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Because oil toxicity can be modulated by weathering of the petroleum products, the present study aimed to assess changes in fish embryotoxicity to mixed sweet blend crude oil as it weathered at air and water temperatures of 14\u2009\u00b0C and 15\u2009\u00b0C, respectively, for 28\u2009d. The physicochemical behavior of the oil was also monitored. Water samples were taken from the spill tank 5 times during the 28-d experiment on days 1, 6, 14, 21, and 28 and were used to perform toxicity exposures using fathead minnow embryos (Pimephales promelas). For each water sampling day, newly fertilized embryos were exposed to a serial dilution of the spill tank water, noncontaminated river water (used in the spill tank), and a reconstituted water laboratory control. Embryos were raised until hatching. Although mortality was not significantly altered by the oil contamination over the time period, malformation occurrence and severity showed concentration-dependent responses to all contaminated water collected. The results suggest that days 14, 21, and 28 were the most toxic time periods for the fish embryos, which corresponded to increasing concentrations of unidentified oxidized organic compounds detected by a quadropole\u2013time-of-flight system. The present study highlights a novel area for oil research, which could help us to better understand the toxicity associated with oil weathering for aquatic species. Environ Toxicol Chem 2021;40:127\u2013138. \u00a9 2020 Her Majesty the Queen in Right of Canada. Reproduced with the permission of the Minister of Natural Resources Canada.<\/jats:p>","DOI":"10.1002\/etc.4891","type":"journal-article","created":{"date-parts":[[2020,10,5]],"date-time":"2020-10-05T15:33:27Z","timestamp":1601912007000},"page":"127-138","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Fate and Fathead Minnow Embryotoxicity of Weathering Crude Oil in a Pilot-Scale Spill Tank"],"prefix":"10.1093","volume":"40","author":[{"given":"Lara R.","family":"Lara-Jacobo","sequence":"first","affiliation":[{"name":"Centre Eau Terre Environnement Institut national de la recherche scientifique, Qu\u00e9bec City Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Gauthier","sequence":"additional","affiliation":[{"name":"Centre Eau Terre Environnement Institut national de la recherche scientifique, Qu\u00e9bec City Qu\u00e9bec 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