{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T12:09:01Z","timestamp":1780488541688,"version":"3.54.1"},"reference-count":94,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2024,12,6]],"date-time":"2024-12-06T00:00:00Z","timestamp":1733443200000},"content-version":"vor","delay-in-days":219,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T00:00:00Z","timestamp":1710288000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["setac.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The amount of Sargassum spp. arriving in the Caribbean Sea has increased steadily in the last few years, producing a profound environmental impact on the ecological dynamics of the coasts of the Yucatan Peninsula. We characterized the toxicological effects of an ethanolic extract of Sargassum spp. on zebrafish (Danio rerio) embryos (ZFEs) in a 96-h static bioassay using T1 (0.01\u2009mg\/L), T2 (0.1\u2009mg\/L), T3 (1\u2009mg\/L), T4 (10\u2009mg\/L), T5 (25\u2009mg\/L), T6 (50\u2009mg\/L), T7 (75\u2009mg\/L), T8 (100\u2009mg\/L), T9 (200\u2009mg\/L), and T10 (400\u2009mg\/L). In this extract, we detected 74 compounds by gas chromatography-mass spectrometry (GC-MS), of which hexadecanoic acid methyl ester, and 2-pentanone 4-hydroxy-4-methyl, were the most abundant. In ZFEs, a median lethal concentration of 251\u2009mg\/L was estimated. Exposed embryos exhibited extensive morphological changes, including edema in the yolk sac, scoliosis, and loss of pigmentation, as well as malformations of the head, tail, and eyes. By integrating these abnormalities using the Integrated Biological Response (IBRv2) and General Morphological Score (GMS) indices, we were able to determine that ZFEs exposed to 200\u2009mg\/L (T9) exhibited the most pronounced biological response in comparison with the other groups. In the comparative transcriptomic analysis, 66 genes were upregulated, and 246 genes were downregulated in the group exposed to 200\u2009mg\/L compared with the control group. In the upregulated genes, we identified several gene ontology-enriched terms, such as response to xenobiotic stimuli, cellular response to chemical stimulus, transcriptional regulation, pigment metabolic process, erythrocyte differentiation and embryonic hemopoiesis, extracellular matrix organization, and chondrocyte differentiation involved in endochondral bone morphogenesis, among others. In the down-regulated genes, we found many genes associated with nervous system processes, sensory and visual perception, response to abiotic stimulus, and the nucleoside phosphate biosynthetic process. The probable connections among the morphological changes observed in the transcriptome are thoroughly discussed. Our findings suggest that Sargassum spp. exposure can induce a wide negative impact on zebrafish embryos. Environ Toxicol Chem 2024;43:1075\u20131089. \u00a9 2024 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.<\/jats:p>","DOI":"10.1002\/etc.5840","type":"journal-article","created":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T13:59:17Z","timestamp":1710338357000},"page":"1075-1089","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<i>Sargassum<\/i> spp. Ethanolic Extract Elicits Toxic Responses and Malformations in Zebrafish (<i>Danio rerio<\/i>) Embryos"],"prefix":"10.1093","volume":"43","author":[{"given":"Carlos E.","family":"Gonz\u00e1lez-Penagos","sequence":"first","affiliation":[{"name":"Departamento de Recursos del Mar, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional, Unidad M\u00e9rida, M\u00e9rida, Yucat\u00e1n, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas Alejandro","family":"Zamora-Brise\u00f1o","sequence":"additional","affiliation":[{"name":"Red de Estudios Moleculares Avanzados, Campus III, Instituto de Ecolog\u00eda, Xalapa, Veracruz, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f3nica","family":"Am\u00e9ndola-Pimenta","sequence":"additional","affiliation":[{"name":"Departamento de Recursos del Mar, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional, Unidad M\u00e9rida, M\u00e9rida, Yucat\u00e1n, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanis","family":"Cruz-Quintana","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Sanidad Acu\u00edcola, Inocuidad y Salud Ambiental. Departamento de Acuicultura, Pesca y Recursos Naturales Renovables. Facultad de Acuicultura y Ciencias del Mar, Universidad T\u00e9cnica de Manab\u00ed, Bah\u00eda de Car\u00e1quez, Manab\u00ed, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana M.","family":"Santana-Pi\u00f1eros","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Sanidad Acu\u00edcola, Inocuidad y Salud Ambiental. Departamento de Acuicultura, Pesca y Recursos Naturales Renovables. Facultad de Acuicultura y Ciencias del Mar, Universidad T\u00e9cnica de Manab\u00ed, Bah\u00eda de Car\u00e1quez, Manab\u00ed, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas R.","family":"Torres-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Centro Interdisciplinario de Investigaci\u00f3n para el Desarrollo Integral Regional (CIIDIR) Unidad Michoac\u00e1n M\u00e9xico"},{"name":"Consejo Nacional de Humanidades, Ciencias y Tecnolog\u00edas (CONAHCYT) Ciudad de M\u00e9xico M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayra A.","family":"Ca\u00f1izares-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Departamento de Recursos del Mar, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional, Unidad M\u00e9rida, M\u00e9rida, Yucat\u00e1n, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan A.","family":"P\u00e9rez-Vega","sequence":"additional","affiliation":[{"name":"Departamento de Recursos del Mar, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional, Unidad M\u00e9rida, M\u00e9rida, Yucat\u00e1n, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana C.","family":"Pe\u00f1uela-Mendoza","sequence":"additional","affiliation":[{"name":"Departamento de Recursos del Mar, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional, Unidad M\u00e9rida, M\u00e9rida, Yucat\u00e1n, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0469-1489","authenticated-orcid":false,"given":"Rossanna","family":"Rodr\u00edguez-Canul","sequence":"additional","affiliation":[{"name":"Departamento de Recursos del Mar, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional, Unidad M\u00e9rida, M\u00e9rida, Yucat\u00e1n, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,5,1]]},"reference":[{"issue":"8","key":"2025010612333207900_etc5840-bib-0001","doi-asserted-by":"crossref","first-page":"1610","DOI":"10.1093\/hmg\/ddr038","article-title":"A complex regulatory network of transcription factors critical for ocular development and disease","volume":"20","author":"Acharya","year":"2011","journal-title":"Human Molecular Genetics"},{"issue":"12","key":"2025010612333207900_etc5840-bib-0002","doi-asserted-by":"crossref","first-page":"6430","DOI":"10.3390\/ijms22126430","article-title":"Null cyp1b1 activity in zebrafish leads to variable craniofacial defects associated with altered expression of extracellular matrix and lipid metabolism genes","volume":"22","author":"Alexandre-Moreno","year":"2021","journal-title":"International Journal of Molecular Sciences"},{"issue":"2","key":"2025010612333207900_etc5840-bib-0003","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1002\/ece3.2630","article-title":"Comparative mitochondrial and chloroplast genomics of a genetically distinct form of Sargassum contributing to recent \u201cGolden Tides\u201d in the Western Atlantic","volume":"7","author":"Amaral-Zettler","year":"2017","journal-title":"Ecology and Evolution"},{"issue":"8","key":"2025010612333207900_etc5840-bib-0004","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1016\/j.biochi.2003.09.006","article-title":"A role for AP-1 in apoptosis: The case for and against","volume":"85","author":"Ameyar","year":"2003","journal-title":"Biochimie"},{"key":"2025010612333207900_etc5840-bib-0005","article-title":"A quality control tool for high throughput sequence data. 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