{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T16:52:17Z","timestamp":1786121537698,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T00:00:00Z","timestamp":1774828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T00:00:00Z","timestamp":1774828800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19H03129"],"award-info":[{"award-number":["19H03129"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23H02387"],"award-info":[{"award-number":["23H02387"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Culture protocols need to yield 100 million equine synovial fluid (SF)\u2010derived mesenchymal stromal cells (SF\u2010MSCs) in around 3\u2009weeks are needed, before these cells can be evaluated as agents of articular repair in clinical trials.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To investigate mass culture of equine SF\u2010MSC culture protocols using nonwoven polyethylene terephthalate (PET) fabrics for the potential to meet the targets for clinical trials.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design<\/jats:title>\n                    <jats:p>In vitro experiments.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      SF samples were collected from the carpal joints in thoroughbred racehorses (\n                      <jats:italic>n<\/jats:italic>\n                      =\u200921) undergoing arthroscopic surgery and cultured in 10% fetal bovine serum (FBS\u2010protocol;\n                      <jats:italic>n<\/jats:italic>\n                      =\u20097) or 10% equine serum (ES\u2010protocol;\n                      <jats:italic>n<\/jats:italic>\n                      =\u20097)\u2010supplemented medium. Primary (P0) SF\u2010MSCs were isolated by dish culture and then passaged on nonwoven PET fabrics in culture bottles at a seeding density of 1 \u00d7\u200910\n                      <jats:sup>4<\/jats:sup>\n                      cells\/strip. The other 7 SF samples were cultured in ES\u2010supplemented medium, and P0\u2010SF\u2010MSCs were passaged at a higher density using an automated culture device (ACD\u2010protocol;\n                      <jats:italic>n<\/jats:italic>\n                      =\u20097).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Under the FBS and ES protocols, 0.84 \u00b1\u20090.82 \u00d7\u200910\n                      <jats:sup>7<\/jats:sup>\n                      and 1.40 \u00b1\u20090.79 \u00d7\u200910\n                      <jats:sup>7<\/jats:sup>\n                      P0\u2010SF\u2010MSCs were isolated, and 1.34 \u00b1\u20090.66 \u00d7\u200910\n                      <jats:sup>8<\/jats:sup>\n                      and 0.94 \u00b1\u20090.20 \u00d7\u200910\n                      <jats:sup>8<\/jats:sup>\n                      passage 1 (P1)\u2010SF\u2010MSCs were harvested. The respective total durations of primary and passage culture were 21.4 \u00b1\u20091.8 and 27.6 \u00b1\u20092.1\u2009days duration. An ACD\u2010protocol yielded 0.71 \u00b1\u20090.32 \u00d7\u200910\n                      <jats:sup>7<\/jats:sup>\n                      P0\u2010SF\u2010MSCs, from which 0.85 \u00b1\u20090.48 \u00d7\u200910\n                      <jats:sup>8<\/jats:sup>\n                      of P1\u2010SF\u2010MSCs were cultured in 20.6 \u00b1\u20091.1\u2009days.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Main Limitations<\/jats:title>\n                    <jats:p>Visualisation of SF\u2010MSC attached to PET nonwoven fabric, counting of SF\u2010MSCs attached to and detached from PET nonwoven fabric, and daily quantification of SF\u2010MSC proliferating on fabrics.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>To support progress to clinical trials, of a sufficient number (100 million) of equine SF\u2010MSCs need to be harvested in around 3\u2009weeks. These goals were achieved with the FBS protocol, but further optimisation is needed for the ES protocol. Our findings on in vitro MSCs amplification suggest that SF, a potential source for non\u2010invasively collected MSCs, is available as a promising matrix for therapeutic strategies using autologous transplantation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/evj.70152","type":"journal-article","created":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T03:19:53Z","timestamp":1774927193000},"page":"1454-1466","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mass culture of equine synovial fluid\u2010derived mesenchymal stromal cells using nonwoven polyethylene terephthalate fabrics"],"prefix":"10.1002","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-1099-7040","authenticated-orcid":false,"given":"Miho Daniel","family":"Yoshitomi","sequence":"first","affiliation":[{"name":"Joint Graduate School of Veterinary Medicine Kagoshima University  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohide","family":"Kuramoto","sequence":"additional","affiliation":[{"name":"Joint Graduate School of Veterinary Medicine Kagoshima University  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Hatazoe","sequence":"additional","affiliation":[{"name":"Joint Graduate School of Veterinary Medicine Kagoshima University  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenta","family":"Mitsuda","sequence":"additional","affiliation":[{"name":"Rittoh Training Center Japan Racing Association  Rittoh Shiga Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry","family":"Smith","sequence":"additional","affiliation":[{"name":"Joint Graduate School of Veterinary Medicine Kagoshima University  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Misumi","sequence":"additional","affiliation":[{"name":"Joint Graduate School of Veterinary Medicine Kagoshima University  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,30]]},"reference":[{"key":"e_1_2_12_2_1","first-page":"87","volume-title":"Joint disease in the horse","author":"Mcllwraith CW","year":"1996"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.71.11.1284"},{"key":"e_1_2_12_4_1","first-page":"40","volume-title":"Joint disease in the horse","author":"Mcllwraith CW","year":"1996"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-200101000-00008"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.1982.180.03.239"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2015.10.027"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2006.11.009"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2019.02.036"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2018.0061"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22052499"},{"key":"e_1_2_12_12_1","first-page":"506","volume-title":"Proceedings of the American Association of Equine Practitioners","author":"Frisbie DD","year":"2014"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2014.07.029"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/7416493"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani11071918"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-021-03126-y"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1634"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/1947603511406531"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2011.04.067"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.TEC.2018.021922"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2006.01.010"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.14712\/fb2009055010027"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.21212"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.21651"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar3869"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/1529-4190131(200108)44:8<1928:AID-ART331>3.0.CO;2-P"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v022a21"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.3109\/14653249.2011.638912"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2019.04.002"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2017.215"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-018-0876-3"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2121-7-14"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.2012.12488"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.22029"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcyt.2013.12.003"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.12.068"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.4161\/biom.22170"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2025.105897"}],"container-title":["Equine Veterinary Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/beva.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/evj.70152","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/beva.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/evj.70152","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/beva.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/evj.70152","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T05:12:03Z","timestamp":1786079523000},"score":1,"resource":{"primary":{"URL":"https:\/\/beva.onlinelibrary.wiley.com\/doi\/10.1002\/evj.70152"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,30]]},"references-count":37,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1002\/evj.70152"],"URL":"https:\/\/doi.org\/10.1002\/evj.70152","archive":["Portico"],"relation":{},"ISSN":["0425-1644","2042-3306"],"issn-type":[{"value":"0425-1644","type":"print"},{"value":"2042-3306","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,30]]},"assertion":[{"value":"2025-10-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}