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We were also interested in its antioxidant, anti\u2010inflammatory, and hepatoprotective effects on carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>)\u2010induced liver injury in rats. LmPS physicochemical properties were evaluated by thin\u2010layer chromatography (TLC), high\u2010performance liquid chromatography (HPLC), thermogravimetric analysis (TGA), and UV absorption. According to TLC and HPLC results, LmPS was a heteropolysaccharide composed of glucose, galactose, and xylose. Its molecular weight was 130.62\u00a0kDa. This heteropolysaccharide was characterized by a significant antioxidant potential and was efficient against oxidative stress and CCL<jats:sub>4<\/jats:sub>\u2010induced hepatotoxicity in rat Wistar models (<jats:italic>n<\/jats:italic>\u00a0=\u00a08) treated with a single dose of LmPS 250\u00a0mg\/kg of body weight. This was evidenced by a significant increase in serum marker enzymes specially aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH). The cytokines released after stimulation of rats with LmPS showed high anti\u2010inflammatory profiles with an increased rate of interleukine\u201010 (IL\u201010) with 0.03\u00a0pg\/mL compared to animals treated only with CCl<jats:sub>4<\/jats:sub>. On the contrary, we noticed a decrease of the other cytokines (tumor necrosis factor \u03b1: TNF\u2010\u03b1, interleukine\u20106: IL\u20106, transforming growth factor\u00a0beta\u00a01: TGF\u2010\u03b21) with average concentration values of &lt;0.2, 0.1, and 0.04\u00a0pg\/mL, respectively. Besides, histopathological examinations revealed that CCl<jats:sub>4<\/jats:sub> causes acute liver damage, characterized by extensive hepatocellular necrosis, vacuolization, and inflammatory cell infiltration, as well as DNA fragmentation. LmPS administration at a dose of 250\u00a0mg\/kg resulted in a significant hepatoprotection, evidenced by a reduction of CCl<jats:sub>4<\/jats:sub>\u2010induced oxidative damage for all tested markers. These findings eagerly confirmed that LmPS was effective in the protection against CCl<jats:sub>4<\/jats:sub>\u2010induced hepatotoxicity and genotoxicity. It, therefore, suggested a potential therapeutic use of this polysaccharide as an alternative medicine for patients with acute liver diseases.<\/jats:p>","DOI":"10.1002\/fsn3.2836","type":"journal-article","created":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T07:08:12Z","timestamp":1649056092000},"page":"2271-2284","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Antioxidant and hepatoprotective effects of novel heteropolysaccharide isolated from <i>Lobularia maritima<\/i> on\u00a0CCl4\u2010induced liver injury in rats"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3837-5532","authenticated-orcid":false,"given":"Anis","family":"Ben Hsouna","sequence":"first","affiliation":[{"name":"Department of Life Sciences Faculty of Sciences of Gafsa  Gafsa Tunisia"},{"name":"Laboratory of Biotechnology and Plant Improvement Centre of Biotechnology of Sfax  Sfax 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