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Vc contamination is widely found in water and aquatic products, and therefore is a serious food safety concern, especially for the seafood industry. In this paper, we attempted the rapid detection of <jats:italic>V. cholerae<\/jats:italic>. Nine rounds of in vitro selection using an unmodified DNA library were successfully performed to find specific DNAzymes of Vc. Their activity was evaluated based on a fluorescence assay and gel electrophoresis. Finally, a DNAzyme (named DVc1) with good activity and specificity with a detection limit of 7.2\u2009\u00d7\u200910<jats:sup>3<\/jats:sup>\u2009CFU\/mL of Vc was selected. A simple biosensor was constructed by immobilizing DVc1 and its substrate in shallow circular wells of a 96\u2010well plate using pullulan polysaccharide and trehalose. When the crude extracellular mixture of Vc was added to the detection wells, the fluorescent signal was observed within 20\u2009min. The sensor effectively detected Vc in aquatic products indicating its simplicity and efficiency. This sensitive DNAzyme sensor can be a rapid onsite Vc detection tool.<\/jats:p>","DOI":"10.1002\/fsn3.3304","type":"journal-article","created":{"date-parts":[[2023,3,13]],"date-time":"2023-03-13T11:41:30Z","timestamp":1678707690000},"page":"3235-3245","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["An efficient <scp>DNAzyme<\/scp> for the fluorescence detection of <i>Vibrio cholerae<\/i>"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3055-2335","authenticated-orcid":false,"given":"Qingzhen","family":"Miao","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Marine Bioresources and Environment\/Jiangsu Key Laboratory of Marine Biotechnology Jiangsu Ocean University  Lianyungang China"},{"name":"Co\u2010Innovation Center of Jiangsu Marine Bio\u2010industry Technology Jiangsu Ocean University  Lianyungang 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