{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T05:16:56Z","timestamp":1783833416181,"version":"3.55.0"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2009,12,10]],"date-time":"2009-12-10T00:00:00Z","timestamp":1260403200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2009,12,10]],"date-time":"2009-12-10T00:00:00Z","timestamp":1260403200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["setac.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2009,12,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The sterlet (Acipenser ruthenus L.) is a bottom-feeding fish species with a direct exposure to contaminants from water and sediments. Although heavy metal pollution is believed to be one of the main threats to the sterlet population in the Danube River basin, there is a lack of knowledge of the exact impact of heavy metals on their survival. In the present study, effects of heavy metal pollution on sterlet in the Danube basin were assessed as well as the utility of different sterlet organs and tissues as indicators of heavy metal contamination. The sterlet were sampled at three different sites in the Danube basin, in Hungary and Serbia, isolated from each other by dams. Heavy metal analysis included measurement of Cd, As, Pb, Cr, Hg, Cu, Ni, Fe, Mn, and Zn concentrations in sterlet gills, muscle, liver, and intestine, and histopathological analyses comprised assessment and scoring of the extent and intensity of alterations in skin, gills, and liver tissue. Analysis revealed a significant presence of sublethal histopathological changes that were most pronounced in the liver and skin and increased accumulation of heavy metals, with the highest concentrations in the liver. Canonical discriminant analysis showed significant differentiation among the three studied localities, suggesting that the heavy metal concentrations in sterlet populations were site specific. The present study concludes that the accumulation of heavy metals is a response to the presence of these pollutants in the environment, and, together with other pollutants, it affects the vital organs of natural sterlet populations. Environ. Toxicol. Chem. 2010;29:515\u2013521. \u00a9 2009 SETAC<\/jats:p>","DOI":"10.1002\/etc.82","type":"journal-article","created":{"date-parts":[[2010,3,17]],"date-time":"2010-03-17T00:00:11Z","timestamp":1268784011000},"page":"515-521","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":100,"title":["Liver, gills, and skin histopathology and heavy metal content of the Danube sterlet (<i>Acipenser ruthenus<\/i> Linnaeus, 1758)"],"prefix":"10.1093","volume":"29","author":[{"given":"Vesna","family":"Poleksic","sequence":"first","affiliation":[{"name":"Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11081 Zemun, Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirjana","family":"Lenhardt","sequence":"additional","affiliation":[{"name":"Institute for Biological Research, Despota Stefana 142, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Jaric","sequence":"additional","affiliation":[{"name":"Institute for Multidisciplinary Research, Kneza Viseslava 1, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dragana","family":"Djordjevic","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Njegoseva 12, 11001 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoran","family":"Gacic","sequence":"additional","affiliation":[{"name":"Institute for Multidisciplinary Research, Kneza Viseslava 1, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gorcin","family":"Cvijanovic","sequence":"additional","affiliation":[{"name":"Institute for Multidisciplinary Research, Kneza Viseslava 1, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bozidar","family":"Raskovic","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11081 Zemun, Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2009,12,10]]},"reference":[{"key":"2025010316450853400_bib1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1046\/j.1365-2761.1999.00134.x","article-title":"Histopathology in fish: Proposal for a protocol to assess aquatic pollution","volume":"22","author":"Bernet","year":"1999","journal-title":"J Fish Dis"},{"key":"2025010316450853400_bib2","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S1382-6689(02)00126-6","article-title":"Fish bioaccumulation and biomarkers in environmental risk assessment: A review","volume":"13","author":"Van der Oost","year":"2003","journal-title":"Environ Toxicol Pharmacol"},{"key":"2025010316450853400_bib3","first-page":"153","author":"Lenhardt","year":"2004"},{"key":"2025010316450853400_bib4","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1023\/A:1007381418332","article-title":"Present status of commercial stocks of sturgeons in the Caspian Sea basin","volume":"48","author":"Khodorevskaya","year":"1997","journal-title":"Environ Biol Fish"},{"key":"2025010316450853400_bib5","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1002\/1522-2632(200211)87:5\/6<483::AID-IROH483>3.0.CO;2-K","article-title":"Status and management of Eurasian sturgeon: An overview","volume":"87","author":"Williot","year":"2002","journal-title":"Int Rev Hydrobiol"},{"key":"2025010316450853400_bib6","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1016\/j.marpolbul.2004.06.008","article-title":"Concentrations of trace elements in muscle of sturgeons in the Caspian Sea","volume":"49","author":"Agusa","year":"2004","journal-title":"Mar Pollut Bull"},{"key":"2025010316450853400_bib7","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1111\/j.1467-2979.2005.00190.x","article-title":"Status, trends and management of sturgeon and paddlefish fisheries","volume":"6","author":"Pikitch","year":"2005","journal-title":"Fish Fish"},{"issue":"Suppl 1","key":"2025010316450853400_bib8","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/j.1439-0426.2007.00924.x","article-title":"Sturgeons in the Slovakian rivers of the Danube River basin: an overview of their current status and proposal for their conservation and restoration","volume":"22","author":"Hol\u010dik","year":"2006","journal-title":"J Appl Ichthyol"},{"key":"2025010316450853400_bib9","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1023\/A:1007343611333","article-title":"Endangered migratory sturgeons of the lower Danube River and its delta","volume":"48","author":"Bacalbasa-Dobrovici","year":"1997","journal-title":"Environ Biol Fish"},{"key":"2025010316450853400_bib10","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/BF01875645","article-title":"Hepatocellular adaptation to extreme nutritional conditions in ide, Leuciscus idus melanotus L. 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