{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T05:16:07Z","timestamp":1783055767689,"version":"3.54.6"},"reference-count":78,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T00:00:00Z","timestamp":1773878400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T00:00:00Z","timestamp":1773878400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82302900"],"award-info":[{"award-number":["82302900"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2024A1515030104"],"award-info":[{"award-number":["2024A1515030104"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Exploration"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Hepatitis B virus (HBV) infection poses a significant challenge to global health, particularly in developing countries such as China, where HBV\u2010related acute liver failure (HBV\u2010ALF) is a prominent cause of acute liver failure. This study investigated the effect of cuproptosis, a recently identified form of cell death, on immune infiltration in HBV\u2010ALF. We mined the gene expression data of HBV\u2010ALF from the Gene Expression Omnibus database. Through enrichment analysis of differentially expressed genes (DEGs), pathways related to the response to metal\/copper ions and the acute inflammatory response were found to be enriched. We subsequently found that HBV\u2010ALF tissues contained more copper ions and conducted an intersection analysis of DEGs and cuproptosis\u2010related genes (CRGs), which resulted in the identification of 7 core cuproptosis\u2010related DEGs (CR\u2010DEGs) for further investigation with a diagnostic model. Immune infiltration analysis and unsupervised clustering analysis revealed distinct patterns in HBV\u2010ALF and the possibility of crosstalk between ferroptosis and cuproptosis. Furthermore, we identified 17 transcription factors, 90 miRNAs, and 15 drugs that might interact with the 7 CR\u2010DEGs. To validate our findings and their clinical significance, we verified the diagnostic value and immune infiltration patterns of the 7 CR\u2010DEGs in both the testing dataset, cell line, and clinical samples. In conclusion, our findings indicated that these 7 CR\u2010DEGs demonstrate promising diagnostic value and may represent viable therapeutic targets for individuals with HBV\u2010ALF.<\/jats:p>","DOI":"10.1002\/exp.20240267","type":"journal-article","created":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T04:14:18Z","timestamp":1773980058000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cuproptosis\u2010Related Genes in Immune Infiltration and Diagnosis in Hepatitis B Virus\u2010Related Acute Liver Failure"],"prefix":"10.1002","volume":"6","author":[{"given":"Jingwen","family":"Deng","sequence":"first","affiliation":[{"name":"Department of Medical Oncology Sir Run Run Shaw Hospital School of Medicine Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Pathology Sir Run Run Shaw 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Druggability Assessment Guangdong Basic Research Center of Excellence for Natural Bioactive Molecules and Discovery of Innovative Drugs College of Pharmacy Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiqiang","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology West German Cancer Center University Hospital Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2118-8888","authenticated-orcid":false,"given":"Chao","family":"Lu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioactive Molecules and Druggability Assessment Guangdong Basic Research Center of Excellence for Natural Bioactive Molecules and Discovery of Innovative Drugs College of Pharmacy Jinan University  Guangzhou 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