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The objective of this work was to investigate the antimicrobial resistance (AMR) patterns, virulence genes, and genetic variation of thermophilic<jats:italic>Campylobacter<\/jats:italic>species collected from chicken meat samples in Iran. A total of 255 meat specimens were taken and transferred to the laboratory. Culture methods were utilized to identify the<jats:italic>Campylobacter<\/jats:italic>genus, and PCR and sequencing were performed to confirm the organisms. Antimicrobial susceptibility evaluation was performed using broth microdilution for six antimicrobials [ciprofloxacin (CIP), nalidixic acid (NAL), sitafloxacin (SIT), erythromycin (ERY), tetracycline (TET), and gentamicin (GEN)]. By using PCR, AMR and virulence genes were detected. The detection rate of<jats:italic>Campylobacter<\/jats:italic>spp. was 64 (25.09%) out of 255 meat samples, with<jats:italic>C.\u00a0jejuni<\/jats:italic>and<jats:italic>C.\u00a0coli<\/jats:italic>accounting for 41 (64.06%) and 14 (21.87%), respectively. Other<jats:italic>Campylobacter<\/jats:italic>isolates accounted for 14.06% of the total (nine samples). The antibiotic susceptibility of all<jats:italic>Campylobacter<\/jats:italic>isolates was tested using six antibiotics, and all (100%) were resistant to CIP and NAL. However, TET resistance was observed in 93.9% and 83.3% of<jats:italic>C.\u00a0jejuni<\/jats:italic>and<jats:italic>C.\u00a0coli<\/jats:italic>isolates, respectively. Four (8.2%)<jats:italic>C.\u00a0jejuni<\/jats:italic>isolates were multidrug\u2010resistant (MDR), while none of the<jats:italic>C.\u00a0coli<\/jats:italic>isolates were MDR. Two of the four MDR isolates were resistant to CIP, NAL, TET, and ERY, whereas the other two isolates were resistant to CIP, NAL, TET, and GEN. The values of the Minimum Inhibitory Concentration (MIC) were as follows: CIP, 64\u2013256\u2009\u03bcg\/ml; NAL, 128\u2013512\u2009\u03bcg\/ml; TET, 2\u20131024\u2009\u03bcg\/ml; SIT, 0.25\u20131\u00a0\u03bcg\/ml; ERY, 1\u201332\u2009\u03bcg\/ml; and GEN, 1\u2013256\u2009\u03bcg\/ml.<jats:italic>recR<\/jats:italic>,<jats:italic>dnaJ<\/jats:italic>,<jats:italic>cdtC<\/jats:italic>,<jats:italic>cdtB<\/jats:italic>,<jats:italic>cdtA<\/jats:italic>,<jats:italic>flaA<\/jats:italic>,<jats:italic>ciaB<\/jats:italic>,<jats:italic>cadF<\/jats:italic>, and<jats:italic>pidA<\/jats:italic>were discovered in more than 50% of<jats:italic>C.\u00a0jejuni<\/jats:italic>isolates, although<jats:italic>wlaN<\/jats:italic>,<jats:italic>virbll<\/jats:italic>,<jats:italic>cgtB<\/jats:italic>, and<jats:italic>ceuE<\/jats:italic>were found in &lt;50%.<jats:italic>flaA<\/jats:italic>,<jats:italic>cadF<\/jats:italic>,<jats:italic>pidA<\/jats:italic>, and<jats:italic>ciaB<\/jats:italic>were discovered in more than 50% of the<jats:italic>C.\u00a0coli<\/jats:italic>samples, whereas<jats:italic>recR<\/jats:italic>,<jats:italic>cdtC<\/jats:italic>,<jats:italic>cdtB<\/jats:italic>,<jats:italic>cdtA<\/jats:italic>, and<jats:italic>cgtB<\/jats:italic>were found in less than half. For<jats:italic>C.\u00a0coli<\/jats:italic>, the percentages for<jats:italic>wlaN<\/jats:italic>,<jats:italic>dnaJ<\/jats:italic>,<jats:italic>virbll<\/jats:italic>, and<jats:italic>ceuE<\/jats:italic>were all zero. The results of this study show<jats:italic>Campylobacter<\/jats:italic>isolates obtained from poultry have higher resistance to quinolones and TET, pathogenicity potential, and varied genotypes.<\/jats:p>","DOI":"10.1002\/fsn3.3152","type":"journal-article","created":{"date-parts":[[2022,11,21]],"date-time":"2022-11-21T10:14:53Z","timestamp":1669025693000},"page":"1142-1153","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["<i>Campylobacter<\/i>spp. isolated from poultry in Iran: Antibiotic resistance profiles, virulence genes, and molecular mechanisms"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3101-6035","authenticated-orcid":false,"given":"Seyedeh Bita","family":"Mousavinafchi","sequence":"first","affiliation":[{"name":"Department of Food Hygiene, Faculty of Veterinary Medicine, Shahrekord Branch Islamic Azad University Shahrekord Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6451-2297","authenticated-orcid":false,"given":"Ebrahim","family":"Rahimi","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene, Faculty of Veterinary Medicine, Shahrekord Branch Islamic Azad University Shahrekord Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4154-5456","authenticated-orcid":false,"given":"Amir","family":"Shakerian","sequence":"additional","affiliation":[{"name":"Research Center of Nutrition and Organic Products, Shahrekord Branch Islamic Azad University Shahrekord Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,11,21]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02765-19"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.2020.1857887"},{"issue":"1","key":"e_1_2_11_4_1","first-page":"15","article-title":"Virulence and antimicrobial resistance pattern in Campylobacter jejuni and Campylobacter coli strains isolated from the liver of slaughtered broiler chickens in Chaharmahal va Bakhtiari province","volume":"2","author":"Banisharif G.","year":"2019","journal-title":"New Findings in Veterinary Microbiology"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/vms3.754"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2020.108984"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268813002410"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.1937"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2001.01358.x"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2020.04.009"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.05056-0"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.33448\/rsd-v10i10.19224"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01014"},{"issue":"2","key":"e_1_2_11_14_1","first-page":"E79","article-title":"Campylobacter: From microbiology to prevention","volume":"58","author":"Facciol\u00e0 A.","year":"2017","journal-title":"Journal of Preventive Medicine and Hygiene"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02491"},{"key":"e_1_2_11_16_1","doi-asserted-by":"crossref","first-page":"e202100426","DOI":"10.1002\/cbdv.202100426","article-title":"Fabrication and characterization of thymol\u2010loaded chitosan nanogels: Improved antibacterial and anti\u2010biofilm activities with negligible cytotoxicity","volume":"19","author":"Gharaghie T. 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