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The top two abundant aptamers, Cornea\u2010S1 and Cornea\u2010S2, could bind to pig cornea, and their <jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub> values to human corneal epithelial cells (HCECs) were 361 and 174 n\u043c, respectively. Aptamer\u2010functionalized liposomes loaded with cyclosporine A (CsA) were developed as a treatment for dry eye diseases. The <jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub> of Cornea\u2010S1\u2010 or Cornea\u2010S2\u2010functionalized liposomes reduces to 1.2 and 15.1 n\u043c, respectively, due to polyvalent binding. In HCECs, Cornea\u2010S1 or Cornea\u2010S2 enhanced liposome uptake within 15 min and extended retention to 24\u00a0h. Aptamer CsA liposomes achieved similar anti\u2010inflammatory and tight junction modulation effects with ten times less CsA than a free drug. In a rabbit dry eye disease model, Cornea\u2010S1 CsA liposomes demonstrated equivalence in sustaining corneal integrity and tear break\u2010up time when compared to commercial CsA eye drops while utilizing a lower dosage of CsA. The aptamers obtained from cornea\u2010SELEX can serve as a general ligand for ocular drug delivery, suggesting a promising avenue for the treatment of various eye diseases and even other diseases.<\/jats:p>","DOI":"10.1002\/exp.20230008","type":"journal-article","created":{"date-parts":[[2024,2,9]],"date-time":"2024-02-09T08:23:56Z","timestamp":1707467036000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Cornea\u2010SELEX for aptamers targeting the surface of eyes and liposomal drug delivery"],"prefix":"10.1002","volume":"4","author":[{"given":"Ka\u2010Ying","family":"Wong","sequence":"first","affiliation":[{"name":"Department of Chemistry, Waterloo Institute for Nanotechnology University of Waterloo  Waterloo Ontario Canada"},{"name":"Centre for Eye and Vision Research (CEVR) 17 W Hong Kong Science Park  Hong Kong Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yibo","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Waterloo Institute for Nanotechnology University of Waterloo  Waterloo Ontario Canada"},{"name":"Centre for Eye and Vision Research (CEVR) 17 W Hong Kong Science Park  Hong Kong Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man\u2010Sau","family":"Wong","sequence":"additional","affiliation":[{"name":"Centre for Eye and Vision Research (CEVR) 17 W Hong Kong Science Park  Hong Kong Hong Kong"},{"name":"Department of Food Science and Nutrition The Hong Kong Polytechnic University  Hung Hom, Kowloon Hong Kong"},{"name":"Research Center for Chinese Medicine Innovation The Hong Kong Polytechnic University  Hung Hom, Kowloon Hong Kong SAR P. 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