{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:57:44Z","timestamp":1787317064303,"version":"build-2736575974"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,2,24]],"date-time":"2020-02-24T00:00:00Z","timestamp":1582502400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"This study was received by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education","award":["NRF-2017R1D1A1B03035934"],"award-info":[{"award-number":["NRF-2017R1D1A1B03035934"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp; Nutrition"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Orostachys japonicus<\/jats:italic>\n                    (\n                    <jats:italic>O.\u00a0japonicus<\/jats:italic>\n                    ) was extracted with ethanol (EtOH) and sequentially separated with organic solvents, including n\u2010hexane (Hex), dichloromethane (DCM), ethyl acetate (EtOAc), n\u2010butanol (BuOH), and water (H\n                    <jats:sub>2<\/jats:sub>\n                    O). All the fractions were confirmed for anti\u2010inflammatory activity in an inflammatory condition. The DCM fraction showed the highest anti\u2010inflammatory ability. Here, we examined the effect of DCM fraction and investigated the intracellular signaling pathways in LPS\u2010stimulated RAW 264.7 macrophage cells. The DCM fraction significantly inhibited the mRNA levels of pro\u2010inflammatory mediators and cytokines including iNOS, COX\u20102, IL\u20101\u03b2, IL\u20102, IL\u20106, and IP\u201010 in LPS\u2010stimulated cells. Also, the treatment of DCM fraction excellently reduced the expression of the proteins of AP\u20101 (phospho\u2010c\u2010Jun and phospho\u2010c\u2010Fos) and phospho\u2010IRF3 as transcription factors. As a result, it suppressed LPS\u2010induced inflammatory mediator and cytokines via inhibition of transcription factors. In conclusion, our data demonstrated that DCM fraction has a strong anti\u2010inflammatory activity that improves the inflammatory state.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.1441","type":"journal-article","created":{"date-parts":[[2020,2,24]],"date-time":"2020-02-24T05:09:35Z","timestamp":1582520975000},"page":"1812-1817","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<i>Orostachys japonicus<\/i>\n                    extract inhibits the lipopolysaccharide\u2010induced pro\u2010inflammatory factors by suppression of transcription factors"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7732-7149","authenticated-orcid":false,"given":"Hyeong\u2010Seon","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Biomedical Laboratory Science Jungwon University Goesan South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,2,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114512005594"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni921"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.01.025"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24071236"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.1207"},{"key":"e_1_2_8_7_1","first-page":"51","article-title":"Anti\u2010hyperlipidemic effects of the flavonoid\u2010rich fraction from the methanol extract of Orostachys japonicus in rats","volume":"40","author":"Kim S. 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