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The major objective of this study was to evaluate the probiotic potential of novel exopolysaccharide (EPS)\u2010producing strains of <jats:italic>S. thermophilus<\/jats:italic> isolated from Dahi, sold in the local markets of Rawalpindi and Islamabad, Pakistan. In this study, 32 isolates of <jats:italic>S. thermophilus<\/jats:italic> were initially isolated from Dahi and out of these, 10 identified strains were further screened for their EPS\u2010producing ability. Maximum EPS production was estimated for RIY strain (133.0\u00a0\u00b1\u00a00.06), followed by RIH4 strain (103.83\u00a0\u00b1\u00a00.76) and RIRT2 strain (95.77\u00a0\u00b1\u00a00.22), respectively. Thereafter, in vitro studies revealed that these newly identified EPS\u2010producing strains of <jats:italic>S. thermophilus<\/jats:italic> fulfilled the basic requirements for probiotic functions; including resistance to harsh conditions of GIT, good cell surface hydrophobicity, auto\u2010aggregation, and co\u2010aggregation, especially against <jats:italic>L. monocytogenes<\/jats:italic>. Finally, the safety assessment displayed that these strains were also sensitive to clinical antibiotics, including vancomycin. Thus, these selected EPS strains of <jats:italic>S. thermophilus<\/jats:italic> act as potential candidates for biostabilizers in the preparation of consumer\u2010friendly fermented probiotic milk products.<\/jats:p>","DOI":"10.1002\/fsn3.2843","type":"journal-article","created":{"date-parts":[[2022,3,24]],"date-time":"2022-03-24T23:36:02Z","timestamp":1648164962000},"page":"2347-2359","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["In vitro screening of EPS\u2010producing <i>Streptococcus thermophilus<\/i> strains for their probiotic potential from Dahi"],"prefix":"10.1002","volume":"10","author":[{"given":"Robina","family":"Taj","sequence":"first","affiliation":[{"name":"Institute of Food and Nutritional Sciences PMAS Arid Agricultural University Rawalpindi  Rawalpindi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tariq","family":"Masud","sequence":"additional","affiliation":[{"name":"Institute of Food and Nutritional Sciences PMAS Arid Agricultural University Rawalpindi  Rawalpindi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asma","family":"Sohail","sequence":"additional","affiliation":[{"name":"Institute of Food and Nutritional Sciences PMAS Arid Agricultural University Rawalpindi  Rawalpindi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shehla","family":"Sammi","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology The University of Haripur  Khyber Pakhtunkhwa Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rooma","family":"Naz","sequence":"additional","affiliation":[{"name":"Abbasyn University Islamabad Campus  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8550-4352","authenticated-orcid":false,"given":"Bal Kumari","family":"Sharma Khanal","sequence":"additional","affiliation":[{"name":"Ministry of Agriculture and Livestock Development Government of Nepal  Singhadurbar, Kathmandu Nepal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malik Adil","family":"Nawaz","sequence":"additional","affiliation":[{"name":"Agriculture and Food Commonwealth Scientific and Industrial Research Organisation  Werribee Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,3,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.905"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/nejmp080465"},{"key":"e_1_2_8_4_1","first-page":"257","article-title":"Antibiotic resistance and plasmid DNA contents of Streptococcus thermophilus strains isolated from Turkish yogurts","volume":"28","author":"Aslim B.","year":"2004","journal-title":"Turkish Journal of Veterinary and Animal Sciences"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362\u2010028X\u201070.1.223"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12010\u2010008\u20108183\u20106"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2009\u20102300"},{"key":"e_1_2_8_8_1","first-page":"160","article-title":"Probiotic efficiency of S. platensis stimulating growth of lactic acid bacteria","volume":"4","author":"Bhowmik D.","year":"2009","journal-title":"World Journal of Dairy Food Science"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.2298\/ABS1002323B"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022\u20100302(98)75785\u20106"},{"key":"e_1_2_8_11_1","first-page":"1268","volume-title":"Bergey\u2019s manual of determinative bacteriology","author":"Buchanan R. 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