{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T01:36:03Z","timestamp":1782264963876,"version":"3.54.5"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2023,7,11]],"date-time":"2023-07-11T00:00:00Z","timestamp":1689033600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T00:00:00Z","timestamp":1692230400000},"content-version":"vor","delay-in-days":37,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP22H03760"],"award-info":[{"award-number":["JP22H03760"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Expenditure to Support Issue-based Interdisciplinary Research Education for the School of Interdisciplinary Science and Innovation at Kyushu University"},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP22H03760"],"award-info":[{"award-number":["JP22H03760"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["setac.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The potential of pharmaceuticals and personal care products to alter the behavior of aquatic organisms is a growing concern. To assess the actual effect of these substances on aquatic organisms, a simple but effective behavioral test is required. We devised a simple behavioral (Peek-A-Boo) test to assess the effect of anxiolytics on the behavior of a model fish (medaka, Oryzias latipes). In the Peek-A-Boo test, we investigated the response of medaka to an image of a predator fish (donko fish, Odontobutis obscura). The test revealed that the time taken for test medaka exposed to diazepam (0.8, 4, 20, or 100\u2009\u00b5g\/L) to approach the image was shorter by a factor of 0.22 to 0.65, and the time spent in the area close to the image was longer by a factor of 1.8 to 2.7 than in the solvent control group for all diazepam exposure groups (p\u2009&amp;lt;\u20090.05). Hence, we confirmed that the test could detect changes in medaka behavior caused by diazepam with high sensitivity. The Peek-A-Boo test we devised is a simple behavioral test with high sensitivity for fish behavioral alteration. Environ Toxicol Chem 2023;42:2358\u20132363. \u00a9 2023 SETAC.<\/jats:p>","DOI":"10.1002\/etc.5713","type":"journal-article","created":{"date-parts":[[2023,7,11]],"date-time":"2023-07-11T11:53:55Z","timestamp":1689076435000},"page":"2358-2363","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Peek-A-Boo Test: A Simple Test for Assessing the Effect of Anxiolytics on Fish Behavior"],"prefix":"10.1093","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6506-5313","authenticated-orcid":false,"given":"Yuki","family":"Takai","sequence":"first","affiliation":[{"name":"Laboratory of Marine Environmental Science, Faculty of Agriculture Kyushu University Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mutsumi","family":"Izumi","sequence":"additional","affiliation":[{"name":"Laboratory of Marine Environmental Science, Faculty of Agriculture Kyushu University Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuriko","family":"Motoyama","sequence":"additional","affiliation":[{"name":"Laboratory of Marine Environmental Science, Faculty of Agriculture Kyushu University Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yohei","family":"Shimasaki","sequence":"additional","affiliation":[{"name":"Laboratory of Marine Environmental Science, Faculty of Agriculture Kyushu University Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuji","family":"Oshima","sequence":"additional","affiliation":[{"name":"Institute of Nature and Environmental Technology Kanazawa University Ishikawa Japan"},{"name":"Laboratory of Marine Environmental Science, Faculty of Agriculture Kyushu University Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ik Joon","family":"Kang","sequence":"additional","affiliation":[{"name":"School of Interdisciplinary Science and Innovation Kyushu University Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,7,11]]},"reference":[{"issue":"2","key":"2025010313384938200_etc5713-bib-0001","doi-asserted-by":"crossref","first-page":"R13","DOI":"10.1530\/REP-20-0154","article-title":"Reproduction in a polluted world: Implications for wildlife","volume":"160","author":"Aulsebrook","year":"2020","journal-title":"Reproduction"},{"issue":"1","key":"2025010313384938200_etc5713-bib-0002","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.pbb.2009.07.009","article-title":"Buspirone, chlordiazepoxide and diazepam effects in a zebrafish model of anxiety","volume":"94","author":"Bencan","year":"2009","journal-title":"Pharmacology, Biochemistry and Behavior"},{"issue":"4","key":"2025010313384938200_etc5713-bib-0003","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.1111\/brv.12844","article-title":"Frontiers in quantifying wildlife behavioural responses to chemical pollution","volume":"97","author":"Bertram","year":"2022","journal-title":"Biological Reviews of the Cambridge Philosophical Society"},{"issue":"2","key":"2025010313384938200_etc5713-bib-0004","first-page":"e2196","article-title":"Determination of carbamazepine and diazepam by SPE-HPLC-DAD in Bel\u00e9m River water, Curitiba-PR\/Brazil","volume":"13","author":"B\u00f6ger","year":"2018","journal-title":"Revista Ambiente & \u00c1gua"},{"issue":"6121","key":"2025010313384938200_etc5713-bib-0005","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1126\/science.1226850","article-title":"Dilute concentrations of a psychiatric drug alter behavior of fish from natural populations","volume":"339","author":"Brodin","year":"2013","journal-title":"Science"},{"issue":"Pt 1","key":"2025010313384938200_etc5713-bib-0006","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1016\/j.scitotenv.2018.09.046","article-title":"The pharmaceutical pollutant fluoxetine alters reproductive behaviour in a fish independent of predation risk","volume":"650","author":"Fursdon","year":"2019","journal-title":"The Science of the Total Environment"},{"key":"2025010313384938200_etc5713-bib-0007","doi-asserted-by":"crossref","first-page":"28986","DOI":"10.1038\/srep28986","article-title":"Environmental and pharmacological manipulations blunt the stress response of zebrafish in a similar manner","volume":"6","author":"Giacomini","year":"2016","journal-title":"Scientific Reports"},{"issue":"24","key":"2025010313384938200_etc5713-bib-0008","doi-asserted-by":"crossref","first-page":"16299","DOI":"10.1021\/acs.est.1c04724","article-title":"The potential for adverse effects in fish exposed to antidepressants in the aquatic environment","volume":"55","author":"Gould","year":"2021","journal-title":"Environmental Science & Technology"},{"issue":"1","key":"2025010313384938200_etc5713-bib-0009","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1002\/aur.2620","article-title":"Duloxetine ameliorates valproic acid-induced hyperactivity, anxiety-like behavior, and social interaction deficits in zebrafish","volume":"15","author":"Joseph","year":"2022","journal-title":"Autism Research"},{"key":"2025010313384938200_etc5713-bib-0010","doi-asserted-by":"crossref","first-page":"1","DOI":"10.18637\/jss.v064.i09","article-title":"nparcomp: An R software package for nonparametric multiple comparisons and simultaneous confidence intervals","volume":"64","author":"Konietschke","year":"2015","journal-title":"Journal of Statistical Software"},{"issue":"8","key":"2025010313384938200_etc5713-bib-0011","doi-asserted-by":"crossref","first-page":"e0181010","DOI":"10.1371\/journal.pone.0181010","article-title":"Impact of stress, fear and anxiety on the nociceptive responses of larval zebrafish","volume":"12","author":"Lopez-Luna","year":"2017","journal-title":"PLoS One"},{"key":"2025010313384938200_etc5713-bib-0012","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1016\/j.envpol.2016.10.010","article-title":"The psychoactive pollutant fluoxetine compromises antipredator behaviour in fish","volume":"222","author":"Martin","year":"2017","journal-title":"Environmental Pollution"},{"issue":"9","key":"2025010313384938200_etc5713-bib-0013","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1016\/j.tree.2022.05.009","article-title":"Predicting the impacts of chemical pollutants on animal groups","volume":"37","author":"Michelangeli","year":"2022","journal-title":"Trends in Ecology & Evolution"},{"key":"2025010313384938200_etc5713-bib-0014","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.jes.2019.02.025","article-title":"Degradation of duloxetine: Identification of transformation products by UHPLC-ESI(+)-HRMS\/MS, in silico toxicity and wastewater analysis","volume":"82","author":"Osawa","year":"2019","journal-title":"Journal of Environmental Sciences"},{"key":"2025010313384938200_etc5713-bib-0015","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.aquatox.2015.12.019","article-title":"Low levels of chemical anthropogenic pollution may threaten amphibians by impairing predator recognition","volume":"172","author":"Polo-Cavia","year":"2016","journal-title":"Aquatic Toxicology"},{"key":"2025010313384938200_etc5713-bib-0016","author":"Python","year":"2023"},{"key":"2025010313384938200_etc5713-bib-0017","author":"R Core Team","year":"2022"},{"issue":"6","key":"2025010313384938200_etc5713-bib-0018","doi-asserted-by":"crossref","first-page":"e03527","DOI":"10.1002\/ecs2.3527","article-title":"Exposure to a common antidepressant alters crayfish behavior and has potential subsequent ecosystem impacts","volume":"12","author":"Reisinger","year":"2021","journal-title":"Ecosphere"},{"issue":"1","key":"2025010313384938200_etc5713-bib-0019","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.bbr.2011.11.041","article-title":"On the edge: Pharmacological evidence for anxiety-related behavior in zebrafish larvae","volume":"228","author":"Richendrfer","year":"2012","journal-title":"Behavioural Brain Research"},{"issue":"1885","key":"2025010313384938200_etc5713-bib-0020","doi-asserted-by":"crossref","first-page":"20181297","DOI":"10.1098\/rspb.2018.1297","article-title":"Direct and indirect effects of chemical contaminants on the behaviour, ecology and evolution of wildlife","volume":"285","author":"Saaristo","year":"2018","journal-title":"Proceedings of the Royal Society B"},{"key":"2025010313384938200_etc5713-bib-0021","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.aquatox.2016.12.007","article-title":"Impacts of the antidepressant fluoxetine on the anti-predator behaviours of wild guppies (Poecilia reticulata)","volume":"183","author":"Saaristo","year":"2017","journal-title":"Aquatic Toxicology"},{"key":"2025010313384938200_etc5713-bib-0022","doi-asserted-by":"crossref","first-page":"161324","DOI":"10.1016\/j.scitotenv.2022.161324","article-title":"Effects of common antiepileptic drugs on teleost fishes","volume":"866","author":"Salahinejad","year":"2023","journal-title":"The Science of the Total Environment"},{"issue":"8","key":"2025010313384938200_etc5713-bib-0023","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1111\/eth.12630","article-title":"Validation of 2D-animated pictures as an investigative tool in the behavioural sciences: A case study with a West African cichlid fish, Pelvicachromis pulcher","volume":"123","author":"Scherer","year":"2017","journal-title":"Ethology: Formerly Zeitschrift Fur Tierpsychologie"},{"issue":"1\u20132","key":"2025010313384938200_etc5713-bib-0024","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.marpolbul.2017.06.067","article-title":"Anthropogenic noise compromises the anti-predator behaviour of the European seabass, Dicentrarchus labrax (L.)","volume":"122","author":"Spiga","year":"2017","journal-title":"Marine Pollution Bulletin"},{"key":"2025010313384938200_etc5713-bib-0025","doi-asserted-by":"crossref","first-page":"134403","DOI":"10.1016\/j.chemosphere.2022.134403","article-title":"Combined effect of diazepam and polystyrene microplastics on the social behavior of medaka (Oryzias latipes)","volume":"299","author":"Takai","year":"2022","journal-title":"Chemosphere"},{"issue":"3","key":"2025010313384938200_etc5713-bib-0026","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1080\/17470910802625215","article-title":"Pharmacological effects on mirror approaching behavior and neurochemical aspects of the telencephalon in the fish, medaka (Oryzias latipes)","volume":"4","author":"Tsubokawa","year":"2009","journal-title":"Social Neuroscience"},{"issue":"16","key":"2025010313384938200_etc5713-bib-0027","doi-asserted-by":"crossref","first-page":"jeb182469","DOI":"10.1242\/jeb.182469","article-title":"UMATracker: An intuitive image-based tracking platform","volume":"221","author":"Yamanaka","year":"2018","journal-title":"The Journal of Experimental Biology"}],"container-title":["Environmental Toxicology and Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/etc\/article-pdf\/42\/11\/2358\/59770864\/etc5713.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/etc\/article-pdf\/42\/11\/2358\/59770864\/etc5713.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,3]],"date-time":"2025-01-03T13:54:18Z","timestamp":1735912458000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/etc\/article\/42\/11\/2358\/7729714"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,11]]},"references-count":27,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2023,7,11]]},"published-print":{"date-parts":[[2023,11,1]]}},"URL":"https:\/\/doi.org\/10.1002\/etc.5713","relation":{},"ISSN":["0730-7268","1552-8618"],"issn-type":[{"value":"0730-7268","type":"print"},{"value":"1552-8618","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2023,11,1]]},"published":{"date-parts":[[2023,7,11]]},"assertion":[{"value":"2023-04-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-07-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}