{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T04:38:59Z","timestamp":1784608739763,"version":"3.55.0"},"reference-count":52,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,2,20]],"date-time":"2020-02-20T00:00:00Z","timestamp":1582156800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010740","name":"Hasanuddin University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100010740","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp; Nutrition"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this research was to determine the optimal medium for Exopolysaccharides (EPS) production by a <jats:italic>Lactobacillus delbrueckii<\/jats:italic> subsp. <jats:italic>bulgaricus<\/jats:italic> ropy strain isolated from a locally produced commercial fermented milk, in reconstituted skim milk (RSM) 10% (w\/v), milk whey (MW), and soy milk whey (SMW), under optimal growth conditions for this strain. Milk whey was made by coagulating fresh milk using papaya latex 3% (v\/v); soy milk whey was obtained from tofu household industry. The chemical composition of the substrate media was determined by proximate analysis, and sterilization was accomplished in an autoclave at 121\u00b0C for 15\u00a0min. Culture media were inoculated with 1% (v\/v) of a starter culture of <jats:italic>L.\u00a0delbrueckii<\/jats:italic> subsp<jats:italic>. bulgaricus<\/jats:italic> and then incubated at 30\u00b0C for 16\u00a0hr. EPS production, lactic acid content, cell counting, and pH were determined after the media were cooled at 5\u00b0C. Findings showed that on the basis of the growth characteristics of <jats:italic>L.\u00a0delbrueckii<\/jats:italic> subsp<jats:italic>. bulgaricus<\/jats:italic>, the best medium for EPS production was RSM 10% (258.60\u00a0\u00b1\u00a026.86\u00a0mg\/L) compared to the milk whey (69.60\u00a0\u00b1\u00a09.48\u00a0mg\/L) and soy milk whey (49.80\u00a0\u00b1\u00a09.04\u00a0mg\/L).<\/jats:p>","DOI":"10.1002\/fsn3.1452","type":"journal-article","created":{"date-parts":[[2020,2,20]],"date-time":"2020-02-20T13:22:46Z","timestamp":1582204966000},"page":"1657-1664","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Assessment of exopolysaccharide production by <i>Lactobacillus delbrueckii<\/i> subsp. <i>bulgaricus<\/i> ropy strain in different substrate media"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1934-4949","authenticated-orcid":false,"given":"Ratmawati","family":"Malaka","sequence":"first","affiliation":[{"name":"Laboratory of Biotechnology of Milk Processing Department of Animal Science Faculty of Animal Science Hasanuddin University Makassar Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7976-0728","authenticated-orcid":false,"given":"Fatma","family":"Maruddin","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology of Milk Processing Department of Animal Science Faculty of Animal Science Hasanuddin University Makassar Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5002-237X","authenticated-orcid":false,"given":"Zaraswati","family":"Dwyana","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology Department of Biology Faculty of Mathematic and Natural Sciences Hasanuddin University Makassar Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maynor V.","family":"Vargas","sequence":"additional","affiliation":[{"name":"Laboratory of Chemistry and Applied Biosciences National Technical University (UTN) Alajuela Costa Rica"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,2,20]]},"reference":[{"issue":"2018","key":"e_1_2_9_2_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.btre.2018.e00271","article-title":"Characterization, antioxidant and immunomodulatory potential on exopolysaccharides produced by wild type and mutant Weissella confuse strains","volume":"19","author":"Adebayo\u2010Tayo B.","year":"2018","journal-title":"Biotechnology Reports"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2017-12761"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.905"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2015.00200"},{"issue":"3","key":"e_1_2_9_6_1","first-page":"123","article-title":"Isolation of Exopolysaccharide producing Lactobacillus bulgaricus organism from yoghurt","volume":"6","author":"Bari M. 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