{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T01:43:51Z","timestamp":1786239831625,"version":"build-2736575974"},"reference-count":54,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,8,9]],"date-time":"2022-08-09T00:00:00Z","timestamp":1660003200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["41806173"],"award-info":[{"award-number":["41806173"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp; Nutrition"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Chukrasia tabularis<\/jats:italic> is an economically important tree and widely cultured in the southeast of China. Its barks, leaves, and fruits are consumed as a traditional medicine and perceived as a valuable source for bioactive limonin compounds. The extracts from root barks of <jats:italic>C.\u00a0tabularis<\/jats:italic> showed significant anti\u2010inflammatory effect. The aim of this research was to explore the material basis of <jats:italic>C.\u00a0tabularis<\/jats:italic> anti\u2010inflammatory activity, and to purify and identify anti\u2010inflammatory active ingredients. By a bioassay\u2010guided isolation of dichloromethane fraction obtained two novel phragmalin limonins, Chukrasitin D and E (<jats:bold>1<\/jats:bold> and <jats:bold>2<\/jats:bold>), together with 12 known limonins (<jats:bold>3<\/jats:bold>\u2013<jats:bold>14<\/jats:bold>). The chemical structure of these compounds is determined on the basis of extensive spectral analysis and chemical reactivity. In addition, the activities of these isolated limonins on the production of nitric oxide (NO), tumor necrosis factor alpha (TNF\u2010\u03b1), and nuclear factor kappa B (NF\u2010<jats:italic>\u03ba<\/jats:italic>B) in RAW264.7 cells induced by lipopolysaccharide (LPS) were evaluated. Limonins <jats:bold>1<\/jats:bold> and <jats:bold>2<\/jats:bold> indicated significant anti\u2010inflammatory activity with IC<jats:sub>50<\/jats:sub> values of 6.24 and 6.13\u00a0<jats:italic>\u03bc<\/jats:italic>M. Compound <jats:bold>1<\/jats:bold> notably inhibited the production of NF\u2010<jats:italic>\u03ba<\/jats:italic>B, TNF\u2010<jats:italic>\u03b1<\/jats:italic> and interleukin 6 (IL\u20106) in macrophages<jats:italic>.<\/jats:italic> The present results suggest that the root barks of <jats:italic>C.\u00a0tabularis<\/jats:italic> exhibited anti\u2010inflammatory effect and the limonins may be responsible for this activity.<\/jats:p>","DOI":"10.1002\/fsn3.3015","type":"journal-article","created":{"date-parts":[[2022,8,10]],"date-time":"2022-08-10T04:59:25Z","timestamp":1660107565000},"page":"4216-4225","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Bioactivity\u2010guided isolation of anti\u2010inflammatory limonins from <i>Chukrasia tabularis<\/i>"],"prefix":"10.1002","volume":"10","author":[{"given":"Jin\u2010Huang","family":"Shen","sequence":"first","affiliation":[{"name":"Fujian Key Laboratory of Natural 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