{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T12:58:44Z","timestamp":1784293124984,"version":"3.55.0"},"reference-count":24,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,11,14]],"date-time":"2019-11-14T00:00:00Z","timestamp":1573689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31501452"],"award-info":[{"award-number":["31501452"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81302781"],"award-info":[{"award-number":["81302781"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp; Nutrition"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>It has been reported that <jats:italic>Lactobacillus gasseri<\/jats:italic> PA3 has an ability to absorb exogenous purines in the intestine to reduce a risk of gout and hyperuricemia. However, influences of this strain on gut microbiota and their metabolisms remain unclear. Herein, we aimed to investigate the effect of <jats:italic>L.\u00a0gasseri<\/jats:italic> PA3 on microbiota composition and metabolisms. <jats:italic>L.\u00a0gasseri<\/jats:italic> PA3 was isolated from yogurt and supplemented into a single\u2010stage colonic fermentation in a culture volume of 30\u00a0ml and subjected to in vitro colonic simulation for 8\u00a0days. Microbiota composition was determined with 16S rRNA (V3\u00a0+\u00a0V4) sequencing, and their metabolisms were predicted by PICRUSt. Short\u2010chain fatty acids were measured by GC\u2010MS. We found that <jats:italic>L.\u00a0gasseri<\/jats:italic> PA3 reduced the diversity of microbiota, increased the relative abundances of <jats:italic>Lactobacillus<\/jats:italic> (73.5%) and <jats:italic>Escherichia<\/jats:italic> (36.5%), and decreased <jats:italic>Bacterioides<\/jats:italic> and <jats:italic>Phascolarctobacterium<\/jats:italic>. Total amount of short\u2010chain fatty acids was found to decline. Fundamental metabolisms, especially nucleotide, was significantly higher after intervention with <jats:italic>L.\u00a0gasseri<\/jats:italic> PA3, but the purine metabolism was lower, which means that PA3 might reduce uric acid concentrations by weakening purine metabolism. Our results indicated that <jats:italic>L.\u00a0gasseri<\/jats:italic> PA3 can survive and play a role in the ascending colon environment. Therefore, the evaluation of the effect of <jats:italic>L.\u00a0gasseri<\/jats:italic> PA3 on intestinal microbes and their metabolisms has great guiding significance for the development of treatment to prevent gout.<\/jats:p>","DOI":"10.1002\/fsn3.1236","type":"journal-article","created":{"date-parts":[[2019,11,14]],"date-time":"2019-11-14T16:55:28Z","timestamp":1573750528000},"page":"3883-3891","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Effect of <i>Lactobacillus gasseri<\/i> PA3 on gut microbiota in an in vitro colonic simulation"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2280-4707","authenticated-orcid":false,"given":"Shasha","family":"Xiang","sequence":"first","affiliation":[{"name":"School of Food Science and Bioengineering Zhejiang Gongshang University Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Fu","sequence":"additional","affiliation":[{"name":"Eurofins Technology Service Qingdao Co., Ltd Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Ye","sequence":"additional","affiliation":[{"name":"School of Food Science and Bioengineering Zhejiang Gongshang University Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3261-2939","authenticated-orcid":false,"given":"Yiqing","family":"Zheng","sequence":"additional","affiliation":[{"name":"School of Food Science and Bioengineering Zhejiang Gongshang University Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Zhu","sequence":"additional","affiliation":[{"name":"School of Food Science and Bioengineering Zhejiang Gongshang University Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Food Science and Bioengineering Zhejiang Gongshang University Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuewen","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Food Science and Bioengineering Zhejiang Gongshang University Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,11,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0407076101"},{"issue":"03","key":"e_1_2_9_3_1","first-page":"169","article-title":"Directed mutagenesis of Lactobacillus bulgaricus for decomposition of uremic toxins","volume":"13","author":"Bai Y. 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