{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T17:01:01Z","timestamp":1781715661203,"version":"3.54.5"},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,5,4]],"date-time":"2025-05-04T00:00:00Z","timestamp":1746316800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31970112"],"award-info":[{"award-number":["31970112"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170109"],"award-info":[{"award-number":["32170109"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["41907211"],"award-info":[{"award-number":["41907211"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Exploration"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Bronchiectasis frequently co\u2010exists with chronic obstructive pulmonary disease (COPD\u2010bronchiectasis association [CBA]). We compared the microbiota and metabolome of bronchiectasis with (BO) and without airflow obstruction (BNO), COPD, and CBA. We determined how microbiota compositions correlated with clinical characteristics and exacerbations of CBA. We prospectively recruited outpatients with BNO (<jats:italic>n<\/jats:italic> = 104), BO (<jats:italic>n<\/jats:italic> = 51), COPD (<jats:italic>n<\/jats:italic> = 33), and CBA (<jats:italic>n<\/jats:italic> = 70). We sampled at stead\u2010state and exacerbation, and profiled sputum microbiota via 16S rRNA sequencing and metabolome via liquid chromatography\/mass spectrometry. Sputum microbiota and metabolome profiles of CBA separated from COPD (<jats:italic>P<\/jats:italic> &lt; 0.05) but not bronchiectasis, partly driven by Proteobacteria enrichment in CBA. An increasing microbial interaction but not microbiota compositions were identified at exacerbation. Pseudomonadaceae\u2010dominant CBA yielded lower Shannon\u2013Wiener diversity index (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001), greater bronchiectasis severity (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) and higher future exacerbation risk (HR 2.46, 95% CI: 1.34\u20134.52, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001) than other genera\u2010dominant CBA. We found a clear metabolite discrimination between CBA and COPD. Most of up\u2010regulated metabolites identified in CBA, were amino acids metabolites, which indicated that the accumulation of amino acids metabolites was related to the alteration of airway microbiota. To conclude, airway structural changes, but not airflow limitation, correlate more profoundly with microbiota and metabolome profiles (e.g. partly via Pseudomonadaceae\u2010amino acids metabolism links), shaping clinical outcomes of CBA.<\/jats:p>","DOI":"10.1002\/exp.20240149","type":"journal-article","created":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T00:50:15Z","timestamp":1746406215000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Sputum Microbiota Compositions Correlate With Metabolome and Clinical Outcomes of COPD\u2010Bronchiectasis Association: A Prospective Cohort Study"],"prefix":"10.1002","volume":"5","author":[{"given":"Zhen\u2010feng","family":"He","sequence":"first","affiliation":[{"name":"Department of Allergy and Clinical Immunology Department of Respiratory and Critical Care Medicine State Key Laboratory of Respiratory Diseases National Clinical Research Center for Respiratory Disease National Center for Respiratory Medicine Guangzhou Institute of Respiratory Health First Affiliated Hospital Guangzhou Medical University  Guangzhou Guangdong P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010xian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Allergy and Clinical Immunology Department of Respiratory and Critical Care Medicine State Key Laboratory of Respiratory Diseases National Clinical Research Center for Respiratory Disease National Center for Respiratory Medicine Guangzhou Institute of Respiratory Health First Affiliated Hospital Guangzhou Medical University  Guangzhou Guangdong P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cui\u2010xia","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Allergy and Clinical Immunology Department of Respiratory and Critical Care Medicine State Key Laboratory of Respiratory Diseases National Clinical Research Center for Respiratory Disease National Center for Respiratory Medicine Guangzhou Institute of Respiratory Health First Affiliated Hospital Guangzhou Medical University  Guangzhou Guangdong P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin\u2010zhu","family":"Yi","sequence":"additional","affiliation":[{"name":"Institute of Ecological Sciences School of Life Sciences South China Normal University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Geriatrics National Key Clinical Specialty Guangzhou First People's Hospital South China University of Technology  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun\u2010lan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Guangdong Provincial People's Hospital Guangdong Academy of Medical Sciences  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan\u2010shan","family":"Zha","sequence":"additional","affiliation":[{"name":"Department of Allergy and Clinical Immunology Department of Respiratory and Critical Care Medicine State Key Laboratory of Respiratory Diseases National Clinical Research Center for Respiratory Disease National Center for Respiratory Medicine Guangzhou Institute of Respiratory Health First Affiliated Hospital Guangzhou Medical University  Guangzhou Guangdong P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lai\u2010jian","family":"Cen","sequence":"additional","affiliation":[{"name":"Department of Allergy and Clinical Immunology Department of Respiratory and Critical Care Medicine State Key Laboratory of Respiratory Diseases National Clinical Research Center for Respiratory Disease National Center for Respiratory Medicine Guangzhou Institute of Respiratory Health First Affiliated Hospital Guangzhou Medical University  Guangzhou Guangdong P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han\u2010qin","family":"Cai","sequence":"additional","affiliation":[{"name":"Institute of Ecological Sciences School of Life Sciences South China Normal University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Ecological Sciences School of Life Sciences South China Normal University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia\u2010qi","family":"Gao","sequence":"additional","affiliation":[{"name":"Institute of Ecological Sciences School of Life Sciences South China Normal University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui\u2010min","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Allergy and Clinical Immunology Department of Respiratory and Critical Care Medicine State Key Laboratory of Respiratory Diseases National Clinical Research Center for Respiratory Disease National Center for Respiratory Medicine Guangzhou Institute of Respiratory Health First Affiliated Hospital Guangzhou Medical University  Guangzhou Guangdong P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen\u2010hong","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Allergy and Clinical Immunology Department of Respiratory and Critical Care Medicine State Key Laboratory of Respiratory Diseases National Clinical Research Center for Respiratory Disease National Center for Respiratory Medicine Guangzhou Institute of Respiratory Health First Affiliated Hospital Guangzhou Medical University  Guangzhou Guangdong P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng\u2010zhu","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Allergy and Clinical Immunology Department of Respiratory and Critical Care Medicine State Key Laboratory of Respiratory Diseases National Clinical Research Center for Respiratory Disease National Center for Respiratory Medicine Guangzhou Institute of Respiratory Health First Affiliated Hospital Guangzhou Medical University  Guangzhou Guangdong P.R. 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