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We were able to examine and accurately demonstrate its permeability effect in vitro and its differentiated effect on tight junction expression in different segments of the intestine in vivo using cultured intestinal porcine epithelial cell line (IPEC\u2010J2) and piglets. A cultured IPEC\u2010J2 demonstrated that \u03b1\u2010tocopherol upregulated the expression of tight junction proteins and improved their integrity, with a maximum effect at concentrations ranging from 20 to 40\u2009\u03bcmol\/L. In vivo data from weaned pigs fed different doses of \u03b1\u2010tocopherol for 2\u2009weeks revealed that \u03b1\u2010tocopherol effectively increases the expression of tight junction proteins in all sections of the intestinal mucosa, with the highest effect on the duodenum at an optimum dose of 20\u201350\u2009mg\/kg. In contrast, \u03b1\u2010tocopherol did not affect intestinal inflammation. These findings suggest that \u03b1\u2010tocopherol maintains intestinal integrity and increases the expression of tight junction proteins both in vitro and in vivo.<\/jats:p>","DOI":"10.1002\/fsn3.3103","type":"journal-article","created":{"date-parts":[[2022,10,30]],"date-time":"2022-10-30T14:20:00Z","timestamp":1667139600000},"page":"677-687","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The beneficial effects of alpha\u2010tocopherol on intestinal function and the expression of tight junction proteins in differentiated segments of the intestine in piglets"],"prefix":"10.1002","volume":"11","author":[{"given":"Yanjun","family":"Huang","sequence":"first","affiliation":[{"name":"Key Laboratory for Study and Discovery of Small Targeted Molecules of Hunan Province Department of Pharmacy, School of Medicine Hunan Normal University Changsha 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