{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T04:08:09Z","timestamp":1790482089449,"version":"4.1.0"},"reference-count":61,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,12,23]],"date-time":"2019-12-23T00:00:00Z","timestamp":1577059200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp; Nutrition"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This research describes an investigation of the antipyretic and hepatoprotective properties of both a crude organic extract and various subfractions of the ethnomedicinal plant\n                    <jats:italic>Tinospora crispa<\/jats:italic>\n                    , using appropriate animal models. In an attempt to identify potential lead hepatoprotective compounds, in silico experiments were utilized. Antipyretic activity was assessed via the Brewer's yeast\u2010induced pyrexia method, while hepatoprotective effects were evaluated in a carbon tetrachloride (CCl\n                    <jats:sub>4<\/jats:sub>\n                    )\u2010induced animal model. A computer\u2010aided prediction of activity spectra for substances (PASS) model was applied to a selection of documented phytoconstituents, with the aim of identifying those compounds with most promising hepatoprotective effects. Results were analyzed using Molinspiration software. Our results showed that both the methanol extract (METC) and various subfractions (pet ether, PEFTC;\n                    <jats:italic>n<\/jats:italic>\n                    \u2010hexane, NHFTC; and chloroform, CFTC) significantly (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.05) reduced pyrexia in a dose\u2010dependent manner. In CCl\n                    <jats:sub>4<\/jats:sub>\n                    \u2010induced hepatotoxicity studies, METC ameliorated elevated hepatic markers including serum alanine amino transferase (ALT), aspartate amino transferase (AST), alkaline phosphatase (ALP), and total bilirubin. Malondialdehyde (MDA) levels were significantly reduced, while superoxide dismutase (SOD) levels were significantly increased. Among a selection of metabolites of\n                    <jats:italic>T.\u00a0crispa<\/jats:italic>\n                    , genkwanin was found to be the most potent hepatoprotective constituent using PASS predictive models. These results demonstrate that both the methanolic extract of\n                    <jats:italic>T.\u00a0crispa<\/jats:italic>\n                    and those fractions containing genkwanin may offer promise in reducing pyrexia and as a source of potential hepatoprotective agents.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.1339","type":"journal-article","created":{"date-parts":[[2019,12,23]],"date-time":"2019-12-23T14:15:41Z","timestamp":1577110541000},"page":"547-556","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":71,"title":["Antipyretic and hepatoprotective potential of\n                    <i>Tinospora crispa<\/i>\n                    and investigation of possible lead compounds through in silico approaches"],"prefix":"10.1002","volume":"8","author":[{"given":"Ahmed","family":"Rakib","sequence":"first","affiliation":[{"name":"Department of Pharmacy Faculty of Biological Science University of Chittagong Chittagong Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahriar","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Faculty of Biological Science University of Chittagong Chittagong Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Ashiqul","family":"Islam","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Faculty of Biological Science University of Chittagong Chittagong Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul","family":"Haye","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine University of Science and Technology Chittagong Chittagong Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. M. Naim","family":"Uddin","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Faculty of Biological Science University of Chittagong Chittagong Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mir Muhammad Nasir","family":"Uddin","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Faculty of Biological Science University of Chittagong Chittagong Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed Kamrul","family":"Hossain","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Faculty of Biological Science University of Chittagong Chittagong Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arkajyoti","family":"Paul","sequence":"additional","affiliation":[{"name":"Drug Discovery GUSTO A Research Group Chittagong Bangladesh"},{"name":"Department of Microbiology Jagannath University Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3188-2272","authenticated-orcid":false,"given":"Talha Bin","family":"Emran","sequence":"additional","affiliation":[{"name":"Drug Discovery GUSTO A Research Group Chittagong Bangladesh"},{"name":"Department of Pharmacy BGC Trust University Bangladesh Chittagong Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,12,23]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2016.00059"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40816-019-0127-x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1515\/jbcpp-2015-0056"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.2131\/jts.27.139"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13596-019-00380-2"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-957466"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2008.06.020"},{"key":"e_1_2_10_9_1","volume-title":"Studies on the constituents of the dry stem of Tinospora crispa (Lour.) 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