{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T04:18:18Z","timestamp":1787804298618,"version":"build-2784847793"},"reference-count":65,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T00:00:00Z","timestamp":1779062400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T00:00:00Z","timestamp":1779062400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Exploration"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The construction of an optimized biosensing interface is critically important for electrochemical biosensing. Herein, we achieve the controllable construction of macroporous covalent organic framework (COF) nanostructures through a template\u2010assisted assembly growth strategy, resulting in well\u2010organized morphologies that are well\u2010suited as advanced electrode materials. These macroporous structures with unique pore microenvironments support efficient immobilization of biorecognition elements via structure\u2010confined supramolecular interactions. Notably, the 3D hierarchically macroporous COF superstructure enables the creation of an improved biosensing interface, facilitating enzyme immobilization, enhanced mass and charge transfer, and efficient substrate enrichment, thereby significantly boosting the biosensing performance. Consequently, the developed biosensing electrode loaded with acetylcholinesterase (AChE) displays high sensitivity, stability, and strong anti\u2010interference capability for the electrochemical detection of diverse organophosphorus pesticides (OPs), with the ultralow limits of detection at a sub\u2010pg mL\n                    <jats:sup>\u20121<\/jats:sup>\n                    level. Moreover, a portable biosensing device integrated with AChE\u2010loaded HMSCOF demonstrates reliable recovery levels (ranging from 96.4 to 105.3%) for OP detection in river water and leafy vegetables, underscoring its practical applicability. This study demonstrates that the amalgamation of macroporous COF\u2010based materials with biorecognition elements offers a promising strategy for the advancement\u00a0of high\u2010performance portable electrochemical biosensors intended for environmental monitoring and food safety applications.\n                  <\/jats:p>","DOI":"10.1002\/exp2.70141","type":"journal-article","created":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T16:41:27Z","timestamp":1779122487000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Customizing 3D Hierarchically Macroporous Covalent Organic Framework Superstructures for Improved Biosensing Interface in Highly\u2010Sensitive Electrochemical Biosensors"],"prefix":"10.1002","volume":"6","author":[{"given":"Wenbo","family":"Wei","sequence":"first","affiliation":[{"name":"School of Materials Science and Engineering Zhejiang Sci\u2010Tech University Hangzhou China"},{"name":"State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences (CAS) Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng\u2010Hua","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering Jiangsu University of Technology Changzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong\u2010Dong","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences (CAS) Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences (CAS) Fuzhou 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