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To achieve this, we conducted an in\u00a0vitro analysis of several key probiotic properties exhibited by the 9\u2009N\u20102 strain. Notably, this strain demonstrated robust resilience to low pH, high bile salt concentrations, lysozyme, pepsin, pancreatin, and phenol. Furthermore, this strain displayed exceptional auto\u2010aggregation capabilities and moderate co\u2010aggregation tendencies when interacting with <jats:italic>Escherichia coli<\/jats:italic>. The cell\u2010free supernatant derived from strain 9\u2009N\u20102 exhibited significant antimicrobial activity against the tested pathogens. The strain exhibited resistance to gentamicin, meropenem, and bacitracin, while remaining susceptible to vancomycin and various other antibiotics. Furthermore, it was found that <jats:italic>E.\u2009faecium<\/jats:italic> 9\u2009N\u20102 possessed the capacity to produce the phytase enzyme. When all the results of this study are evaluated, it is thought that 9\u2009N\u20102 strain has superior probiotic properties, and therefore it can be used as probiotic in food, medicine, and animal feed in the future. In addition, further in\u00a0vivo tests should be performed to fully understand its effects and mechanisms of action and to confirm its safety and probiotic effects. Further research and clinical trials are also needed to identify new strains with potential probiotic properties.<\/jats:p>","DOI":"10.1002\/fsn3.3878","type":"journal-article","created":{"date-parts":[[2023,11,29]],"date-time":"2023-11-29T06:53:20Z","timestamp":1701240800000},"page":"1847-1856","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Evaluating <i>Enterococcus faecium<\/i><scp>9\u2009N<\/scp>\u20102 as a probiotic candidate from traditional village white cheese"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9096-2761","authenticated-orcid":false,"given":"Neslihan","family":"Dikba\u015f","sequence":"first","affiliation":[{"name":"Department of Agricultural Biotechnology, Agricultural Faculty Ataturk University  Erzurum Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuf 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