{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T20:33:58Z","timestamp":1784666038158,"version":"3.55.0"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T00:00:00Z","timestamp":1758240000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82474265"],"award-info":[{"award-number":["82474265"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82074241"],"award-info":[{"award-number":["82074241"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82204426"],"award-info":[{"award-number":["82204426"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Exploration"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Ulcerative colitis (UC) is a chronic and persistent clinical condition that is challenging to cure. Lysine crotonylation (KCr), a recently discovered post\u2010translational modification (PTM), alters protein structure, stability, localization and activity in a variety of processes including cell differentiation and organism development. This study was designed to elucidate the pathophysiological relevance of KCr in UC and uncover potential underlying mechanisms involved. PTM proteomics was employed to track dynamic alterations in KCr sites and protein level in the colon tissue of dextran sulfate sodium (DSS)\u2010induced UC model mice. Following the validation of differentially crotonylated proteins via Western blot assay, functional and mechanistic analyses of specific KCr sites were conducted in vitro. Gain\u2010of\u2010function or loss\u2010of\u2010function mutations were implemented at selected protein KCr sites. The differentially crotonylated proteins including citrate synthetase (CS) between the colon tissue of DSS\u2010induced mice and control mice were predominantly associated with the tricarboxylic acid (TCA) cycle, as evidenced by significant enrichment in the KEGG pathway analysis. These proteins were primarily localized in mitochondria, suggesting a potential link among UC pathogenesis, mitochondria and the TCA cycle. Collectively, increased KCr restricts inflammasome activation by inducing mitophagy, thereby maintaining mitochondrial homeostasis, reducing oxidative stress and inhibiting apoptosis in UC. KCr represents a potential promising therapeutic target for the treatment of UC.<\/jats:p>","DOI":"10.1002\/exp.20240129","type":"journal-article","created":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T07:25:38Z","timestamp":1758266738000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Modulating Lysine Crotonylation in Ulcerative Colitis Maintains Mitochondrial Homeostasis"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1764-5521","authenticated-orcid":false,"given":"Tongtong","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Pharmacy Affiliated Hospital of Nanjing University of Chinese Medicine Jiangsu Province Hospital of Chinese Medicine Nanjing China"},{"name":"College of The First Clinical Medicine Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binyan","family":"Lin","sequence":"additional","affiliation":[{"name":"Laboratory of Herbal Active Compound Omics and Target Identification, School of Pharmacy Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Affiliated Hospital of Nanjing University of Chinese Medicine Jiangsu Province Hospital of Chinese Medicine Nanjing China"},{"name":"College of The First Clinical Medicine Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayu","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Affiliated Hospital of Nanjing University of Chinese Medicine Jiangsu Province Hospital of Chinese Medicine Nanjing China"},{"name":"College of The First Clinical Medicine Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaochao","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Affiliated Hospital of Nanjing University of Chinese Medicine Jiangsu Province Hospital of Chinese Medicine Nanjing China"},{"name":"College of The First Clinical Medicine Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Affiliated Hospital of Nanjing University of Chinese Medicine Jiangsu Province Hospital of Chinese Medicine Nanjing China"},{"name":"College of The First Clinical Medicine Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E\u2010Hu","family":"Liu","sequence":"additional","affiliation":[{"name":"Laboratory of Herbal Active Compound Omics and Target Identification, School of Pharmacy Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijia","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Affiliated Hospital of Nanjing University of Chinese Medicine Jiangsu Province Hospital of Chinese Medicine Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,19]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)60150-0"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/ibd\/izab346"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mayocp.2019.01.018"},{"issue":"1","key":"e_1_2_11_5_1","first-page":"5","article-title":"Relationship Between Intestinal Microbiota and Ulcerative Colitis: Mechanisms and Clinical Application of Probiotics and Fecal Microbiota Transplantation","volume":"24","author":"Shen Z. H.","year":"2018","journal-title":"Gastroenterology"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.20805.1"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.998470"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.7861\/clinmed.2021-0080"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13402-022-00740-2"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2022.106530"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2018.04.012"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.107.113613"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adf0138"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.161096"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2022.101817"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2021.12.003"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-018-0081-3"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2022.01.006"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.495"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14650"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13578-021-00616-2"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.58872"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.08.008"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2022.104488"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2020.10.259"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.122.321054"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2021.105796"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2021.118231"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells8030283"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2022.2084862"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2022.102469"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.129118"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12774"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-022-04786-w"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2101-7"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2021.10.002"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2022.01.006"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2022.0240"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2013.10.021"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-021-03987-z"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09663"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2023.2166276"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2020.01.152"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-06061-0"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-022-04725-9"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.115108"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2023.102613"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-022-03782-0"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2014.06.020"},{"issue":"29","key":"e_1_2_11_50_1","article-title":"TRIM21\u2010Promoted FSP1 Plasma Membrane Translocation Confers Ferroptosis Resistance in Human Cancers","volume":"10","author":"Gong J.","year":"2023","journal-title":"Advancement of Science"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20170856"}],"container-title":["Exploration"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/EXP.20240129","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T21:19:58Z","timestamp":1767043198000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/EXP.20240129"}},"subtitle":["Modulating Crotonylation in Ulcerative Colitis"],"short-title":[],"issued":{"date-parts":[[2025,9,19]]},"references-count":50,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1002\/EXP.20240129"],"URL":"https:\/\/doi.org\/10.1002\/exp.20240129","archive":["Portico"],"relation":{},"ISSN":["2766-8509","2766-2098"],"issn-type":[{"value":"2766-8509","type":"print"},{"value":"2766-2098","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,19]]},"assertion":[{"value":"2024-06-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"20240129"}}