{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T04:47:16Z","timestamp":1791866836509,"version":"4.3.4"},"reference-count":45,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["L232087"],"award-info":[{"award-number":["L232087"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32271443"],"award-info":[{"award-number":["32271443"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Exploration"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Despite significant advances in nanovaccine development, their efficacy remains hindered by poor antigen presentation, limited antigen repertoire, and suboptimal T cell priming. Immune adjuvants such as polyinosinic\u2010polycytidylic acid (POLY(I:C)) exert antitumor effects by promoting dendritic cell (DC) maturation and stimulating innate immunity, but their efficacy is limited by poor antigen presenting cell (APC) uptake and weak CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell responses. Here, we propose a novel adjuvant strategy using nitric oxide (NO), a bioactive gaseous molecule, to upregulate MHC\u2010I and enhance DC cross\u2010presentation, thereby synergistically improving the adjuvant efficacy of POLY(I:C). We developed a nanovaccine, Im@NOnanoPL, by co\u2010encapsulating a preassembled complex of DOTAP, NOC\u201018, and POLY(I:C) into a PLGA shell, which was further modified with DSPE\u2010PEG\n                    <jats:sub>2000<\/jats:sub>\n                    and camouflaged with immunogenic cell death (ICD)\u2013derived tumor membranes for broad\u2010spectrum tumor antigen coverage. Im@NOnanoPL elicited robust and durable CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell responses, conferring near\u2010complete prophylactic protection and, in combination with \u03b1PD\u20101 therapy, eradicating established tumors while inducing long\u2010term immune memory. Im@NOnanoPL constitutes a novel vaccine platform that integrates a gas\u2010based adjuvant with broad\u2010spectrum antigens, exhibiting strong translational potential.\n                  <\/jats:p>","DOI":"10.1002\/exp2.70238","type":"journal-article","created":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T09:49:52Z","timestamp":1791712192000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Nitric Oxide\u2013Based STING Agonist Nanoadjuvant With ICD\u2010Derived Membrane Antigens Unleashes Robust Antitumor Immunity"],"prefix":"10.1002","author":[{"given":"Shuyu","family":"Xu","sequence":"first","affiliation":[{"name":"Beijing Key Laboratory of Ultrasound Targeted Drug Delivery and Frontier Imaging Technology, Department of Ultrasound Peking University Third Hospital Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Song","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Ultrasound Targeted Drug Delivery and Frontier Imaging Technology, Department of Ultrasound Peking University Third Hospital Beijing P. R. China"},{"name":"Department of Ultrasound Medicine, Beijing Chaoyang Hospital Capital Medical University Chaoyang Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianjiao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Ultrasound Targeted Drug Delivery and Frontier Imaging Technology, Department of Ultrasound Peking University Third Hospital Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Ultrasound The First Affiliated Hospital of China Medical University Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengxin","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Ultrasound Targeted Drug Delivery and Frontier Imaging Technology, Department of Ultrasound Peking University Third Hospital Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxin","family":"Xie","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Ultrasound Targeted Drug Delivery and Frontier Imaging Technology, Department of Ultrasound Peking University Third Hospital Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingfeng","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cell Biology Agency For Science, Technology and Research Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolong","family":"Liang","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Ultrasound Targeted Drug Delivery and Frontier Imaging Technology, Department of Ultrasound Peking University Third Hospital Beijing P. R. China"},{"name":"State Key Laboratory of Vascular Homeostasis and Remodeling, Beijing Key Laboratory of Interdisciplinary Research in Gastrointestinal Oncology (BLGO) Peking University Third Hospital Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573\u2010024\u201001081\u20105"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568\u2010025\u201000820\u2010z"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565\u2010025\u201001931\u20102"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.2c06560"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1001294"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2016.34.15_suppl.3084"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdz253.055"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2016.34.15_suppl.8519"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V128.22.4715.4715"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/cgt.2012.55"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiad295"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577\u2010023\u201000937\u2010y"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aar7112"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(23)02268\u20107"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159\u20108290.CD\u201024\u20100158"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010023\u201006063\u2010y"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591\u2010020\u201001206\u20104"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571\u2010024\u201000902\u20101"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943\u2010021\u201001335\u20105"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2024.05.005"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(25)00553\u20107"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.4c09566"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2025.113801"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41377\u2010024\u201001673\u20101"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2015.01.001"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2013.05.013"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40425\u2010019\u20100568\u20102"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2014.09.010"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202413392"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573\u2010024\u201000974\u20109"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2CS00647B"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adk2444"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202109254"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202007293"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202103790"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2017.140"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202210452"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.06.010"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589\u2010023\u201001411\u20105"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202001550"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.4c00654"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12951\u2010023\u201002031\u2010w"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2024.10.060"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acra.2025.03.034"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2015.10.002"}],"container-title":["Exploration"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/exp2.70238","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/exp2.70238","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/exp2.70238","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date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