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MO demonstrates a wide range of pharmacological effects; however, little is known about its functional constituents. To identify the anti\u2010adipogenic components of MO leaves, we prepared extracts using different extraction methods and tested the obtained extracts and fractions using ZOT. We found that the dichloromethane extract and its hexane:EtOAc\u00a0=\u00a08:2 fraction reduced VAT accumulation in young zebrafish fed a high\u2010fat diet. We also performed gene expression analysis in the zebrafish VAT and found that CCAAT\/enhancer\u2010binding protein beta and CCAAT\/enhancer\u2010binding protein delta (associated with early stages of adipogenesis) gene expression was downregulated after fraction 2 administration. We identified a new MO fraction that suppressed VAT accumulation by inhibiting early adipogenesis using the ZOT. Phenotype\u2010driven zebrafish screening is a reasonable strategy for identifying bioactive components in natural products.<\/jats:p>","DOI":"10.1002\/fsn3.2758","type":"journal-article","created":{"date-parts":[[2022,2,12]],"date-time":"2022-02-12T07:39:50Z","timestamp":1644651590000},"page":"1248-1256","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Zebrafish obesogenic test identifies anti\u2010adipogenic fraction in <i>Moringa oreifera<\/i> leaf extracts"],"prefix":"10.1002","volume":"10","author":[{"given":"Izumi","family":"Matsuoka","sequence":"first","affiliation":[{"name":"Graduate School of Regional Innovation Studies Mie University  Tsu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanae","family":"Hata","sequence":"additional","affiliation":[{"name":"Graduate School of Regional Innovation Studies Mie University  Tsu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirotaka","family":"Katsuzaki","sequence":"additional","affiliation":[{"name":"Graduate School of Bioresources Mie University  Tsu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroko","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Graduate School of Regional Innovation Studies Mie University  Tsu Japan"},{"name":"Mie University Zebrafish Drug Screening Center  Tsu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liqing","family":"Zang","sequence":"additional","affiliation":[{"name":"Graduate School of Regional Innovation Studies Mie University  Tsu Japan"},{"name":"Mie University Zebrafish Drug Screening Center  Tsu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mizuho","family":"Ota","sequence":"additional","affiliation":[{"name":"Graduate School of Bioresources Mie University  Tsu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youngil","family":"Kim","sequence":"additional","affiliation":[{"name":"Rohto Pharmaceutical Co., Ltd.  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