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Given the widespread interest about antimicrobial susceptibilities, transmission of resistances, and virulence factors, there is little research available on such topics for <jats:italic>Weissella<\/jats:italic>. The aim of this study was to assess the safety aspects and antimicrobial potential of 54 <jats:italic>Weissella<\/jats:italic> spp. strains from different environmental sources. Antibiotic susceptibility, hemolytic activity, horizontal transfer, and antibacterial activity were studied, as well as the detection of biogenic amine BA production on decarboxylase medium and PCR was performed. All the strains were nonhemolytic and sensitive to chloramphenicol and ampicillin. Several strains were classified as resistant to fusidic acid, and very low resistance rates were detected to ciprofloxacin, tetracycline, streptomycin, lincomycin, erythromycin, and rifampicin, although all strains had intrinsic resistance to vancomycin, nalidixic acid, kanamycin, and teicoplanin. Two BA\u2010producing strains (<jats:italic>W<\/jats:italic>.\u00a0<jats:italic>halotolerans<\/jats:italic> FAS30 and FAS29) exhibited tyrosine decarboxylase activity, and just one <jats:italic>W<\/jats:italic>.\u00a0<jats:italic>confusa<\/jats:italic> FS077 produced both tyramine and histamine, and their genetic determinants were identified. Ornithine decarboxylase\/<jats:italic>odc<\/jats:italic> gene was found in 16 of the <jats:italic>Weissella<\/jats:italic> strains, although 3 of them synthesize putrescine. Interestingly, eight strains with good properties displayed antibacterial activity. Conjugation frequencies of erythromycin from <jats:italic>Bacillus<\/jats:italic> to <jats:italic>Weissella<\/jats:italic> spp. varied in the average of 3\u00a0\u00d7\u00a010<jats:sup>\u22129<\/jats:sup> transconjugants\/recipient. However, no tetracycline\u2010resistant transconjugant was obtained with <jats:italic>Enterococcus faecalis<\/jats:italic> JH2\u20102 as recipient. The obtained results support the safe status of <jats:italic>Weissella<\/jats:italic> strains, derived from environmental sources, when used as probiotics in animal feed.<\/jats:p>","DOI":"10.1002\/fsn3.2885","type":"journal-article","created":{"date-parts":[[2022,4,16]],"date-time":"2022-04-16T06:53:08Z","timestamp":1650091988000},"page":"2896-2910","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Antimicrobial activity and safety features assessment of <i>Weissella<\/i> spp. from environmental sources"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6416-685X","authenticated-orcid":false,"given":"Imene","family":"Fhoula","sequence":"first","affiliation":[{"name":"Laboratoire Microorganismes et Biomol\u00e9cules Actives (LR03ES03) Facult\u00e9 des Sciences de Tunis Universit\u00e9 Tunis El Manar  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7686-6722","authenticated-orcid":false,"given":"Mohamed","family":"Boumaiza","sequence":"additional","affiliation":[{"name":"Laboratoire Microorganismes et Biomol\u00e9cules Actives (LR03ES03) Facult\u00e9 des Sciences de Tunis Universit\u00e9 Tunis El Manar  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghassan","family":"Tayh","sequence":"additional","affiliation":[{"name":"Laboratoire Microorganismes et Biomol\u00e9cules Actives (LR03ES03) Facult\u00e9 des Sciences de Tunis Universit\u00e9 Tunis El Manar  Tunis Tunisia"},{"name":"Service de Microbiologie et d\u2019Immunologie Ecole Nationale de M\u00e9decine V\u00e9t\u00e9rinaire Universit\u00e9 Manouba  Sidi Thabet Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amel","family":"Rehaiem","sequence":"additional","affiliation":[{"name":"Faculty of Medicine of Tunis Research Laboratory \u201cAntimicrobial Resistance\u201d LR99ES09 University of Tunis El Manar  Tunis Tunisia"},{"name":"Laboratory of Microbiology Charles Nicolle Hospital  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naouel","family":"Klibi","sequence":"additional","affiliation":[{"name":"Laboratoire Microorganismes et Biomol\u00e9cules Actives (LR03ES03) Facult\u00e9 des Sciences de Tunis Universit\u00e9 Tunis El Manar  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3237-554X","authenticated-orcid":false,"given":"Imene\u2010Hadda","family":"Ouzari","sequence":"additional","affiliation":[{"name":"Laboratoire Microorganismes et Biomol\u00e9cules Actives (LR03ES03) Facult\u00e9 des Sciences de Tunis Universit\u00e9 Tunis El Manar  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0732\u20108893(00)00130\u20109"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.01197"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfpp.14468"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.5772\/intechopen.80624"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2006.11.001"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2011.03.014"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods8010017"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13213\u2010015\u20101072\u20107"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.15414\/jmbfs.2015.4.6.513\u2010517"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/1541\u20104337.12212"},{"key":"e_1_2_9_12_1","first-page":"643","article-title":"Genus III. 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