Tech University, Oshawa Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lindsay","family":"Beyger","sequence":"additional","affiliation":[{"name":"Faculty of Science, Ontario Tech University, Oshawa Ontario 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