{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T03:08:20Z","timestamp":1788750500966,"version":"build-2803163510"},"reference-count":36,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T00:00:00Z","timestamp":1788652800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T00:00:00Z","timestamp":1788652800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100022110","name":"bioM\u00e9rieux","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100022110","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n Insulin dysregulation (ID) and pituitary pars intermedia dysfunction (PPID) are common equine endocrinopathies detected by measurement of circulating insulin and adrenocorticotrophic hormone (ACTH) concentrations. Different assays may yield variable results, limiting comparability.<\/jats:p>\n <\/jats:sec>\n \n Objectives<\/jats:title>\n To compare immunoassays developed specifically to measure equine insulin (VIDAS\u00ae Equine Insulin; enzyme\u2010linked fluorescent assay [ELFA]) and equine ACTH (VIDAS\u00ae Equine ACTH; ELFA) with results obtained from a chemiluminescence immunoassay (CLIA) measured using IMMULITE 2000XPi, a chemiluminescence enzyme immunoassay (CLEIA) measured using TOSOH Automated Immunoassay Analyser\u2010360, and an equine insulin enzyme\u2010linked immunosorbent assay (ELISA; Mercodia).<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Residual serum (\n n<\/jats:italic>\n \u2009=\u2009242) and plasma (\n n<\/jats:italic>\n \u2009=\u2009337) from samples submitted to a commercial laboratory were simultaneously assayed using the CLIA and ELFA as appropriate. Concentrations were also measured in a subset using CLEIA (\n n<\/jats:italic>\n \u2009=\u2009126 insulin;\n n<\/jats:italic>\n \u2009=\u200976 ACTH) and ELISA (\n n<\/jats:italic>\n \u2009=\u200976 insulin) assays. Additionally, plasma [ACTH] was measured using the ELFA in samples from 139 healthy horses.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The median (95% confidence interval) difference for insulin assays was 1.6 (0.1\u20133.3)\u2009\u03bcIU\/mL for CLIA\u2013ELFA, 11.3 (8.7\u201313.4)\u2009\u03bcIU\/mL for CLEIA\u2013ELFA, 3.7 (1.0\u20136.3)\u2009\u03bcIU\/mL for ELISA\u2013ELFA, 7.3 (5.1\u20139.5)\u2009\u03bcIU\/mL for CLIA\u2013CLEIA; and for ACTH assays was 19.7 (16.8\u201325.1)\u2009pg\/mL for CLIA\u2013ELFA, 10.7 (6.9\u201315.5)\u2009pg\/mL for CLEIA\u2013ELFA, 23.7 (9.0\u201342.5)\u2009pg\/mL for CLIA\u2013CLEIA. Correlation was strong (\n R<\/jats:italic>\n \u2009>\u20090.8) or very strong (\n R<\/jats:italic>\n \u2009>\u20090.9) between pairs of insulin assays and between ELFA and CLEIA ACTH assays, and moderate (\n R<\/jats:italic>\n \u2009>\u20090.6) between other ACTH assay pairs. Bland\u2013Altman analysis revealed moderate bias and wide 95% limits of agreement between all assay pairs. Three\u2010way classification using adapted diagnostic cut\u2010offs for [insulin] and [ACTH] measured using ELFA indicated substantial agreement. A reference range for plasma [ACTH] in healthy horses was determined.\n <\/jats:p>\n <\/jats:sec>\n \n Main Limitations<\/jats:title>\n Residual samples were collected between July and November and from healthy horses but not ponies.<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n The assays yielded different absolute values, but agreement between most assay pairs and for three\u2010way classification for PPID diagnosis and laminitis risk was good.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/evj.70329","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T02:45:22Z","timestamp":1788749122000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of a new immunoassay for measuring equine serum insulin and plasma adrenocorticotrophic hormone: A method comparison 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