{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:16:46Z","timestamp":1784593006850,"version":"3.55.0"},"reference-count":23,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100014013","name":"UK Research and Innovation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014013","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n Equine foals receive IgG from mare colostrum through passive transfer. Failure of passive transfer (FPT) is a significant risk to the foal's life, leaving them vulnerable to infection and sepsis. Radial Immunodiffusion (RID) and immunoturbidimetric assays quantify IgG present in a foal sample but require a laboratory to complete. Accurate, reproducible, stall\u2010side testing to rapidly quantify IgG would allow for expedited clinical decisions, with potential to improve equine foal care and survival.<\/jats:p>\n <\/jats:sec>\n \n Objectives<\/jats:title>\n To evaluate the analytical and clinical performance of a stall\u2010side IgG lateral\u2010flow test and reader (Sidekick), assessing its use as a point\u2010of\u2010care (POC) test to provide reliable results and agreement of IgG quantification with gold\u2010standard methods.<\/jats:p>\n <\/jats:sec>\n \n Study Design<\/jats:title>\n Retrospective cohort.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n Stall\u2010side LFD IgG test (Sidekick) was assessed with serial diluted foal sample (0.8\u201320\u2009g\/L, three replicates) and Stall\u2010side LFD was compared to RID and Immunoturbidimetric assay. Serum\/plasma samples from 10 foals created two cohorts, one representing successful passive transfer and one representing failed passive transfer. Agreement in IgG quantification between matched blood and plasma samples by the stall\u2010side LFD was tested in three foals.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n The Stall\u2010side LFD IgG test showed excellent recovery and high repeatability, 96.3%\u2013109.5% across the clinically relevant range of 4\u20138\u2009g\/L (CV% 3.7\u201310). Overall, there was strong agreement with the reference method and strong agreement between matched whole\u2010blood and plasma samples.<\/jats:p>\n <\/jats:sec>\n \n Main Limitations<\/jats:title>\n Small cohort (10 foals).<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n The stall\u2010side IgG assay (Sidekick) delivers repeatable results enabling on\u2010the\u2010spot decisions and reducing delays from sample processing, transport and result reporting, with potential to enable informed decision\u2010making on whether to initiate or continue treatment for a foal with FPT.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/evj.70172","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T05:07:07Z","timestamp":1777439227000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Validation of a stall\u2010side immunoglobulin assay for use in equine reproductive management"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-6457-9480","authenticated-orcid":false,"given":"L.","family":"Moore","sequence":"first","affiliation":[{"name":"Future Medicines Institute Centre for Digital Health Technology, Ulster University Belfast UK"},{"name":"Department of Biology University of York York UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6252-4693","authenticated-orcid":false,"given":"A.","family":"McLain","sequence":"additional","affiliation":[{"name":"Future Medicines Institute Centre for Digital Health Technology, Ulster University Belfast UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1886-9831","authenticated-orcid":false,"given":"S. H.","family":"McDowell","sequence":"additional","affiliation":[{"name":"Future Medicines Institute Centre for Digital Health Technology, Ulster University Belfast UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. J.","family":"Ledgerwood","sequence":"additional","affiliation":[{"name":"Future Medicines Institute Centre for Digital Health Technology, Ulster University Belfast UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8834-7058","authenticated-orcid":false,"given":"E.","family":"Bailie","sequence":"additional","affiliation":[{"name":"Future Medicines Institute Centre for Digital Health Technology, Ulster University Belfast UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7222-4124","authenticated-orcid":false,"given":"M. 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