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Although frozen tissues are preferable for trace element analysis, formalin-fixed tissues are often the only samples available; however, it is uncertain whether formalin fixation alters tissue trace element concentrations. To explore whether formalin-fixed tissues could be utilized for toxicology studies, concentrations of 14 trace elements (arsenic [As], cadmium, cobalt, chromium, copper, iron, mercury, manganese, nickel, lead, selenium, tin, vanadium, and zinc [Zn]) were measured in frozen and formalin-fixed bottlenose dolphin (Tursiops truncatus) tissues following short-term (6\u2009wk; tissues: blubber, liver, and lung) and long-term preservation (3\u20137\u2009yr; tissues: blubber, brain, kidney, liver, lung, and skin) using inductively coupled plasma mass spectrometry. Following both short-term and long-term preservation, there were significant differences in tissue trace element concentrations between preservation methods. Some trace elements were found in greater concentrations in frozen tissues compared with formalin-fixed tissues, suggesting leaching (e.g., mean As concentrations were between 1.4 and 7.6 times greater in frozen tissues). In contrast, other trace elements were found in greater concentrations in formalin-fixed tissues compared with frozen tissues, suggesting contamination (e.g., mean Zn concentrations were up to 8.7 times higher in some formalin-fixed tissues). Our results suggest that it may be possible to account for the effects of formalin fixation for some trace elements, but leaching and contamination should be carefully considered. 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