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However, present gold\u2010standard testing methods based on nucleic acid amplification are limited by their time and labor\u2010intensive nature. Hence, we reported an amplification\u2010free assay that facilitates rapid, sensitive, and point\u2010of\u2010care detection of infectious diseases. Herein, spherical nucleic acid (SNA)\u2010stabilized cage\u2010type three\u2010dimensional electrochemiluminescence (ECL) reporter probe, constructed utilizing Ru(dcbpy)\n                    <jats:sub>3<\/jats:sub>\n                    Cl\n                    <jats:sub>2<\/jats:sub>\n                    \u2010doped NH\n                    <jats:sub>2<\/jats:sub>\n                    \u2010MIL\u2010101 (Fe) metal\u2010organic framework, was combined with a paper\u2010based bipolar electrode ECL sensor. Furthermore, surface modifications were performed using polydopamine and SNAs to further improve the stability. Notably, compared with the ordinary Ru(dcbpy)\n                    <jats:sub>3<\/jats:sub>\n                    Cl\n                    <jats:sub>2<\/jats:sub>\n                    , the modified ECL probe attained 10\n                    <jats:sup>3<\/jats:sup>\n                    \u2010fold enhancement in luminous efficiency. The developed ECL\u2010based nucleic acid biosensing system offers the advantages of simplicity, portability, low cost, and user\u2010friendly nucleic acid detection, with a sample\u2010to\u2010answer time of approximately 15\u00a0min. Additionally, the effectiveness of the developed assay in detecting pathogenic nucleic acid was assessed using Mpox and SARS\u2010Cov\u20102, exhibiting a wide dynamic range from 33 to 10\n                    <jats:sup>10<\/jats:sup>\n                    aM and limits of detection as low as 3 copies\/\u00b5L. Furthermore, the assay exhibited 100% sensitivity and specificity when validated against quantitative polymerase chain reaction\u2010based detection using clinical samples. Altogether, the findings of this study show the efficacy of the novel developed SNA\u2010stabilized cage\u2010type 3D ECL probe\u2010enhanced nucleic acid assay, along with its potential as a promising paradigm for point\u2010of\u2010care diagnosis of infectious pathogens.\n                  <\/jats:p>","DOI":"10.1002\/exp.20240451","type":"journal-article","created":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T07:36:12Z","timestamp":1773473772000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Spherical Nucleic Acid Stabilized Cage Type Three\u2010Dimensional Electrochemiluminescence Probe for Sensitive and Amplification\u2010Free Diagnosis of Infectious Diseases"],"prefix":"10.1002","volume":"6","author":[{"given":"Yu","family":"Fu","sequence":"first","affiliation":[{"name":"NHC Key Laboratory of Metabolic Cardiovascular Diseases Research, Ningxia Key Laboratory of Vascular Injury and Repair Research 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