{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T23:47:43Z","timestamp":1789343263021,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,5,13]],"date-time":"2021-05-13T00:00:00Z","timestamp":1620864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp; Nutrition"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Firstly, this study was designed to determine the optimal ultrasound\u2010assisted extraction parameters of <jats:italic>Saccharomyces<\/jats:italic> <jats:italic>boulardii<\/jats:italic> yeast wall polysaccharides (BYWP). Besides, the molecular weight and the ratio of mannose to glucose in compositions of BYWP were determined. Also, the effects of BYWP on the gain feed ratio, diarrhea frequency, intestinal morphology, intestinal immunity, and intestinal microbial flora of early\u2010weaned lambs were investigated. Single\u2010factor tests and Response surface optimization analysis (RSA) were used to obtain the optimal ultrasound\u2010assisted extraction conditions. Sephadex G\u2010100 column chromatography and liquid chromatography were used to analyze the molecular weight and ratio of mannose to glucose. The feeding trial was used to observe the biological functions of BYWP on early\u2010weaned lambs. A total of 72 36\u2010day\u2010old crossbred early\u2010weaned lambs were randomly divided into 4 groups with 3 replicates per group and 6 lambs per replicate. Lambs in the four treatments were fed basal milk replacer without supplement (Group I), basal milk replacer+0.1% BYWP (Group II), basal milk replacer+0.3% BYWP (Group III), and basal milk replacer+0.5% BYWP (Group IV), respectively. The optimal ultrasound\u2010assisted extraction parameters were as follows: NaOH addition: 52.63%, ultrasonic power: 143.15\u00a0W, ultrasonic time: 86.20\u00a0min, and the optimized extraction yield reached 37.54%. The molecular weights of main components BLC\u20101 and BLC\u20102 were 164.68 KDa and 13.21 KDa, and their proportions in BYWP were 24.57% and 66.08%, respectively. The proportions of glucose, mannose in BLC\u20101 and BLC\u20102 were 47.68%, 39.18%, and 76.59%, 6.86%, respectively. The addition of 0.3% and 0.5% BYWP in basal milk replacer significantly increased the average daily gain and feed conversion rate, and decreased the average fecal index and diarrhea rate of early\u2010weaned lambs. The addition of 0.3% and 0.5% BYWP significantly enhanced the intestinal morphology (villus height, crypt depth, and V\/C value) of jejunum, ileum (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). The addition of 0.3% and 0.5% BYWP significantly improved the levels of SIgA and IL\u201010, but significantly decreased the level of IL\u20101 in the ileum (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). The addition of 0.3% and 0.5% BYWP significantly increased the number of <jats:italic>Lactobacillus<\/jats:italic>, but significantly suppressed the growth of <jats:italic>Salmonella<\/jats:italic> and Clostridium <jats:italic>perfringens<\/jats:italic> (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). The results of the present study suggest that the supplementation of BYWP in the diet of early\u2010weaned lambs could increase feed utilization rate, and enhance intestinal morphology, immunological competence, microbial flora balance, and decrease the rate of diarrhea occurrence.<\/jats:p>","DOI":"10.1002\/fsn3.2318","type":"journal-article","created":{"date-parts":[[2021,5,13]],"date-time":"2021-05-13T11:52:09Z","timestamp":1620906729000},"page":"3617-3630","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Ultrasound\u2010assisted extraction of boulardii yeast cell wall polysaccharides: Characterization and its biological functions on early\u2010weaned lambs"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9776-2153","authenticated-orcid":false,"given":"Mengjian","family":"Liu","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology Shihezi University  the North 4 Road Shihezi Xinjiang 832003 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"WuJun","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science Xinjiang Agriculture University  Urumuqi Xinjiang 830000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"WenJu","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Shihezi University  the North 4 Road Shihezi Xinjiang 832003 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Yao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Shihezi University  the North 4 Road Shihezi Xinjiang 832003 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xucheng","family":"Mo","sequence":"additional","affiliation":[{"name":"College of Animal Science Xinjiang Agriculture University  Urumuqi Xinjiang 830000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,5,13]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9GC03878G"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13197-018-3148-4"},{"key":"e_1_2_10_4_1","first-page":"63","article-title":"Sijunzi Tang, Codonopsis Pilosula Polysaccharide and Saccharomyces\n                  Boulardii Modulate the Gut Microbiome to Alleviate Dss\u2010Induced Colitis in Mice","volume":"1","author":"Chen X. 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