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But the ideal AA pattern, especially the reasonable proportion of amino acids such as the desired methionine (Met): cystine (Cys) ratios are not yet clear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>In this article, the experiments of low\u2010protein diet with varying ratios of Met:Cys (low ratio of 1:3, medium ratio of 1:1, and high ratio of 3:1) were conducted to investigate the effect on mice growth and reproductive performance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The result indicated that the lower Met:Cys ratio improves reproductive performance in male mice, but the growth performance were no change in all groups. Meanwhile, the abnormality rate of sperm increased as the ratio of methionine and cystine increased. Quantitative\u00a0analysis\u00a0showed that the low Met:Cys ratio has obviously decreased the expression of Bax protein and the concentrations of testosterone in male serum, but Prm2, Pgk2, Bcl\u20102, Bak1, and AR gene were made no difference. Furthermore, different Met:Cys ratios have significant effects on the modulated methionine cycle by increasing the expressions of MAT2B, GNMT, and BHMT in low Met:Cys ratio. On the other hand, it was also found that there were increases in GSH\u2010Px enzyme activities and decreases in MDA levels in male serum.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Overall, the present study showed that a dietary lower Met:Cys ratio in a low\u2010protein diet had a positive influence on the reproductive performance of male animal through obviously improving sperm quality and antioxidant capacity, and\u00a0inhibiting apoptosis. And the study provided new pieces of evidence to re\u2010evaluate the role of precise sulfur amino acid nutrition in a low\u2010protein diet.<\/jats:p><\/jats:sec>","DOI":"10.1002\/fsn3.1128","type":"journal-article","created":{"date-parts":[[2019,8,12]],"date-time":"2019-08-12T02:53:41Z","timestamp":1565578421000},"page":"2866-2874","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Lower methionine\/cystine ratio in low\u2010protein diet improves animal reproductive performance by modulating methionine cycle"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5651-4014","authenticated-orcid":false,"given":"Xingping","family":"Chen","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, College of Animal Science South China Agricultural University Guangzhou 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