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However, the mechanisms by which rumen fungi influence the susceptibility and tolerance of ruminants to SARA remain unclear. This study investigated the relationship between rumen fungal metabolic functions and host SARA tolerance by examining the rumen epithelial miRNA\u2010mRNA correlations and further cell subtype variations affected by fungi. SARA\u2010susceptible and SARA\u2010tolerant goats were identified by monitoring the dynamic rumen pH during high\u2010concentrate feeding. The abundance of anaerobic fungi and their cellulose and amino acid metabolic functions were reduced in SARA\u2010susceptible goats, but the enrichment of\n                    <jats:italic>Aspergillus bombycis<\/jats:italic>\n                    , which is associated with increased thiamine metabolism, offers potential for SARA tolerance. Furthermore, this study revealed that\n                    <jats:italic>Aspergillus bombycis<\/jats:italic>\n                    negatively regulates the rumen epithelial chi\u2010miR\u201017\u201026080 through enhanced thiamine metabolism, thereby reducing its inhibitory effect on insulin\u2010like growth factor binding protein 2 (IGFBP2). The tolerance mechanism of SARA was further explored by adding thiamine to high concentrations, which revealed that the upregulation of IGFBP2 promoted rumen epithelial IGF1 recruitment and increased the content of IGF1, which activated the ERK and PI3K\/AKT pathways, further increasing the expression of CDK4 and Cyclin A2 and promoting the rumen epithelial cells' proliferation. The increased number of rumen spinous and basal cells in SARA\u2010tolerant goats enhances volatile fatty acids absorption in dairy goats, leading to improved SARA tolerance. These findings highlight the roles of rumen fungi and their produced thiamine in preventing SARA in ruminants by enhancing epithelial cell proliferation via the IGFBP2\/IGF1 axis activation.\n                  <\/jats:p>","DOI":"10.1002\/exp2.70142","type":"journal-article","created":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T03:39:26Z","timestamp":1773027566000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Rumen Mycobiome Thiamine Metabolism Contributes to Subacute Rumen Acidosis Tolerance in Goats Through Enhancing Epithelial Cell Proliferation via IGFBP2\/IGF1 Axis Activation"],"prefix":"10.1002","volume":"6","author":[{"given":"Jingyi","family":"Xu","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University  Yangling Shaanxi China"},{"name":"Key Laboratory of Livestock Biology Northwest A&amp;F University  Yangling Shaanxi 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